Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Mao, G.; Zhao, Y.; Kang, X.; Li, Z.; Zhang, Y.; Wang, X.; Sun, F.; Sankaran, K.; Zhang, X.C.
    Crystal structure of E. coli lipoprotein diacylglyceryl transferase (2016), Nat. Commun., 7, 10198 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.145 expressed in Escherichia coli CE43 (DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.145 in complex with phosphatidylglycerol and palmitic acid, hanging drop vapor diffusion method, using 0.1 M Tris (pH 6.5), 0.1 M CaCl2 and 13% (w/v) PEG2000 MME Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.145 D88N the mutation has no effect on enzyme activity Escherichia coli
2.5.1.145 D97N the mutation has no effect on enzyme activity Escherichia coli
2.5.1.145 E151A inactive Escherichia coli
2.5.1.145 E151Q inactive Escherichia coli
2.5.1.145 G138I inactive Escherichia coli
2.5.1.145 G138V inactive Escherichia coli
2.5.1.145 G142A inactive Escherichia coli
2.5.1.145 G142I inactive Escherichia coli
2.5.1.145 G142V inactive Escherichia coli
2.5.1.145 G145A inactive Escherichia coli
2.5.1.145 G145I inactive Escherichia coli
2.5.1.145 G145V inactive Escherichia coli
2.5.1.145 G27L inactive Escherichia coli
2.5.1.145 G27Q inactive Escherichia coli
2.5.1.145 G27W inactive Escherichia coli
2.5.1.145 R143A inactive Escherichia coli
2.5.1.145 Y26A inactive Escherichia coli
2.5.1.145 Y26F inactive Escherichia coli
2.5.1.145 Y26Q inactive Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.145 n-octyl-beta-D-glucoside
-
 Escherichia coli
2.5.1.145 palmitic acid
-
 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.145 membrane
-
 Escherichia coli 16020
-

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.145 Escherichia coli P60955
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.145 HiTrap nickel column chromatography and Superdex 200 gel filtration Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.145 dipalmitoyl phosphatidic acid + MKATKLVLGAVILGSTLLAGCSSN weak activity Escherichia coli ?
-
 ?
2.5.1.145 dipalmitoyl phosphatidyl-L-serine + MKATKLVLGAVILGSTLLAGCSSN weak activity Escherichia coli ?
-
 ?
2.5.1.145 dipalmitoyl-L-1-phosphatidyl-sn-glycerol + MKATKLVLGAVILGSTLLAGCSSN
-
 Escherichia coli dipalmitoyl-sn-glycerol 1-phosphate + MKATKLVLGAVILGSTLLAGC-S-1,2-diacyl-sn-glyceryl-L-cysteine-SSN
-
 ?
2.5.1.145 L-1-phosphatidyl-sn-glycerol + MKATKLVLGAVILGSTLLAGCSSN
-
 Escherichia coli sn-glycerol 1-phosphate + MKATKLVLGAVILGSTLLAGC-S-1,2-diacyl-sn-glyceryl-L-cysteine-SSN
-
 ?
2.5.1.145 additional information neutral phospholipid, dipalmitoyl phosphatidyl-ethanolamine, dipalmitoyl phosphatidyl-choline and diacylglycerol are no substrates Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.145 LGT
-
 Escherichia coli
2.5.1.145 lipoprotein diacylglyceryl transferase
-
 Escherichia coli
2.5.1.145 phosphatidylglycerol:prolipoproteindiacylglyceryl transferase
-
 Escherichia coli