EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.99.16 | recombinant enzyme expression in Escherichia coli strain BL21(DE3) | Thermus thermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.4.99.16 | additional information | the crude TtTreS is immobilized on the surface of glutaraldehyde-3-aminopropyltriethoxysilane-silicalite-1 (GA-APS-silicalite-1, silicalite-1 modified sequentially with 3-aminopropyltriethoxysilane and glutaraldehyde) without enzyme purification. In this case, a nucleophilic attack takes place at the aldehyde group of glutaraldehyde by the amino group of the protein to form a Schiff base. Preparation of activated silicalite-1, APS-silicalite-1, and GA-APS-silicalite-1 and enzyme immobilization, method optimization, overview. Immobilization also provides TtTreS with potential application in a relatively wide range of temperatures from 40°C to 70°C. The trehalose yield of immobilized TtTreS in first cycle reaches 61.52%. After 22 cycles of enzymatic reaction, the immobilized TtTreS still retains 81% of its initial trehalose yield | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.99.16 | 14.12 | - |
maltose | recombinant free enzyme, pH 8.0, 50°C | Thermus thermophilus | |
5.4.99.16 | 53.33 | - |
maltose | recombinant immobilized enzyme, pH 8.0, 50°C | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.16 | maltose | Thermus thermophilus | - |
alpha,alpha-trehalose | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.16 | Thermus thermophilus | O06458 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.16 | maltose | - |
Thermus thermophilus | alpha,alpha-trehalose | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.16 | Trehalose synthase | - |
Thermus thermophilus |
5.4.99.16 | TtTreS | - |
Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.99.16 | 50 | - |
free and immobilized recombinant enzyme | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.16 | 8 | - |
immobilized recombinant enzyme | Thermus thermophilus |
5.4.99.16 | 9 | - |
free recombinant enzyme | Thermus thermophilus |