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Literature summary extracted from

  • Sun, J.; Wang, S.; Li, W.; Li, R.; Chen, S.; Ri, H.; Kim, T.; Kang, M.; Sun, L.; Sun, X.; Yuan, Q.
    Improvement of trehalose production by immobilized trehalose synthase from Thermus thermophilus HB27 (2018), Molecules, 23, pii: E1087 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.16 recombinant enzyme expression in Escherichia coli strain BL21(DE3) Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.16 additional information the crude TtTreS is immobilized on the surface of glutaraldehyde-3-aminopropyltriethoxysilane-silicalite-1 (GA-APS-silicalite-1, silicalite-1 modified sequentially with 3-aminopropyltriethoxysilane and glutaraldehyde) without enzyme purification. In this case, a nucleophilic attack takes place at the aldehyde group of glutaraldehyde by the amino group of the protein to form a Schiff base. Preparation of activated silicalite-1, APS-silicalite-1, and GA-APS-silicalite-1 and enzyme immobilization, method optimization, overview. Immobilization also provides TtTreS with potential application in a relatively wide range of temperatures from 40°C to 70°C. The trehalose yield of immobilized TtTreS in first cycle reaches 61.52%. After 22 cycles of enzymatic reaction, the immobilized TtTreS still retains 81% of its initial trehalose yield Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.16 14.12
-
maltose recombinant free enzyme, pH 8.0, 50°C Thermus thermophilus
5.4.99.16 53.33
-
maltose recombinant immobilized enzyme, pH 8.0, 50°C Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.16 maltose Thermus thermophilus
-
alpha,alpha-trehalose
-
r

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.16 Thermus thermophilus O06458
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.16 maltose
-
Thermus thermophilus alpha,alpha-trehalose
-
r

Synonyms

EC Number Synonyms Comment Organism
5.4.99.16 Trehalose synthase
-
Thermus thermophilus
5.4.99.16 TtTreS
-
Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.16 50
-
free and immobilized recombinant enzyme Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.16 8
-
immobilized recombinant enzyme Thermus thermophilus
5.4.99.16 9
-
free recombinant enzyme Thermus thermophilus