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Literature summary extracted from

  • Ashenafi, M.; Reddy, P.T.; Parsons, J.F.; Byrnes, W.M.
    The fused anthranilate synthase from Streptomyces venezuelae functions as a monomer (2015), Mol. Cell. Biochem., 400, 9-15 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.27 expressed in Escherichia coli BL21(DE3) cells Streptomyces venezuelae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.27 tryptophan the enzyme is competitively but non-cooperatively inhibited by tryptophan Streptomyces venezuelae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.27 0.00084
-
 L-glutamine at pH 7.5 and 30°C Streptomyces venezuelae
4.1.3.27 0.0082
-
 chorismate at pH 7.5 and 30°C Streptomyces venezuelae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.27 66000
-
 gel filtration Streptomyces venezuelae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.27 chorismate + L-glutamine Streptomyces venezuelae
-
 anthranilate + pyruvate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 Streptomyces venezuelae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.27 Ni-NTA column chromatography Streptomyces venezuelae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + L-glutamine
-
 Streptomyces venezuelae anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 additional information the enzyme does not utilize ammonia as a substrate Streptomyces venezuelae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.27 monomer 1 * 67000, SDS-PAGE Streptomyces venezuelae
4.1.3.27 monomer 1 * 66600, laser light scattering Streptomyces venezuelae

Synonyms

EC Number Synonyms Comment Organism
4.1.3.27 TrpE
-
 Streptomyces venezuelae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.27 45
-
-
 Streptomyces venezuelae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.27 0.57
-
 L-glutamine at pH 7.5 and 30°C Streptomyces venezuelae
4.1.3.27 0.57
-
 chorismate at pH 7.5 and 30°C Streptomyces venezuelae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.27 7.5
-
-
 Streptomyces venezuelae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.3.27 0.0111
-
 tryptophan at pH 7.5 and 30°C Streptomyces venezuelae