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Literature summary extracted from

  • Lu, Y.; Zhang, Y.; Grimes, K.; Qi, J.; Lee, R.; Rock, C.
    Acyl-phosphates initiate membrane phospholipid synthesis in Gram-positive pathogens (2006), Mol. Cell., 23, 765-772 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.275 pharmacology the PlsX/Y pathway defines the most widely distributed pathway for the initiation of phospholipid formation in bacteria and represents a target for the development of antibacterial therapeutics Streptococcus pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.274 expression in Escherichia coli Streptococcus pneumoniae
2.3.1.275 expression in Escherichia coli Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.274 0.14
-
 phosphate pH 7.4, 37°C Streptococcus pneumoniae
2.3.1.274 0.3
-
 hexadecanoyl-[acyl-carrier protein] pH 7.4, 37°C Streptococcus pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.275 membrane transmembrane protein with five membrane-spanning helices Streptococcus pneumoniae 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.274 Mg2+ activity is modestly enhanced by Mg2+ but does not require a divalent cation Streptococcus pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.274 Streptococcus pneumoniae Q8DRN3
-
-
2.3.1.274 Streptococcus pneumoniae ATCC BAA-255 Q8DRN3
-
-
2.3.1.275 Streptococcus pneumoniae P0A4Q0
-
-
2.3.1.275 Streptococcus pneumoniae ATCC BAA-255 P0A4Q0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.274
-
 Streptococcus pneumoniae
2.3.1.275 recombinant His-tagged SpPlsY Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.274 hexadecanoyl-[acyl-carrier protein] + phosphate the enzyme is involved in phosphatidic acid synthesis Streptococcus pneumoniae hexadecanoyl phosphate + an [acyl-carrier protein]
-
 ?
2.3.1.274 hexadecanoyl-[acyl-carrier protein] + phosphate the enzyme is involved in phosphatidic acid synthesis Streptococcus pneumoniae ATCC BAA-255 hexadecanoyl phosphate + an [acyl-carrier protein]
-
 ?
2.3.1.274 additional information the enzyme is specific for acyl-ACP, and acyl-CoA and free fatty acid are not substrates Streptococcus pneumoniae ?
-
 ?
2.3.1.274 additional information the enzyme is specific for acyl-ACP, and acyl-CoA and free fatty acid are not substrates Streptococcus pneumoniae ATCC BAA-255 ?
-
 ?
2.3.1.275 palmitoyl phosphate + sn-glycerol 3-phosphate
-
 Streptococcus pneumoniae 1-palmitoyl-sn-glycerol 3-phosphate + phosphate
-
 ?
2.3.1.275 palmitoyl phosphate + sn-glycerol 3-phosphate
-
 Streptococcus pneumoniae ATCC BAA-255 1-palmitoyl-sn-glycerol 3-phosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.274 plsX
-
 Streptococcus pneumoniae
2.3.1.274 SPR0037
-
 Streptococcus pneumoniae
2.3.1.275 SpPlsY protein
-
 Streptococcus pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.275 37
-
 assay at Streptococcus pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.275 7.4
-
 assay at Streptococcus pneumoniae