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Literature summary extracted from
- Evolution of eukaryal and archaeal pseudouridine synthase Pus10 (2018), J. Mol. Evol., 86, 77-89 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.25 | gene PUS10, genotyping, phylogenetic analysis | Homo sapiens |
| 5.4.99.25 | gene PUS10, genotyping, phylogenetic analysis | Methanocaldococcus jannaschii |
| 5.4.99.B25 | gene PUS10, genotyping, phylogenetic analysis | Homo sapiens |
| 5.4.99.B25 | gene PUS10, genotyping, phylogenetic analysis | Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.B25 | tRNA uridine54 | Homo sapiens | - |
tRNA pseudouridine54 | - |
? |  |
| 5.4.99.B25 | tRNA uridine54 | Methanocaldococcus jannaschii | - |
tRNA pseudouridine54 | - |
? | |
| 5.4.99.25 | tRNA uridine55 | Homo sapiens | - |
tRNA pseudouridine55 | - |
? | |
| 5.4.99.25 | tRNA uridine55 | Methanocaldococcus jannaschii | - |
tRNA pseudouridine55 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.B22 | Arabidopsis thaliana | F4HSS8 | - |
- |
| 5.4.99.B22 | Homo sapiens | Q3MIT2 | - |
- |
| 5.4.99.B22 | Methanocaldococcus jannaschii | Q60346 | - |
- |
| 5.4.99.B22 | Pyrococcus furiosus | Q8U1R6 | - |
- |
| 5.4.99.25 | Homo sapiens | Q3MIT2 | - |
- |
| 5.4.99.B25 | Homo sapiens | Q3MIT2 | - |
- |
| 5.4.99.25 | Methanocaldococcus jannaschii | Q60346 | - |
- |
| 5.4.99.B25 | Methanocaldococcus jannaschii | Q60346 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.B25 | tRNA uridine54 | - |
Homo sapiens | tRNA pseudouridine54 | - |
? | |
| 5.4.99.B25 | tRNA uridine54 | - |
Methanocaldococcus jannaschii | tRNA pseudouridine54 | - |
? | |
| 5.4.99.25 | tRNA uridine55 | - |
Homo sapiens | tRNA pseudouridine55 | - |
? | |
| 5.4.99.25 | tRNA uridine55 | - |
Methanocaldococcus jannaschii | tRNA pseudouridine55 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.25 | More | homology modeling and structural superimposition, overview | Methanocaldococcus jannaschii |
| 5.4.99.B25 | More | homology modeling and structural superimposition, overview | Methanocaldococcus jannaschii |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.B22 | pseudouridine synthase Pus10 | - |
Homo sapiens |
| 5.4.99.B22 | pseudouridine synthase Pus10 | - |
Methanocaldococcus jannaschii |
| 5.4.99.B22 | pseudouridine synthase Pus10 | - |
Arabidopsis thaliana |
| 5.4.99.B22 | pseudouridine synthase Pus10 | - |
Pyrococcus furiosus |
| 5.4.99.25 | More | cf. tRNA pseudouridine54 synthase, EC 5.4.99 | Homo sapiens |
| 5.4.99.25 | More | cf. tRNA pseudouridine54 synthase, EC 5.4.99 | Methanocaldococcus jannaschii |
| 5.4.99.B25 | More | cf. EC 5.4.99.25 | Homo sapiens |
| 5.4.99.B25 | More | cf. EC 5.4.99.25 | Methanocaldococcus jannaschii |
| 5.4.99.25 | pseudouridine synthase | - |
Homo sapiens |
| 5.4.99.25 | pseudouridine synthase | - |
Methanocaldococcus jannaschii |
| 5.4.99.B25 | pseudouridine synthase | - |
Homo sapiens |
| 5.4.99.B25 | pseudouridine synthase | - |
Methanocaldococcus jannaschii |
| 5.4.99.25 | pseudouridine synthase Pus10 | - |
Homo sapiens |
| 5.4.99.25 | pseudouridine synthase Pus10 | - |
Methanocaldococcus jannaschii |
| 5.4.99.B25 | pseudouridine synthase Pus10 | - |
Homo sapiens |
| 5.4.99.B25 | pseudouridine synthase Pus10 | - |
Methanocaldococcus jannaschii |
| 5.4.99.25 | PSI synthase | - |
Homo sapiens |
| 5.4.99.25 | PSI synthase | - |
Methanocaldococcus jannaschii |
| 5.4.99.B25 | PSI synthase | - |
Homo sapiens |
| 5.4.99.B25 | PSI synthase | - |
Methanocaldococcus jannaschii |
| 5.4.99.25 | Pus10 | - |
Homo sapiens |
| 5.4.99.25 | Pus10 | - |
Methanocaldococcus jannaschii |
| 5.4.99.B25 | Pus10 | - |
Homo sapiens |
| 5.4.99.B25 | Pus10 | - |
Methanocaldococcus jannaschii |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.4.99.B22 | evolution | eukaryal Pus10 genes share a conserved catalytic domain with archaeal Pus10 genes. Pus10 is found in earlier evolutionary branches of fungi (such as chytrid Batrachochytrium) but is absent in all dikaryon fungi surveyed (Ascomycetes and Basidiomycetes). Orthologs of Pus10, TrmA, and TruB are present in all the animals, plants, and protozoa surveyed. This indicates that the common eukaryotic ancestor possesses all the three genes. Pus10 exists as a single copy gene in all the surveyed genomes despite ancestral whole genome duplications has occurred. This indicates a possible deleterious gene dosage effect. Functional redundancy results in gene loss or neofunctionalization in different evolutionary lineages | Homo sapiens |
| 5.4.99.B22 | evolution | eukaryal Pus10 genes share a conserved catalytic domain with archaeal Pus10 genes. Pus10 is found in earlier evolutionary branches of fungi (such as chytrid Batrachochytrium) but is absent in all dikaryon fungi surveyed (Ascomycetes and Basidiomycetes). Orthologs of Pus10, TrmA, and TruB are present in all the animals, plants, and protozoa surveyed. This indicates that the common eukaryotic ancestor possesses all the three genes. Pus10 exists as a single copy gene in all the surveyed genomes despite ancestral whole genome duplications has occurred. This indicates a possible deleterious gene dosage effect. Functional redundancy results in gene loss or neofunctionalization in different evolutionary lineages | Methanocaldococcus jannaschii |
| 5.4.99.B22 | evolution | eukaryal Pus10 genes share a conserved catalytic domain with archaeal Pus10 genes. Pus10 is found in earlier evolutionary branches of fungi (such as chytrid Batrachochytrium) but is absent in all dikaryon fungi surveyed (Ascomycetes and Basidiomycetes). Orthologs of Pus10, TrmA, and TruB are present in all the animals, plants, and protozoa surveyed. This indicates that the common eukaryotic ancestor possesses all the three genes. Pus10 exists as a single copy gene in all the surveyed genomes despite ancestral whole genome duplications has occurred. This indicates a possible deleterious gene dosage effect. Functional redundancy results in gene loss or neofunctionalization in different evolutionary lineages | Arabidopsis thaliana |
| 5.4.99.B22 | evolution | eukaryal Pus10 genes share a conserved catalytic domain with archaeal Pus10 genes. Pus10 is found in earlier evolutionary branches of fungi (such as chytrid Batrachochytrium) but is absent in all dikaryon fungi surveyed (Ascomycetes and Basidiomycetes). Orthologs of Pus10, TrmA, and TruB are present in all the animals, plants, and protozoa surveyed. This indicates that the common eukaryotic ancestor possesses all the three genes. Pus10 exists as a single copy gene in all the surveyed genomes despite ancestral whole genome duplications has occurred. This indicates a possible deleterious gene dosage effect. Functional redundancy results in gene loss or neofunctionalization in different evolutionary lineages | Pyrococcus furiosus |
| 5.4.99.B22 | metabolism | human Pus10 participates in apoptosis induced by the tumor necrosis factor-related apoptosis-inducing ligand | Homo sapiens |
| 5.4.99.25 | evolution | in archaea, pseudouridine (Psi) synthase Pus10 modifies uridine (U) to Psi at positions 54 and 55 of tRNA. Pus10 is not found in bacteria, where modifications at those two positions are carried out by TrmA (U54 to m5U54) and TruB (U55 to Psi55). Many eukaryotes have an apparent redundancy, their genomes contain orthologues of archaeal Pus10 and bacterial TrmA and TruB. Eukaryal Pus10 genes share a conserved catalytic domain with archaeal Pus10 genes. Pus10 is found in earlier evolutionary branches of fungi (such as chytrid Batrachochytrium) but is absent in all dikaryon fungi surveyed (Ascomycetes and Basidiomycetes). Examination of 116 archaeal and eukaryotic Pus10 protein sequences reveals that Pus10 exists as a single copy gene in all the surveyed genomes despite ancestral whole genome duplications had occurred. Functional redundancy result in gene loss or neofunctionalization in different evolutionary lineages. The enzyme is a member of the pseudouridine synthase superfamily with a similar three-dimensional structure and a conserved catalytic Asp. In the catalytic region, five amino acids (Asp275, Tyr339, Ile412, Lys413, Leu440 in Methanocalcoccus jannaschii) are conserved throughout all pseudouridine synthase families | Homo sapiens |
| 5.4.99.25 | evolution | in archaea, pseudouridine (Psi) synthase Pus10 modifies uridine (U) to Psi at positions 54 and 55 of tRNA. Pus10 is not found in bacteria, where modifications at those two positions are carried out by TrmA (U54 to m5U54) and TruB (U55 to Psi55). Many eukaryotes have an apparent redundancy, their genomes contain orthologues of archaeal Pus10 and bacterial TrmA and TruB. Eukaryal Pus10 genes share a conserved catalytic domain with archaeal Pus10 genes. Pus10 is found in earlier evolutionary branches of fungi (such as chytrid Batrachochytrium) but is absent in all dikaryon fungi surveyed (Ascomycetes and Basidiomycetes). Examination of 116 archaeal and eukaryotic Pus10 protein sequences reveals that Pus10 exists as a single copy gene in all the surveyed genomes despite ancestral whole genome duplications had occurred. Functional redundancy result in gene loss or neofunctionalization in different evolutionary lineages. The enzyme is a member of the pseudouridine synthase superfamily with a similar three-dimensional structure and a conserved catalytic Asp. In the catalytic region, five amino acids (Asp275, Tyr339, Ile412, Lys413, Leu440 in Methanocalcoccus jannaschii) are conserved throughout all pseudouridine synthase families | Methanocaldococcus jannaschii |
| 5.4.99.B25 | evolution | in archaea, pseudouridine (Psi) synthase Pus10 modifies uridine (U) to Psi at positions 54 and 55 of tRNA. Pus10 is not found in bacteria, where modifications at those two positions are carried out by TrmA (U54 to m5U54) and TruB (U55 to Psi55). Many eukaryotes have an apparent redundancy, their genomes contain orthologues of archaeal Pus10 and bacterial TrmA and TruB. Eukaryal Pus10 genes share a conserved catalytic domain with archaeal Pus10 genes. Pus10 is found in earlier evolutionary branches of fungi (such as chytrid Batrachochytrium) but is absent in all dikaryon fungi surveyed (Ascomycetes and Basidiomycetes). Examination of 116 archaeal and eukaryotic Pus10 protein sequences reveals that Pus10 exists as a single copy gene in all the surveyed genomes despite ancestral whole genome duplications had occurred. Functional redundancy result in gene loss or neofunctionalization in different evolutionary lineages. The enzyme is a member of the pseudouridine synthase superfamily with a similar three-dimensional structure and a conserved catalytic Asp | Methanocaldococcus jannaschii |
| 5.4.99.B25 | evolution | in archaea, pseudouridine (Psi) synthase Pus10 modifies uridine (U) to Psi at positions 54 and 55 of tRNA. Pus10 is not found in bacteria, where modifications at those two positions are carried out by TrmA (U54 to m5U54) and TruB (U55 to Psi55). Many eukaryotes have an apparent redundancy, their genomes contain orthologues of archaeal Pus10 and bacterial TrmA and TruB. Eukaryal Pus10 genes share a conserved catalytic domain with archaeal Pus10 genes. Pus10 is found in earlier evolutionary branches of fungi (such as chytrid Batrachochytrium) but is absent in all dikaryon fungi surveyed (Ascomycetes and Basidiomycetes). Examination of 116 archaeal and eukaryotic Pus10 protein sequences reveals that Pus10 exists as a single copy gene in all the surveyed genomes despite ancestral whole genome duplications had occurred. Functional redundancy result in gene loss or neofunctionalization in different evolutionary lineages. The enzyme is a member of the pseudouridine synthase superfamily with a similar three-dimensional structure and a conserved catalytic Asp. In the catalytic region, five amino acids are conserved throughout all pseudouridine synthase families | Homo sapiens |
| 5.4.99.25 | additional information | homology modeling and structural superimposition using the crystal structure of Homo sapiens enzyme Pus10, PDB ID 2V9K, as a template, overview | Methanocaldococcus jannaschii |
| 5.4.99.25 | additional information | the human enzyme crystal structure, PDB ID 2V9K, is modelled onto the structure of Methanocalcoccus jannaschii | Homo sapiens |
| 5.4.99.B25 | additional information | homology modeling and structural superimposition using the crystal structure of Homo sapiens enzyme Pus10, PDB ID 2V9K, as a template, overview. In the catalytic region, five amino acids (Asp275, Tyr339, Ile412, Lys413, Leu440 in Methanocalcoccus jannaschii) are conserved throughout all pseudouridine synthase families | Methanocaldococcus jannaschii |
| 5.4.99.B25 | additional information | the human enzyme crystal structure, PDB ID 2V9K, is modelled onto the structure of Methanocalcoccus jannaschii | Homo sapiens |
| 5.4.99.25 | physiological function | human Pus10 participates in apoptosis induced by the tumor necrosis factor-related apoptosis-inducing ligand | Homo sapiens |
| 5.4.99.B25 | physiological function | human Pus10 participates in apoptosis induced by the tumor necrosis factor-related apoptosis-inducing ligand | Homo sapiens |
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