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Literature summary extracted from

  • Fucinos, P.; Pastrana, L.; Sanroman, A.; Longo, M.; Hermoso, J.; Rua, M.
    An esterase from Thermus thermophilus HB27 with hyper-thermoalkalophilic properties Purification, characterisation and structural modelling (2011), J. Mol. Catal. B, 70, 127-137 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.1.1 biotechnology due to its high thermal stability the enzyme is a very attractive enzyme for biotechnological purposes Thermus thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.1 membrane membrane-associated enzyme Thermus thermophilus 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.1 34000
-
SDS-PAGE Thermus thermophilus
3.1.1.1 36445
-
calculated from sequence Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Thermus thermophilus Q72J75
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1
-
Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 317.2
-
65°C, pH 8.0 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-nitrophenyl decanoate + H2O preference for medium chain-length esters Thermus thermophilus 4-nitrophenol + decanoate
-
?
3.1.1.1 4-nitrophenyl dodecanoate + H2O preference for medium chain-length esters Thermus thermophilus 4-nitrophenol + dodecanoate
-
?
3.1.1.1 4-nitrophenyl tetradecanoate + H2O preference for medium chain-length esters Thermus thermophilus 4-nitrophenol + tetradecanoate
-
?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 monomer 1 * 34000, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 E34Tt
-
Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 80
-
above Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.1 85
-
half-life: 135 min Thermus thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.1 Thermus thermophilus calculated from sequence
-
8.37