Literature summary extracted from

Li, C.Y.; Zhang, D.; Chen, X.L.; Wang, P.; Shi, W.L.; Li, P.Y.; Zhang, X.Y.; Qin, Q.L.; Todd, J.D.; Zhang, Y.Z.
Mechanistic insights into dimethylsulfoniopropionate lyase DddY, a new member of the cupin superfamily (2017), J. Mol. Biol., 429, 3850-3862 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.4.1.3	expression in Escherichia coli	Acinetobacter bereziniae

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
4.4.1.3	to 1.5 A resolution. DddY contains a cap domain and a catalytic domain with a Zn2+ bound at its active site. Residues His265, Glu269, His338 and the acetate molecule participate in coordinating Zn2+. The DddY catalytic domain adopts a typical beta-barrel fold and contains two conserved cupin motifs. In the catalytic mechanism, residue Tyr271 acts as a general base to attack DMSP	Acinetobacter bereziniae

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.4.1.3	5	-	beta-dimethylsulfoniopropanoate	pH 8.0, 60°C	Acinetobacter bereziniae

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
4.4.1.3	Zinc	Zn2+ is bound at the active site	Acinetobacter bereziniae

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.4.1.3	Acinetobacter bereziniae	N8X9V6	-	-
4.4.1.3	Acinetobacter bereziniae NIPH 3	N8X9V6	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.4.1.3	beta-dimethylsulfoniopropanoate	-	Acinetobacter bereziniae	dimethylsulfide + acrylate	-	?
4.4.1.3	beta-dimethylsulfoniopropanoate	-	Acinetobacter bereziniae NIPH 3	dimethylsulfide + acrylate	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
4.4.1.3	Dddy	-	Acinetobacter bereziniae
4.4.1.3	F963_02809	-	Acinetobacter bereziniae

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
4.4.1.3	60	-	-	Acinetobacter bereziniae

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.4.1.3	8300	-	beta-dimethylsulfoniopropanoate	pH 8.0, 60°C	Acinetobacter bereziniae

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.4.1.3	8	-	-	Acinetobacter bereziniae

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Release 2023.1 (January 2023)