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Literature summary extracted from

  • Wu, X.; Eiteman, M.A.
    Synthesis of citramalic acid from glycerol by metabolically engineered Escherichia coli (2017), J. Ind. Microbiol. Biotechnol., 44, 1483-1490 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.3.21 synthesis synthesis of citramalic acid from glycerol by metabolically engineered Escherichia coli Methanocaldococcus jannaschii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.21 expressed in Escherichia coli MG1655 cells Methanocaldococcus jannaschii
2.3.3.21 the modified citramalate synthase gene cimA from Methanococcus jannaschii is expressed in Escherichia coli. Gene knockouts and fermentation optimization improve citramalate production from glycerol and also reduce acetate accumulation. Near elimination of acetate formation necessitates deletions in genes for both pathways associated with acetate formation: ackA coding acetate kinase, pta coding phosphotransacetylase, and poxB coding pyruvate oxidase Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.21 acetyl-CoA + pyruvate + H2O Methanocaldococcus jannaschii
-
 CoA + citramalate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.21 Methanocaldococcus jannaschii
-
-
-
2.3.3.21 Methanocaldococcus jannaschii Q58787
-
-
2.3.3.21 Methanocaldococcus jannaschii DSM 2661 Q58787
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.21 acetate + pyruvate
-
 Methanocaldococcus jannaschii citramalate
-
 ?
2.3.3.21 acetate + pyruvate
-
 Methanocaldococcus jannaschii DSM 2661 citramalate
-
 ?
2.3.3.21 acetyl-CoA + pyruvate + H2O
-
 Methanocaldococcus jannaschii CoA + citramalate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.3.21 CimA
-
 Methanocaldococcus jannaschii
2.3.3.21 citramalate synthase
-
 Methanocaldococcus jannaschii