EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.B41 | expressed in Escherichia coli | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B41 | additional information | - |
4-nitrophenyl beta-D-glucopyranoside | Km-values decrease with an increase in temperature, whereas the kcat values increase, so the catalytic efficiency (kcat/Km) increases 70fold between 40°C and 80°C | Thermus thermophilus | |
3.2.1.B41 | 0.08 | - |
4-nitrophenyl beta-D-fucopyranoside | 80°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 0.16 | - |
4-nitrophenyl beta-D-glucopyranoside | 80°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 0.28 | - |
4-nitrophenyl beta-D-glucopyranoside | 70°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 0.39 | - |
4-nitrophenyl beta-D-glucopyranoside | 60°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 0.83 | - |
4-nitrophenyl beta-D-glucopyranoside | 50°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 1.74 | - |
4-nitrophenyl beta-D-galactopyranoside | 80°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 3.76 | - |
4-nitrophenyl beta-D-glucopyranoside | 40°C, pH 8.5 | Thermus thermophilus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.B41 | extracellular | - |
Thermus thermophilus | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.B41 | 45000 | - |
SDS-PAGE | Thermus thermophilus |
3.2.1.B41 | 48700 | - |
calculated from sequence | Thermus thermophilus |
3.2.1.B41 | 50000 | - |
gel filtration | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.B41 | Thermus thermophilus | A8WAC9 | - |
- |
3.2.1.B41 | Thermus thermophilus HJ6 | A8WAC9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.B41 | - |
Thermus thermophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.B41 | 4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Thermus thermophilus | 4-nitrophenol + D-fucose | - |
? | |
3.2.1.B41 | 4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Thermus thermophilus HJ6 | 4-nitrophenol + D-fucose | - |
? | |
3.2.1.B41 | 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermus thermophilus | 4-nitrophenol + D-galactose | - |
? | |
3.2.1.B41 | 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Thermus thermophilus HJ6 | 4-nitrophenol + D-galactose | - |
? | |
3.2.1.B41 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermus thermophilus | 4-nitrophenol + D-glucose | - |
? | |
3.2.1.B41 | 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Thermus thermophilus HJ6 | 4-nitrophenol + D-glucose | - |
? | |
3.2.1.B41 | additional information | the enzyme has transglycosylation activity at high temperature and that various transfer products were formed in the reaction with lactose or cellobiose | Thermus thermophilus | ? | - |
? | |
3.2.1.B41 | additional information | the enzyme has transglycosylation activity at high temperature and that various transfer products were formed in the reaction with lactose or cellobiose | Thermus thermophilus HJ6 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.B41 | monomer | 1 * 45000, SDS-PAGE | Thermus thermophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.B41 | TtbetaGly | - |
Thermus thermophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.B41 | 90 | - |
- |
Thermus thermophilus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.B41 | 70 | 105 | 70°C: about 55% of maximal activity, 105°C: about 60% of maximal activity | Thermus thermophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.B41 | 95 | - |
half-life: 30 min | Thermus thermophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B41 | additional information | - |
4-nitrophenyl beta-D-glucopyranoside | Km-values decrease with an increase in temperature, whereas the kcat values increase, so the catalytic efficiency (kcat/Km) increases 70fold between 40°C and 80°C | Thermus thermophilus | |
3.2.1.B41 | 3.1 | - |
4-nitrophenyl beta-D-glucopyranoside | 40°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 3.17 | - |
4-nitrophenyl beta-D-glucopyranoside | 50°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 4.78 | - |
4-nitrophenyl beta-D-glucopyranoside | 60°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 7.56 | - |
4-nitrophenyl beta-D-glucopyranoside | 70°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 8.94 | - |
4-nitrophenyl beta-D-fucopyranoside | 80°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 9.24 | - |
4-nitrophenyl beta-D-glucopyranoside | 80°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 9.64 | - |
4-nitrophenyl beta-D-galactopyranoside | 80°C, pH 8.5 | Thermus thermophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.B41 | 8.5 | - |
- |
Thermus thermophilus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.B41 | 8 | 10 | pH 8.0: about 40% of maximal activity, pH 10.0: about 70% of maximal activity | Thermus thermophilus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.B41 | Thermus thermophilus | calculated from sequence | - |
5.6 |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.B41 | additional information | - |
4-nitrophenyl beta-D-glucopyranoside | Km-values decrease with an increase in temperature, whereas the kcat values increase, so the catalytic efficiency (kcat/Km) increases 70fold between 40°C and 80°C | Thermus thermophilus | |
3.2.1.B41 | 0.82 | - |
4-nitrophenyl beta-D-glucopyranoside | 40°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 3.83 | - |
4-nitrophenyl beta-D-glucopyranoside | 50°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 5.56 | - |
4-nitrophenyl beta-D-galactopyranoside | 80°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 12.22 | - |
4-nitrophenyl beta-D-glucopyranoside | 60°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 27.45 | - |
4-nitrophenyl beta-D-glucopyranoside | 70°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 57.12 | - |
4-nitrophenyl beta-D-glucopyranoside | 80°C, pH 8.5 | Thermus thermophilus | |
3.2.1.B41 | 104.83 | - |
4-nitrophenyl beta-D-fucopyranoside | 80°C, pH 8.5 | Thermus thermophilus |