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Literature summary extracted from

  • Gu, N.; Kim, J.; Kim, H.; You, D.; Kim, H.; Jeon, S.
    Gene cloning and enzymatic properties of hyperthermostable beta-glycosidase from Thermus thermophilus HJ6 (2009), J. Biosci. Bioeng., 107, 21-26 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B41 expressed in Escherichia coli Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B41 additional information
-
4-nitrophenyl beta-D-glucopyranoside Km-values decrease with an increase in temperature, whereas the kcat values increase, so the catalytic efficiency (kcat/Km) increases 70fold between 40°C and 80°C Thermus thermophilus
3.2.1.B41 0.08
-
4-nitrophenyl beta-D-fucopyranoside 80°C, pH 8.5 Thermus thermophilus
3.2.1.B41 0.16
-
4-nitrophenyl beta-D-glucopyranoside 80°C, pH 8.5 Thermus thermophilus
3.2.1.B41 0.28
-
4-nitrophenyl beta-D-glucopyranoside 70°C, pH 8.5 Thermus thermophilus
3.2.1.B41 0.39
-
4-nitrophenyl beta-D-glucopyranoside 60°C, pH 8.5 Thermus thermophilus
3.2.1.B41 0.83
-
4-nitrophenyl beta-D-glucopyranoside 50°C, pH 8.5 Thermus thermophilus
3.2.1.B41 1.74
-
4-nitrophenyl beta-D-galactopyranoside 80°C, pH 8.5 Thermus thermophilus
3.2.1.B41 3.76
-
4-nitrophenyl beta-D-glucopyranoside 40°C, pH 8.5 Thermus thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.B41 extracellular
-
Thermus thermophilus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.B41 45000
-
SDS-PAGE Thermus thermophilus
3.2.1.B41 48700
-
calculated from sequence Thermus thermophilus
3.2.1.B41 50000
-
gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B41 Thermus thermophilus A8WAC9
-
-
3.2.1.B41 Thermus thermophilus HJ6 A8WAC9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.B41
-
Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B41 4-nitrophenyl beta-D-fucopyranoside + H2O
-
Thermus thermophilus 4-nitrophenol + D-fucose
-
?
3.2.1.B41 4-nitrophenyl beta-D-fucopyranoside + H2O
-
Thermus thermophilus HJ6 4-nitrophenol + D-fucose
-
?
3.2.1.B41 4-nitrophenyl beta-D-galactopyranoside + H2O
-
Thermus thermophilus 4-nitrophenol + D-galactose
-
?
3.2.1.B41 4-nitrophenyl beta-D-galactopyranoside + H2O
-
Thermus thermophilus HJ6 4-nitrophenol + D-galactose
-
?
3.2.1.B41 4-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermus thermophilus 4-nitrophenol + D-glucose
-
?
3.2.1.B41 4-nitrophenyl beta-D-glucopyranoside + H2O
-
Thermus thermophilus HJ6 4-nitrophenol + D-glucose
-
?
3.2.1.B41 additional information the enzyme has transglycosylation activity at high temperature and that various transfer products were formed in the reaction with lactose or cellobiose Thermus thermophilus ?
-
?
3.2.1.B41 additional information the enzyme has transglycosylation activity at high temperature and that various transfer products were formed in the reaction with lactose or cellobiose Thermus thermophilus HJ6 ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.B41 monomer 1 * 45000, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B41 TtbetaGly
-
Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B41 90
-
-
Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.B41 70 105 70°C: about 55% of maximal activity, 105°C: about 60% of maximal activity Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B41 95
-
half-life: 30 min Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B41 additional information
-
4-nitrophenyl beta-D-glucopyranoside Km-values decrease with an increase in temperature, whereas the kcat values increase, so the catalytic efficiency (kcat/Km) increases 70fold between 40°C and 80°C Thermus thermophilus
3.2.1.B41 3.1
-
4-nitrophenyl beta-D-glucopyranoside 40°C, pH 8.5 Thermus thermophilus
3.2.1.B41 3.17
-
4-nitrophenyl beta-D-glucopyranoside 50°C, pH 8.5 Thermus thermophilus
3.2.1.B41 4.78
-
4-nitrophenyl beta-D-glucopyranoside 60°C, pH 8.5 Thermus thermophilus
3.2.1.B41 7.56
-
4-nitrophenyl beta-D-glucopyranoside 70°C, pH 8.5 Thermus thermophilus
3.2.1.B41 8.94
-
4-nitrophenyl beta-D-fucopyranoside 80°C, pH 8.5 Thermus thermophilus
3.2.1.B41 9.24
-
4-nitrophenyl beta-D-glucopyranoside 80°C, pH 8.5 Thermus thermophilus
3.2.1.B41 9.64
-
4-nitrophenyl beta-D-galactopyranoside 80°C, pH 8.5 Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B41 8.5
-
-
Thermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.B41 8 10 pH 8.0: about 40% of maximal activity, pH 10.0: about 70% of maximal activity Thermus thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.B41 Thermus thermophilus calculated from sequence
-
5.6

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.B41 additional information
-
4-nitrophenyl beta-D-glucopyranoside Km-values decrease with an increase in temperature, whereas the kcat values increase, so the catalytic efficiency (kcat/Km) increases 70fold between 40°C and 80°C Thermus thermophilus
3.2.1.B41 0.82
-
4-nitrophenyl beta-D-glucopyranoside 40°C, pH 8.5 Thermus thermophilus
3.2.1.B41 3.83
-
4-nitrophenyl beta-D-glucopyranoside 50°C, pH 8.5 Thermus thermophilus
3.2.1.B41 5.56
-
4-nitrophenyl beta-D-galactopyranoside 80°C, pH 8.5 Thermus thermophilus
3.2.1.B41 12.22
-
4-nitrophenyl beta-D-glucopyranoside 60°C, pH 8.5 Thermus thermophilus
3.2.1.B41 27.45
-
4-nitrophenyl beta-D-glucopyranoside 70°C, pH 8.5 Thermus thermophilus
3.2.1.B41 57.12
-
4-nitrophenyl beta-D-glucopyranoside 80°C, pH 8.5 Thermus thermophilus
3.2.1.B41 104.83
-
4-nitrophenyl beta-D-fucopyranoside 80°C, pH 8.5 Thermus thermophilus