Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Smith, A.M.; Brown, W.C.; Harms, E.; Smith, J.L.
    Crystal structures capture three states in the catalytic cycle of a pyridoxal phosphate (PLP) synthase (2015), J. Biol. Chem., 290, 5226-5239 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.6 expression in Escherichia coli strain BL21(DE3) Geobacillus kaustophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.3.6 sitting drop vapor diffusion, to investigate the mechanism of the synthase subunit, crystal structures are obtained for three intermediate states of the Geobacillus stearothermophilus intact PLPS or its synthase subunit. The structures capture the synthase active site at three distinct steps in its complicated catalytic cycle, provide insights into the elusive mechanism, and illustrate the coordinated motions within the synthase subunit that separate the catalytic states Geobacillus kaustophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.3.6 0.01
-
 D-ribose 5-phosphate pH 8.5, 37°C Geobacillus kaustophilus
4.3.3.6 0.6
-
 L-glutamine pH 8.5, 37°C, glutaminase activity Geobacillus kaustophilus
4.3.3.6 1.06
-
 D-glyceraldehyde 3-phosphate pH 8.5, 37°C Geobacillus kaustophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.3.6 D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine Geobacillus kaustophilus
-
 pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.6 Geobacillus kaustophilus Q5L3Y2 AND Q5L3Y1 Q5L3Y2: subunit PdxS, Q5L3Y1: subunit PdxT
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.6
-
 Geobacillus kaustophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.6 D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine
-
 Geobacillus kaustophilus pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate
-
 ?
4.3.3.6 D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + NH3
-
 Geobacillus kaustophilus pyridoxal 5'-phosphate + 4 H2O + phosphate
-
 ?
4.3.3.6 L-glutamine + H2O
-
 Geobacillus kaustophilus L-glutamate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.3.6 oligomer the intact enzyme includes 12 synthase and 12 glutaminase subunits Geobacillus kaustophilus

Synonyms

EC Number Synonyms Comment Organism
4.3.3.6 PLP synthase
-
 Geobacillus kaustophilus
4.3.3.6 PLPS
-
 Geobacillus kaustophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.3.6 0.00017
-
 D-glyceraldehyde 3-phosphate pH 8.5, 37°C Geobacillus kaustophilus
4.3.3.6 0.00017
-
 D-ribose 5-phosphate pH 8.5, 37°C Geobacillus kaustophilus
4.3.3.6 0.001
-
 L-glutamine pH 8.5, 37°C, glutaminase activity Geobacillus kaustophilus