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Literature summary extracted from
- The yeast cadmium factor protein (YCF1) is a vacuolar glutathione S-conjugate pump (1996), J. Biol. Chem., 271, 6509-6517 .
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.6.2.3 | glutathione disulfide | - |
Saccharomyces cerevisiae |  |
| 7.6.2.3 | vanadate | - |
Saccharomyces cerevisiae | |
| 7.6.2.3 | verapamil | - |
Saccharomyces cerevisiae | |
| 7.6.2.3 | vinblastine | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.6.2.3 | 0.0141 | - |
S-(2,4-dinitrophenyl)glutathione[side 1] | at pH 8.0 and 25°C | Saccharomyces cerevisiae | |
| 7.6.2.3 | 0.0865 | - |
ATP | at pH 8.0 and 25°C | Saccharomyces cerevisiae | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.6.2.3 | vacuolar membrane | - |
Saccharomyces cerevisiae | 5774 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.6.2.3 | Mg2+ | required in form of MgATP | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.6.2.3 | ATP + H2O + glutathione-S-conjugate[side 1] | Saccharomyces cerevisiae | - |
ADP + phosphate + glutathione-S-conjugate[side 2] | - |
? | |
| 7.6.2.3 | ATP + H2O + glutathione-S-conjugate[side 1] | Saccharomyces cerevisiae DTY165 | - |
ADP + phosphate + glutathione-S-conjugate[side 2] | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.6.2.3 | Saccharomyces cerevisiae | P39109 | - |
- |
| 7.6.2.3 | Saccharomyces cerevisiae DTY165 | P39109 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.6.2.3 | ATP + H2O + glutathione-S-conjugate[side 1] | - |
Saccharomyces cerevisiae | ADP + phosphate + glutathione-S-conjugate[side 2] | - |
? | |
| 7.6.2.3 | ATP + H2O + glutathione-S-conjugate[side 1] | - |
Saccharomyces cerevisiae DTY165 | ADP + phosphate + glutathione-S-conjugate[side 2] | - |
? | |
| 7.6.2.3 | ATP + H2O + S-(2,4-dinitrophenyl)glutathione[side 1] | - |
Saccharomyces cerevisiae | ADP + phosphate + S-(2,4-dinitrophenyl)glutathione[side 2] | - |
? | |
| 7.6.2.3 | ATP + H2O + S-(2,4-dinitrophenyl)glutathione[side 1] | - |
Saccharomyces cerevisiae DTY165 | ADP + phosphate + S-(2,4-dinitrophenyl)glutathione[side 2] | - |
? | |
| 7.6.2.3 | ATP + H2O + S-(azidophenacyl)glutathione[side 1] | - |
Saccharomyces cerevisiae | ADP + phosphate + S-(azidophenacyl)glutathione[side 2] | - |
? | |
| 7.6.2.3 | ATP + H2O + S-(azidophenacyl)glutathione[side 1] | - |
Saccharomyces cerevisiae DTY165 | ADP + phosphate + S-(azidophenacyl)glutathione[side 2] | - |
? | |
| 7.6.2.3 | ATP + H2O + S-(metolachlor)glutathione[side 1] | - |
Saccharomyces cerevisiae | ADP + phosphate + S-(metolachlor)glutathione[side 2] | - |
? | |
| 7.6.2.3 | ATP + H2O + S-(metolachlor)glutathione[side 1] | - |
Saccharomyces cerevisiae DTY165 | ADP + phosphate + S-(metolachlor)glutathione[side 2] | - |
? | |
| 7.6.2.3 | ATP + H2O + S-(monochlorobimane)glutathione[side 1] | - |
Saccharomyces cerevisiae | ADP + phosphate + S-(monochlorobimane)glutathione[side 2] | - |
? | |
| 7.6.2.3 | ATP + H2O + S-(monochlorobimane)glutathione[side 1] | - |
Saccharomyces cerevisiae DTY165 | ADP + phosphate + S-(monochlorobimane)glutathione[side 2] | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 7.6.2.3 | ? | x * 156200, SDS-PAGE | Saccharomyces cerevisiae |
| 7.6.2.3 | ? | x * 172000, calculated from amino acid sequence | Saccharomyces cerevisiae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.6.2.3 | cadmium factor protein | - |
Saccharomyces cerevisiae |
| 7.6.2.3 | glutathione S-conjugate pump | - |
Saccharomyces cerevisiae |
| 7.6.2.3 | vacuolar glutathione S-conjugate transporter | - |
Saccharomyces cerevisiae |
| 7.6.2.3 | Ycf1 | - |
Saccharomyces cerevisiae |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 7.6.2.3 | 0.02026 | - |
at pH 8.0 and 25°C | Saccharomyces cerevisiae | verapamil | |
| 7.6.2.3 | 0.0888 | - |
at pH 8.0 and 25°C | Saccharomyces cerevisiae | vinblastine | |
| 7.6.2.3 | 0.1791 | - |
at pH 8.0 and 25°C | Saccharomyces cerevisiae | vanadate | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 7.6.2.3 | physiological function | the enzyme is required for increased resistance to the toxic effects of the exogenous glutathione S-conjugate precursor, 1-chloro-2,4-dinitrobenzene, as well as cadmium | Saccharomyces cerevisiae |
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