EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.2.3 | expression in Escherichia coli | Shewanella sp. YH1 |
4.2.2.11 | expression in Escherichia coli | Shewanella sp. YH1 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.3 | Al3+ | 1 mM, 59% residual activity | Shewanella sp. YH1 | |
4.2.2.3 | Ba2+ | 1 mM, 13% residual activity | Shewanella sp. YH1 | |
4.2.2.3 | Ca2+ | 1 mM, 36% residual activity | Shewanella sp. YH1 | |
4.2.2.3 | Co2+ | 1 mM, 12% residual activity | Shewanella sp. YH1 | |
4.2.2.3 | Cu2+ | 1 mM, 8% residual activity | Shewanella sp. YH1 | |
4.2.2.3 | EDTA | 1 mM, 8% residual activity | Shewanella sp. YH1 | |
4.2.2.3 | Fe3+ | 1 mM, 60% residual activity | Shewanella sp. YH1 | |
4.2.2.3 | Mn2+ | 1 mM, 8% residual activity | Shewanella sp. YH1 | |
4.2.2.3 | additional information | enzymatic activity is about two times higher in phosphate buffer than in Tris buffer | Shewanella sp. YH1 | |
4.2.2.3 | N-bromosuccinimide | 1 mM, no residual activity | Shewanella sp. YH1 | |
4.2.2.3 | Ni2+ | 1 mM, 20% residual activity | Shewanella sp. YH1 | |
4.2.2.11 | Al3+ | 1 mM, 59% residual activity | Shewanella sp. YH1 | |
4.2.2.11 | Ba2+ | 1 mM, 13% residual activity | Shewanella sp. YH1 | |
4.2.2.11 | Ca2+ | 1 mM, 36% residual activity | Shewanella sp. YH1 | |
4.2.2.11 | Co2+ | 1 mM, 12% residual activity | Shewanella sp. YH1 | |
4.2.2.11 | Cu2+ | 1 mM, 8% residual activity | Shewanella sp. YH1 | |
4.2.2.11 | EDTA | 1 mM, 8% residual activity | Shewanella sp. YH1 | |
4.2.2.11 | Fe3+ | 1 mM, 60% residual activity | Shewanella sp. YH1 | |
4.2.2.11 | Mn3+ | 1 mM, 8% residual activity | Shewanella sp. YH1 | |
4.2.2.11 | additional information | enzymatic activity is about two times higher in phosphate buffer than in Tris buffer | Shewanella sp. YH1 | |
4.2.2.11 | N-bromosuccinimide | 1 mM, no residual activity | Shewanella sp. YH1 | |
4.2.2.11 | Ni2+ | 1 mM, 20% residual activity | Shewanella sp. YH1 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.2.3 | Shewanella sp. YH1 | - |
- |
- |
4.2.2.11 | Shewanella sp. YH1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.2.3 | native and recombinant protein | Shewanella sp. YH1 |
4.2.2.11 | native and recombinant protein | Shewanella sp. YH1 |
EC Number | Renatured (Comment) | Organism |
---|---|---|
4.2.2.3 | enzyme is not thermotolerant, but can refold to its active form following an almost complete denaturation at approximately 60°C | Shewanella sp. YH1 |
4.2.2.11 | enzyme is not thermotolerant, but can refold to its active form following an almost complete denaturation at approximately 60°C | Shewanella sp. YH1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.3 | alginate | - |
Shewanella sp. YH1 | unsaturated algino-oligosaccharides | - |
? | |
4.2.2.3 | alginate | about 50% of the activity with poly(alpha-1,4-L-guluronate) | Shewanella sp. YH1 | unsaturated algino-oligosaccharides | - |
? | |
4.2.2.3 | poly(alpha-1,4-L-guluronate) | reaction of EC 4.2.2.11 | Shewanella sp. YH1 | 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? | |
4.2.2.3 | poly(beta-1,4-D-mannuronate) | about 20% of the activity with poly(alpha-1,4-L-guluronate) | Shewanella sp. YH1 | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) | - |
? | |
4.2.2.3 | poly(beta-1,4-D-mannuronate/alpha-1,4-L-guluronate) | about 30% of the activity with poly(alpha-1,4-L-guluronate) | Shewanella sp. YH1 | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) + 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? | |
4.2.2.11 | alginate | - |
Shewanella sp. YH1 | unsaturated algino-oligosaccharides | - |
? | |
4.2.2.11 | alginate | about 50% of the activity with poly(alpha-(1,4)-L-guluronate) | Shewanella sp. YH1 | unsaturated algino-oligosaccharides | - |
? | |
4.2.2.11 | poly(alpha-(1,4)-L-guluronate) | - |
Shewanella sp. YH1 | 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? | |
4.2.2.11 | poly(beta-(1,4)-D-mannuronate) | reaction of EC 4.2.2.3, about 20% of the activity with poly(alpha-(1,4)-L-guluronate) | Shewanella sp. YH1 | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) | - |
? | |
4.2.2.11 | poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate) | about 30% of the activity with poly(alpha-(1,4)-L-guluronate) | Shewanella sp. YH1 | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) + 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.2.3 | ? | x * 35000, SDS-PAGE, x * 36376, calculated from sequence | Shewanella sp. YH1 |
4.2.2.11 | ? | x * 35000, SDS-PAGE, x * 36376, calculated from sequence | Shewanella sp. YH1 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 45 | - |
- |
Shewanella sp. YH1 |
4.2.2.11 | 45 | - |
- |
Shewanella sp. YH1 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 8 | - |
- |
Shewanella sp. YH1 |
4.2.2.11 | 8 | - |
- |
Shewanella sp. YH1 |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 7 | 9 | at least 70% of maximum activity | Shewanella sp. YH1 |
4.2.2.11 | 7 | 9 | at least 70% of maximum activity | Shewanella sp. YH1 |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.2.2.3 | Shewanella sp. YH1 | calculated from sequence | - |
6 |
4.2.2.11 | Shewanella sp. YH1 | calculated from sequence | - |
6 |