Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yagi, H.; Fujise, A.; Itabashi, N.; Ohshiro, T.
    Characterization of a novel endo-type alginate lyase derived from Shewanella sp. YH1 (2018), J. Biochem., 163, 341-350 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.3 expression in Escherichia coli Shewanella sp. YH1
4.2.2.11 expression in Escherichia coli Shewanella sp. YH1

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.3 Al3+ 1 mM, 59% residual activity Shewanella sp. YH1
4.2.2.3 Ba2+ 1 mM, 13% residual activity Shewanella sp. YH1
4.2.2.3 Ca2+ 1 mM, 36% residual activity Shewanella sp. YH1
4.2.2.3 Co2+ 1 mM, 12% residual activity Shewanella sp. YH1
4.2.2.3 Cu2+ 1 mM, 8% residual activity Shewanella sp. YH1
4.2.2.3 EDTA 1 mM, 8% residual activity Shewanella sp. YH1
4.2.2.3 Fe3+ 1 mM, 60% residual activity Shewanella sp. YH1
4.2.2.3 Mn2+ 1 mM, 8% residual activity Shewanella sp. YH1
4.2.2.3 additional information enzymatic activity is about two times higher in phosphate buffer than in Tris buffer Shewanella sp. YH1
4.2.2.3 N-bromosuccinimide 1 mM, no residual activity Shewanella sp. YH1
4.2.2.3 Ni2+ 1 mM, 20% residual activity Shewanella sp. YH1
4.2.2.11 Al3+ 1 mM, 59% residual activity Shewanella sp. YH1
4.2.2.11 Ba2+ 1 mM, 13% residual activity Shewanella sp. YH1
4.2.2.11 Ca2+ 1 mM, 36% residual activity Shewanella sp. YH1
4.2.2.11 Co2+ 1 mM, 12% residual activity Shewanella sp. YH1
4.2.2.11 Cu2+ 1 mM, 8% residual activity Shewanella sp. YH1
4.2.2.11 EDTA 1 mM, 8% residual activity Shewanella sp. YH1
4.2.2.11 Fe3+ 1 mM, 60% residual activity Shewanella sp. YH1
4.2.2.11 Mn3+ 1 mM, 8% residual activity Shewanella sp. YH1
4.2.2.11 additional information enzymatic activity is about two times higher in phosphate buffer than in Tris buffer Shewanella sp. YH1
4.2.2.11 N-bromosuccinimide 1 mM, no residual activity Shewanella sp. YH1
4.2.2.11 Ni2+ 1 mM, 20% residual activity Shewanella sp. YH1

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Shewanella sp. YH1
-
-
-
4.2.2.11 Shewanella sp. YH1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.3 native and recombinant protein Shewanella sp. YH1
4.2.2.11 native and recombinant protein Shewanella sp. YH1

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.2.2.3 enzyme is not thermotolerant, but can refold to its active form following an almost complete denaturation at approximately 60°C Shewanella sp. YH1
4.2.2.11 enzyme is not thermotolerant, but can refold to its active form following an almost complete denaturation at approximately 60°C Shewanella sp. YH1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate
-
Shewanella sp. YH1 unsaturated algino-oligosaccharides
-
?
4.2.2.3 alginate about 50% of the activity with poly(alpha-1,4-L-guluronate) Shewanella sp. YH1 unsaturated algino-oligosaccharides
-
?
4.2.2.3 poly(alpha-1,4-L-guluronate) reaction of EC 4.2.2.11 Shewanella sp. YH1 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
?
4.2.2.3 poly(beta-1,4-D-mannuronate) about 20% of the activity with poly(alpha-1,4-L-guluronate) Shewanella sp. YH1 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
?
4.2.2.3 poly(beta-1,4-D-mannuronate/alpha-1,4-L-guluronate) about 30% of the activity with poly(alpha-1,4-L-guluronate) Shewanella sp. YH1 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) + 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
?
4.2.2.11 alginate
-
Shewanella sp. YH1 unsaturated algino-oligosaccharides
-
?
4.2.2.11 alginate about 50% of the activity with poly(alpha-(1,4)-L-guluronate) Shewanella sp. YH1 unsaturated algino-oligosaccharides
-
?
4.2.2.11 poly(alpha-(1,4)-L-guluronate)
-
Shewanella sp. YH1 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
?
4.2.2.11 poly(beta-(1,4)-D-mannuronate) reaction of EC 4.2.2.3, about 20% of the activity with poly(alpha-(1,4)-L-guluronate) Shewanella sp. YH1 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
?
4.2.2.11 poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate) about 30% of the activity with poly(alpha-(1,4)-L-guluronate) Shewanella sp. YH1 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) + 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 ? x * 35000, SDS-PAGE, x * 36376, calculated from sequence Shewanella sp. YH1
4.2.2.11 ? x * 35000, SDS-PAGE, x * 36376, calculated from sequence Shewanella sp. YH1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 45
-
-
Shewanella sp. YH1
4.2.2.11 45
-
-
Shewanella sp. YH1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 8
-
-
Shewanella sp. YH1
4.2.2.11 8
-
-
Shewanella sp. YH1

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.3 7 9 at least 70% of maximum activity Shewanella sp. YH1
4.2.2.11 7 9 at least 70% of maximum activity Shewanella sp. YH1

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.3 Shewanella sp. YH1 calculated from sequence
-
6
4.2.2.11 Shewanella sp. YH1 calculated from sequence
-
6