EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.99.6 | overexpression in Escherichia coli | Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.99.6 | 0.46 | - |
D-alanine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 0.81 | - |
D-histidine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 1.07 | - |
D-phenylalanine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 1.65 | - |
D-serine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 1.78 | - |
D-tyrosine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 1.82 | - |
D-proline | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 2.47 | - |
D-threonine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 3.01 | - |
D-valine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 5.37 | - |
D-arginine | pH 8.7, 37°C | Pseudomonas aeruginosa |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.4.99.6 | cytoplasm | - |
Pseudomonas aeruginosa | 5737 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.99.6 | D-alanine + H2O + FAD | Pseudomonas aeruginosa | D-amino acid dehydrogenase activity of broad substrate specificity | pyruvate + NH3 + FADH2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.99.6 | Pseudomonas aeruginosa | Q9HTQ0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.99.6 | hexahistidine-tagged DadA | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.99.6 | D-alanine + H2O + FAD | D-amino acid dehydrogenase activity of broad substrate specificity | Pseudomonas aeruginosa | pyruvate + NH3 + FADH2 | - |
? | |
1.4.99.6 | D-alanine + H2O + FAD | the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity | Pseudomonas aeruginosa | pyruvate + NH3 + FADH2 | - |
? | |
1.4.99.6 | D-arginine + H2O + FAD | the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity | Pseudomonas aeruginosa | 5-guanidino-2-oxopentanoate + NH3 + FADH2 | - |
? | |
1.4.99.6 | D-histidine + H2O + FAD | the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity | Pseudomonas aeruginosa | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + FADH2 | - |
? | |
1.4.99.6 | D-phenylalanine + H2O + FAD | the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity | Pseudomonas aeruginosa | phenylpyruvate + NH3 + FADH2 | - |
? | |
1.4.99.6 | D-proline + H2O + FAD | the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity | Pseudomonas aeruginosa | 2-oxopentanoic acid + NH3 + FADH2 | - |
? | |
1.4.99.6 | D-serine + H2O + FAD | the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity | Pseudomonas aeruginosa | 3-hydroxy-2-oxopropanoate + NH3 + FADH2 | - |
? | |
1.4.99.6 | D-threonine + H2O + FAD | the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity | Pseudomonas aeruginosa | 3-hydroxy-2-oxobutanoate + NH3 + FADH2 | - |
? | |
1.4.99.6 | D-tyrosine + H2O + FAD | the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity | Pseudomonas aeruginosa | (4-hydroxyphenyl)pyruvate + NH3 + FADH2 | - |
? | |
1.4.99.6 | D-valine + H2O + FAD | the enzyme is an efficient D-amino acid dehydrogenase of broad substrate specificity | Pseudomonas aeruginosa | 2-oxopentanoate + NH3 + FADH2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.99.6 | D-amino acid dehydrogenase DadA | - |
Pseudomonas aeruginosa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.4.99.6 | 37 | - |
assay at | Pseudomonas aeruginosa |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.99.6 | 0.68 | - |
D-threonine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 0.74 | - |
D-alanine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 0.78 | - |
D-valine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 0.8 | - |
D-serine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 0.84 | - |
D-histidine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 0.97 | - |
D-proline | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 1.1 | - |
D-phenylalanine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 1.1 | - |
D-tyrosine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 1.27 | - |
D-arginine | pH 8.7, 37°C | Pseudomonas aeruginosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.4.99.6 | 8.7 | - |
assay at | Pseudomonas aeruginosa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.99.6 | FAD | - |
Pseudomonas aeruginosa |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.4.99.6 | Pseudomonas aeruginosa | highly up-regulated by L-Ala and D-Ala, followed by L-Val, L-Trp, L-Ser, L-Thr, L-Lys, Gly, L-Cys and several L-amino acids. Expression requires a transcriptional regulator, DadR | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.4.99.6 | malfunction | utilization of L-Ala, L-Trp, D-Ala, D-His, D-Phe, D-Ser, D-Thr, and D-Val as sole nitrogen sources is abolished in the dadA mutant | Pseudomonas aeruginosa |
1.4.99.6 | metabolism | D-amino acid dehydrogenase activity of broad substrate specificity | Pseudomonas aeruginosa |
1.4.99.6 | physiological function | involved in catabolism of several D-amino acids | Pseudomonas aeruginosa |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.99.6 | 0.236 | - |
D-arginine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 0.259 | - |
D-valine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 0.275 | - |
D-threonine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 0.485 | - |
D-serine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 0.533 | - |
D-proline | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 0.618 | - |
D-tyrosine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 1.03 | - |
D-phenylalanine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 1.04 | - |
D-histidine | pH 8.7, 37°C | Pseudomonas aeruginosa | |
1.4.99.6 | 1.61 | - |
D-alanine | pH 8.7, 37°C | Pseudomonas aeruginosa |