Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sankaran, K.; Gan, K.; Rash, B.; Qi, H.Y.; Wu, H.C.; Rick, P.D.
    Roles of histidine-103 and tyrosine-235 in the function of the prolipoprotein diacylglyceryl transferase of Escherichia coli (1997), J. Bacteriol., 179, 2944-2948 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.145 expressed in Escherichia coli SK634 cells Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.145 H103N inactive Escherichia coli
2.5.1.145 H196N the mutations result in an increase of in vitro activity (110% of wild type activity) Escherichia coli
2.5.1.145 H196Q the mutation results in a significant reduction of in vitro activity (50% of wild type activity) Escherichia coli
2.5.1.145 H196R the mutation results in a significant reduction of in vitro activity (50% of wild type activity) Escherichia coli
2.5.1.145 H2103Q inactive Escherichia coli
2.5.1.145 H289Q the mutation results in a reduction of in vitro activity (90% of wild type activity) Escherichia coli
2.5.1.145 H7Q the mutant shows wild type activity Escherichia coli
2.5.1.145 H7Q/H24Q/H103Q/H289Q inactive Escherichia coli
2.5.1.145 H7Q/H24Q/H196Q/H289Q the mutations result in a reduction of in vitro activity (40% of wild type activity) Escherichia coli
2.5.1.145 H7Q/H24Q/H289Q the mutations result in a reduction of in vitro activity (90% of wild type activity) Escherichia coli
2.5.1.145 H7Q/H289Q H7Q/H24Q/H289Q the mutations result in an increase of in vitro activity (110% of wild type activity) Escherichia coli
2.5.1.145 Y190F the mutant shows 110% activity compared to the wild type enzyme Escherichia coli
2.5.1.145 Y201F the mutant shows wild type activity Escherichia coli
2.5.1.145 Y235F inactive Escherichia coli
2.5.1.145 Y235S the mutant shows 60% activity compared to the wild type enzyme Escherichia coli
2.5.1.145 Y235T inactive Escherichia coli
2.5.1.145 Y26F the mutant shows 120% activity compared to the wild type enzyme Escherichia coli
2.5.1.145 Y282F the mutant shows 90% activity compared to the wild type enzyme Escherichia coli
2.5.1.145 Y62F the mutant shows 120% activity compared to the wild type enzyme Escherichia coli
2.5.1.145 Y76F the mutant shows 110% activity compared to the wild type enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.145 diethylpyrocarbonate
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.145 0.007
-
 L-1-phosphatidyl-sn-glycerol mutant enzyme H196Q, at pH 8.0 and 37°C Escherichia coli
2.5.1.145 0.01
-
 L-1-phosphatidyl-sn-glycerol wild type enzyme, at pH 8.0 and 37°C Escherichia coli
2.5.1.145 0.028
-
 MKATKLVLGAVILGSTLLAGCSSN mutant enzyme H196Q, at pH 8.0 and 37°C Escherichia coli
2.5.1.145 0.03
-
 MKATKLVLGAVILGSTLLAGCSSN wild type enzyme, at pH 8.0 and 37°C Escherichia coli
2.5.1.145 0.03
-
 L-1-phosphatidyl-sn-glycerol mutant enzyme Y235S, at pH 8.0 and 37°C Escherichia coli
2.5.1.145 0.067
-
 MKATKLVLGAVILGSTLLAGCSSN mutant enzyme Y235S, at pH 8.0 and 37°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.145 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.145 L-1-phosphatidyl-sn-glycerol + MKATKLVLGAVILGSTLLAGCSSN
-
 Escherichia coli sn-glycerol 1-phosphate + MKATKLVLGAVILGSTLLAG-S-1,2-diacyl-sn-glyceryl-L-cysteine -SSN
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.145 LGT
-
 Escherichia coli
2.5.1.145 phosphatidylglycerol:prolipoprotein diacylglyceryl transferase
-
 Escherichia coli
2.5.1.145 prolipoprotein diacylglyceryl transferase
-
 Escherichia coli