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Literature summary extracted from

  • Nakamura, T.; Iwahashi, H.; Eguchi, Y.
    Enzymatic proof for the identity of the S-sulfocysteine synthase and cysteine synthase B of Salmonella typhimurium (1984), J. Bacteriol., 158, 1122-1127 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.144 D-cycloserine 18% residual activity at 5 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.144 hydroxylamin 3% residual activity at 10 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.144 additional information not inhibited by L-methionine Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.144 S-sulfocysteine 74% residual activity at 5 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.144 Semicarbazide 40% residual activity at 1 mM Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.144 2.7
-
 thiosulfate at pH 7.6, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.144 9
-
 O-acetyl-L-serine at pH 7.6, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.144 O-acetyl-L-serine + thiosulfate Salmonella enterica subsp. enterica serovar Typhimurium
-
 S-sulfo-L-cysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + thiosulfate Salmonella enterica subsp. enterica serovar Typhimurium LT-2
-
 S-sulfo-L-cysteine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.144 Salmonella enterica subsp. enterica serovar Typhimurium P29848
-
-
2.5.1.144 Salmonella enterica subsp. enterica serovar Typhimurium LT-2 P29848
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.144 ammonium sulfate precipitation, DEAE-cellulose DE-32 column chromatography, DEAE-Sepharose CL-6B column chromatography, DEAE-cellulose DE-53 column chromatography, hydroxylapatite column chromatography, and Sephacryl S-200 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.144 0.13
-
 crude extract, at pH 7.6, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.144 276.6
-
 after 2212fold purification, at pH 7.6, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.144 azaserine + Na2S
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.5.1.144 azaserine + Na2S
-
 Salmonella enterica subsp. enterica serovar Typhimurium LT-2 ?
-
 ?
2.5.1.144 azaserine + thiosulfate
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.5.1.144 azaserine + thiosulfate
-
 Salmonella enterica subsp. enterica serovar Typhimurium LT-2 ?
-
 ?
2.5.1.144 additional information no activity with O-phosphoserine, O-succinylhomoserine, and L-serine Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.5.1.144 additional information no activity with O-phosphoserine, O-succinylhomoserine, and L-serine Salmonella enterica subsp. enterica serovar Typhimurium LT-2 ?
-
 ?
2.5.1.144 O-acetyl-L-serine + Na2S
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.5.1.144 O-acetyl-L-serine + Na2S
-
 Salmonella enterica subsp. enterica serovar Typhimurium LT-2 ?
-
 ?
2.5.1.144 O-acetyl-L-serine + thiosulfate
-
 Salmonella enterica subsp. enterica serovar Typhimurium S-sulfo-L-cysteine + acetate
-
 ?
2.5.1.144 O-acetyl-L-serine + thiosulfate
-
 Salmonella enterica subsp. enterica serovar Typhimurium LT-2 S-sulfo-L-cysteine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.144 ? x * 55000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
2.5.1.144 cysteine synthase B
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.144 65 77 the enzyme is inactivated by the treatment around 65 to 67.5°C, and practically complete inactivation is observed at 77.5°C Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.144 7.2
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.144 pyridoxal 5'-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium