EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.1.3.44 | sitting drop vapor diffusion method, using 100 mM KH2PO4/Na2HPO4 pH 6.2, 10% (w/v) PEG 3000 | Methanocaldococcus jannaschii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.3.44 | C195S | the mutant shows reduced activity compared tot he wild type enzyme | Methanocaldococcus jannaschii |
4.1.3.44 | K41A | inactive | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.44 | N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine | Methanocaldococcus jannaschii | - |
4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O | - |
ir | |
4.1.3.44 | N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine | Methanocaldococcus jannaschii DSM 2661 | - |
4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.3.44 | Methanocaldococcus jannaschii | Q57705 | - |
- |
4.1.3.44 | Methanocaldococcus jannaschii DSM 2661 | Q57705 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.3.44 | ammonium sulfate precipitation, butyl Sepharose column chromatography, and HiTrap chelating column chromatography | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.44 | N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine | - |
Methanocaldococcus jannaschii | 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O | - |
ir | |
4.1.3.44 | N1-methylguanine37 in tRNAPhe + pyruvate + S-adenosyl-L-methionine | - |
Methanocaldococcus jannaschii DSM 2661 | 4-demethylwyosine37 in tRNAPhe + L-methionine + 5'-deoxyadenosine + CO2 + H2O | - |
ir |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.3.44 | TYW1 | - |
Methanocaldococcus jannaschii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.3.44 | S-adenosyl-L-methionine | - |
Methanocaldococcus jannaschii | |
4.1.3.44 | [4Fe-4S]-center | - |
Methanocaldococcus jannaschii |