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Literature summary extracted from

  • Faridi, S.; Satyanarayana, T.
    Characteristics of recombinant alpha-carbonic anhydrase of polyextremophilic bacterium Bacillus halodurans TSLV1 (2016), Int. J. Biol. Macromol., 89, 659-668 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.1.1 additional information carbonic anhydrase is a biocatalyst that catalyzes the hydration of CO2 to bicarbonate and protons, thus useful in mitigating green house effect by sequestering CO2 from various point sources Halalkalibacterium halodurans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) achieving a 31.4fold enhancement in CA production for the wild-type, subcloning in Escherichia coli strain DH5alpha. About 50% of wild-type enzyme produced is secreted when recombinant Escherichia coli harboring BhCA-pET22b is cultivated in a medium with EDTA and lysozyme because of the efficient pelB leader sequence, cultvation parameters are optimized for rBhCA secretion Halalkalibacterium halodurans

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 2,6-PDCA
-
 Halalkalibacterium halodurans
4.2.1.1 2-mercaptoethanol 15% inhibition at 5 mM Halalkalibacterium halodurans
4.2.1.1 acetazolamide strong inhibition at 5 mM Halalkalibacterium halodurans
4.2.1.1 Ag+ inhibits by 17% at 5 mM, no inhibition at 1 mM Halalkalibacterium halodurans
4.2.1.1 Al3+ inhibits by 12% at 5 mM, no inhibition at 1 mM Halalkalibacterium halodurans
4.2.1.1 Co2+ inhibits by 74% at 5 mM Halalkalibacterium halodurans
4.2.1.1 Cu2+ inhibits completely at 5 mM, 66% inhibition at 1 mM Halalkalibacterium halodurans
4.2.1.1 diethyl dicarbonate strong inhibition at 5 mM Halalkalibacterium halodurans
4.2.1.1 DTT 60% inhibition at 5 mM Halalkalibacterium halodurans
4.2.1.1 EDTA
-
 Halalkalibacterium halodurans
4.2.1.1 ethyl 3-(3-dimethylaminopropyl)-carbodiimide 80% inhibition at 5 mM, via irreversible modification of carboxyl groups or thepresence of catalytically active tyrosine in the enzyme molecule Halalkalibacterium halodurans
4.2.1.1 Fe2+ inhibits completely at 5 mM, 74% inhibition at 1 mM Halalkalibacterium halodurans
4.2.1.1 Fe3+ inhibits completely at 5 mM, 75% inhibition at 1 mM Halalkalibacterium halodurans
4.2.1.1 Hg2+ inhibits completely at 5 mM, 78% inhibition at 1 mM Halalkalibacterium halodurans
4.2.1.1 iodoacetic acid
-
 Halalkalibacterium halodurans
4.2.1.1 N-bromosuccinimide strong inhibition at 5 mM Halalkalibacterium halodurans
4.2.1.1 N-ethylmaleimide strong inhibition at 5 mM Halalkalibacterium halodurans
4.2.1.1 Na+ inhibits by 25% at 2 mM Halalkalibacterium halodurans
4.2.1.1 Ni2+ inhibits by 84% at 5 mM, 80% inhibition at 1 mM Halalkalibacterium halodurans
4.2.1.1 Pb2+ inhibits by 30% at 5 mM Halalkalibacterium halodurans
4.2.1.1 PMSF 80% inhibition at 5 mM Halalkalibacterium halodurans
4.2.1.1 SDS
-
 Halalkalibacterium halodurans
4.2.1.1 Triton X-100
-
 Halalkalibacterium halodurans
4.2.1.1 Tween 80
-
 Halalkalibacterium halodurans
4.2.1.1 Woodward's reagent K WRK Halalkalibacterium halodurans
4.2.1.1 Zn2+ inhibits by 95% at 5 mM Halalkalibacterium halodurans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Mn2+ BhCA also functions as an efficient virtual peroxidase when Zn2+ is substituted with Mn2+ Halalkalibacterium halodurans
4.2.1.1 additional information no effect on enzyme activity at 5 mM by Ca2+, NH4+, Mg2+, Ba2+, and Mn2+ Halalkalibacterium halodurans
4.2.1.1 Sn2+ activates about 40% at 5 mM Halalkalibacterium halodurans
4.2.1.1 Zn2+ required, metalloenzyme Halalkalibacterium halodurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.1 75000
-
 about Halalkalibacterium halodurans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 CO2 + H2O Halalkalibacterium halodurans
-
 H2CO3
-
 r
4.2.1.1 CO2 + H2O Halalkalibacterium halodurans TSLV1
-
 H2CO3
-
 r
4.2.1.1 H2CO3 Halalkalibacterium halodurans
-
 CO2 + H2O
-
 r
4.2.1.1 H2CO3 Halalkalibacterium halodurans TSLV1
-
 CO2 + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Halalkalibacterium halodurans A0A0N9WRG3
-
-
4.2.1.1 Halalkalibacterium halodurans TSLV1 A0A0N9WRG3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) medium by nickel affinity chromatography Halalkalibacterium halodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 CO2 + H2O
-
 Halalkalibacterium halodurans H2CO3
-
 r
4.2.1.1 CO2 + H2O
-
 Halalkalibacterium halodurans TSLV1 H2CO3
-
 r
4.2.1.1 H2CO3
-
 Halalkalibacterium halodurans CO2 + H2O
-
 r
4.2.1.1 H2CO3
-
 Halalkalibacterium halodurans TSLV1 CO2 + H2O
-
 r
4.2.1.1 additional information mineralization of CO2 using recombinant BhCA leads to accelerated precipitation of CaCO3 in calcite form. Recombinant BhCA also functions as an efficient virtual peroxidase when Zn2+ is substituted with Mn2+ Halalkalibacterium halodurans ?
-
 ?
4.2.1.1 additional information mineralization of CO2 using recombinant BhCA leads to accelerated precipitation of CaCO3 in calcite form. Recombinant BhCA also functions as an efficient virtual peroxidase when Zn2+ is substituted with Mn2+ Halalkalibacterium halodurans TSLV1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.1 homodimer
-
 Halalkalibacterium halodurans
4.2.1.1 More structural secondary composition of rBhCA at different pH values, overview Halalkalibacterium halodurans

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 alpha carbonic anhydrase
-
 Halalkalibacterium halodurans
4.2.1.1 alpha-carbonic anhydrase
-
 Halalkalibacterium halodurans
4.2.1.1 BhCA
-
 Halalkalibacterium halodurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.1 37
-
 assay at Halalkalibacterium halodurans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.1 50 72 Tm of BhCA is 72°C, T1/2 values at 50°C and 60°C are 66 min and 24 min, respectively Halalkalibacterium halodurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.1 8.3
-
 assay at Halalkalibacterium halodurans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.1 6 11 purified recombinant enzyme, completely stable within this range for 48 h Halalkalibacterium halodurans

General Information

EC Number General Information Comment Organism
4.2.1.1 additional information residues H137, H139, H156, and H110 present in the active site play an important role in catalysis, the active site of recombinant BhCA is also composed of conserved Glu and Asp acid residues Halalkalibacterium halodurans
4.2.1.1 physiological function carbonic anhydrase is a biocatalyst that catalyzes the hydration of CO2to bicarbonate and protons, and an alkalistable and moderately thermostable alpha-carbonic anhydrase Halalkalibacterium halodurans