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Literature summary extracted from

  • Soni, A.; Goyal, M.; Prakash, K.; Bhardwaj, J.; Siddiqui, A.J.; Puri, S.K.
    Cloning, expression and functional characterization of heme detoxification protein (HDP) from the rodent malaria parasite Plasmodium vinckei (2015), Gene, 566, 109-119 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.99.1.8 expression in Escherichia coli Plasmodium vinckei petteri

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.99.1.8 Chloroquine
-
 Plasmodium vinckei petteri
4.99.1.8 diethyldicarbonate histidine modification using diethyldicarbonate leads to reduction in heme binding and hemozoin formation Plasmodium vinckei petteri
4.99.1.8 mefloquine
-
 Plasmodium vinckei petteri
4.99.1.8 additional information not inhibitory up to 0.04 mM: pyrimethamine Plasmodium vinckei petteri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.99.1.8 cytosol
-
 Plasmodium vinckei petteri 5829
-

Organism

EC Number Organism UniProt Comment Textmining
4.99.1.8 Plasmodium vinckei petteri F5BWX4
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.99.1.8 additional information enzyme is expressed throughout the intra erythrocytic stages Plasmodium vinckei petteri
-
4.99.1.8 schizont expression is increased in late trophozoite and schizont stages of parasite Plasmodium vinckei petteri
-
4.99.1.8 trophozoite expression is increased in late trophozoite and schizont stages of parasite Plasmodium vinckei petteri
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.99.1.8 heme enzyme binds heme with a very high affinity, KD value 89 nM Plasmodium vinckei petteri hemozoin
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.99.1.8 90
-
 10 min, 70% residual activity Plasmodium vinckei petteri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.99.1.8 5.2
-
 assay at Plasmodium vinckei petteri

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.99.1.8 3 5.5
-
 Plasmodium vinckei petteri

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.99.1.8 0.01
-
 pH 5.2, 37°C Plasmodium vinckei petteri Chloroquine
4.99.1.8 0.025
-
 pH 5.2, 37°C Plasmodium vinckei petteri mefloquine