Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hamal, A.; Forterre, P.; Elie, C.
    Purification and characterization of a DNA polymerase from the archaebacterium Thermoplasma acidophilum (1990), Eur. J. Biochem., 190, 517-521 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.7 ethyleneglycol 5%, activity is stimulated by 30% Thermoplasma acidophilum
2.7.7.7 glycerol 5%, activity is stimulated by 30% Thermoplasma acidophilum
2.7.7.7 additional information no stimulation with poly(ethy1ene glycol) Thermoplasma acidophilum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 2',3'-dideoxyribosylthymine triphosphate the enzyme is slightly sensitive to. 25% inhibition is observed with a ddTTP/dTTP ratio of 50 Thermoplasma acidophilum
2.7.7.7 additional information the enzyme is resistant to aphidicolin Thermoplasma acidophilum
2.7.7.7 N-ethylmaleimide inhibited when preincubation with this reagent is performed at 65°C Thermoplasma acidophilum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 MgCl2 optimal activity is obtained in the presence of 4 mM MgCl2 Thermoplasma acidophilum
2.7.7.7 MnCl2 sustitution of MgCl2 by MnCl2 produces a slight decrease of activity. Optimal activity is obtained in the presence of 0.4 mM MgCl2 Thermoplasma acidophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 88000
-
 gel filtration Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Thermoplasma acidophilum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7
-
 Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 additional information a 3'->5' exonuclease activity is associated with the purified DNA polymerase Thermoplasma acidophilum ?
-
 ?
2.7.7.7 poly(rA)/(dT)12 + dTTP
-
 Thermoplasma acidophilum poly(rA)/(dT)13 + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.7 monomer 1 * 88000, SDS-PAGE Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.7 65
-
 DNA polymerase activity and 3'->5' exonuclease activity Thermoplasma acidophilum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.7 50
-
 55% of maximal activity, DNA polymerase activity Thermoplasma acidophilum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.7 65
-
 stable up to Thermoplasma acidophilum
2.7.7.7 75
-
 30 min, 50% loss of activity Thermoplasma acidophilum
2.7.7.7 85
-
 30 min, vomplete inactivation Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 8
-
-
 Thermoplasma acidophilum