BRENDA - Enzyme Database

Production, purification and partial characterization for an oxalate decarboxylase (OxDcase) from the probiote Lactobacillus plantarum KSK-II

Kotb, E.; Curr. Sci. 114, 835-844 (2018)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.2
arabic gum
about 120% activity at 0.1% (w/v)
Lactobacillus plantarum
4.1.1.2
arabinogalactan
about 150% activity at 0.1% (w/v)
Lactobacillus plantarum
4.1.1.2
ferrocyanide
about 112% activity at 5 mM
Lactobacillus plantarum
4.1.1.2
fructooligosaccharide
about 160% activity at 0.1% (w/v)
Lactobacillus plantarum
4.1.1.2
guar gum
about 140% activity at 0.1% (w/v)
Lactobacillus plantarum
4.1.1.2
inulin
about 110% activity at 0.1% (w/v)
Lactobacillus plantarum
4.1.1.2
L-ascorbate
about 110% activity at 5 mM
Lactobacillus plantarum
4.1.1.2
lactitol
about 130% activity at 0.1% (w/v)
Lactobacillus plantarum
4.1.1.2
maltodextrin
about 145% activity at 0.1% (w/v)
Lactobacillus plantarum
4.1.1.2
o-phenylenediamine
about 138% activity at 5 mM
Lactobacillus plantarum
4.1.1.2
Triton X-100
about 115% activity at 5 mM
Lactobacillus plantarum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.2
2-oxoglutaric acid
about 82% residual activity at 25 mM
Lactobacillus plantarum
4.1.1.2
Citric acid
about 77% residual activity at 25 mM
Lactobacillus plantarum
4.1.1.2
Fumaric acid
about 73% residual activity at 25 mM
Lactobacillus plantarum
4.1.1.2
hydroxylamine
about 50% residual activity at 5 mM
Lactobacillus plantarum
4.1.1.2
Maleic acid
about 69% residual activity at 25 mM
Lactobacillus plantarum
4.1.1.2
malonic acid
about 68% residual activity at 25 mM
Lactobacillus plantarum
4.1.1.2
NaBr
about 71% residual activity at 50 mM
Lactobacillus plantarum
4.1.1.2
NaCl
about 98% residual activity at 50 mM
Lactobacillus plantarum
4.1.1.2
NaF
about 68% residual activity at 50 mM
Lactobacillus plantarum
4.1.1.2
NaI
about 82% residual activity at 50 mM
Lactobacillus plantarum
4.1.1.2
oxaloacetic acid
about 68% residual activity at 25 mM
Lactobacillus plantarum
4.1.1.2
Phthalic acid
about 97% residual activity at 25 mM
Lactobacillus plantarum
4.1.1.2
Sodium dithionite
about 75% residual activity at 5 mM
Lactobacillus plantarum
4.1.1.2
sodium sulfite
about 60% residual activity at 5 mM
Lactobacillus plantarum
4.1.1.2
succinic acid
about 77% residual activity at 25 mM
Lactobacillus plantarum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.2
12.7
-
oxalate
at pH 5.0 and 35C
Lactobacillus plantarum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.1.2
oxalate + H+
Lactobacillus plantarum
-
formate + CO2
-
-
?
4.1.1.2
oxalate + H+
Lactobacillus plantarum KSK-II
-
formate + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.1.2
Lactobacillus plantarum
-
-
-
4.1.1.2
Lactobacillus plantarum KSK-II
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.1.2
DEAE-Sepharose column chromatography and Sephadex G-100 gel filtration
Lactobacillus plantarum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.2
oxalate + H+
-
747628
Lactobacillus plantarum
formate + CO2
-
-
-
?
4.1.1.2
oxalate + H+
-
747628
Lactobacillus plantarum KSK-II
formate + CO2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.1.2
?
x * 63000, SDS-PAGE
Lactobacillus plantarum
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.1.1.2
35
-
-
Lactobacillus plantarum
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
4.1.1.2
20
50
more than 70% activity between 20 and 50C
Lactobacillus plantarum
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.1.1.2
47
-
the enzyme is stable at pH 4.0-7.0 for 1 h
Lactobacillus plantarum
4.1.1.2
70
-
the enzyme is maximally stable till 70?C for 1 h
Lactobacillus plantarum
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.1.2
64
-
oxalate
at pH 5.0 and 35C
Lactobacillus plantarum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.2
5
-
-
Lactobacillus plantarum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.2
4
6
more than 70% activity between pH 4.0 and 6.0
Lactobacillus plantarum
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.1.1.2
Lactobacillus plantarum
isoelectric focusing
-
3.5
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.1.2
arabic gum
about 120% activity at 0.1% (w/v)
Lactobacillus plantarum
4.1.1.2
arabinogalactan
about 150% activity at 0.1% (w/v)
Lactobacillus plantarum
4.1.1.2
ferrocyanide
about 112% activity at 5 mM
Lactobacillus plantarum
4.1.1.2
fructooligosaccharide
about 160% activity at 0.1% (w/v)
Lactobacillus plantarum
4.1.1.2
guar gum
about 140% activity at 0.1% (w/v)
Lactobacillus plantarum
4.1.1.2
inulin
about 110% activity at 0.1% (w/v)
Lactobacillus plantarum
4.1.1.2
L-ascorbate
about 110% activity at 5 mM
Lactobacillus plantarum
4.1.1.2
lactitol
about 130% activity at 0.1% (w/v)
Lactobacillus plantarum
4.1.1.2
maltodextrin
about 145% activity at 0.1% (w/v)
Lactobacillus plantarum
4.1.1.2
o-phenylenediamine
about 138% activity at 5 mM
Lactobacillus plantarum
4.1.1.2
Triton X-100
about 115% activity at 5 mM
Lactobacillus plantarum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.1.2
2-oxoglutaric acid
about 82% residual activity at 25 mM
Lactobacillus plantarum
4.1.1.2
Citric acid
about 77% residual activity at 25 mM
Lactobacillus plantarum
4.1.1.2
Fumaric acid
about 73% residual activity at 25 mM
Lactobacillus plantarum
4.1.1.2
hydroxylamine
about 50% residual activity at 5 mM
Lactobacillus plantarum
4.1.1.2
Maleic acid
about 69% residual activity at 25 mM
Lactobacillus plantarum
4.1.1.2
malonic acid
about 68% residual activity at 25 mM
Lactobacillus plantarum
4.1.1.2
NaBr
about 71% residual activity at 50 mM
Lactobacillus plantarum
4.1.1.2
NaCl
about 98% residual activity at 50 mM
Lactobacillus plantarum
4.1.1.2
NaF
about 68% residual activity at 50 mM
Lactobacillus plantarum
4.1.1.2
NaI
about 82% residual activity at 50 mM
Lactobacillus plantarum
4.1.1.2
oxaloacetic acid
about 68% residual activity at 25 mM
Lactobacillus plantarum
4.1.1.2
Phthalic acid
about 97% residual activity at 25 mM
Lactobacillus plantarum
4.1.1.2
Sodium dithionite
about 75% residual activity at 5 mM
Lactobacillus plantarum
4.1.1.2
sodium sulfite
about 60% residual activity at 5 mM
Lactobacillus plantarum
4.1.1.2
succinic acid
about 77% residual activity at 25 mM
Lactobacillus plantarum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.1.2
12.7
-
oxalate
at pH 5.0 and 35C
Lactobacillus plantarum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.1.2
oxalate + H+
Lactobacillus plantarum
-
formate + CO2
-
-
?
4.1.1.2
oxalate + H+
Lactobacillus plantarum KSK-II
-
formate + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.1.2
DEAE-Sepharose column chromatography and Sephadex G-100 gel filtration
Lactobacillus plantarum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.1.2
oxalate + H+
-
747628
Lactobacillus plantarum
formate + CO2
-
-
-
?
4.1.1.2
oxalate + H+
-
747628
Lactobacillus plantarum KSK-II
formate + CO2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.1.2
?
x * 63000, SDS-PAGE
Lactobacillus plantarum
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.1.1.2
35
-
-
Lactobacillus plantarum
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
4.1.1.2
20
50
more than 70% activity between 20 and 50C
Lactobacillus plantarum
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.1.1.2
47
-
the enzyme is stable at pH 4.0-7.0 for 1 h
Lactobacillus plantarum
4.1.1.2
70
-
the enzyme is maximally stable till 70?C for 1 h
Lactobacillus plantarum
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
4.1.1.2
64
-
oxalate
at pH 5.0 and 35C
Lactobacillus plantarum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.1.2
5
-
-
Lactobacillus plantarum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.1.1.2
4
6
more than 70% activity between pH 4.0 and 6.0
Lactobacillus plantarum
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.1.1.2
Lactobacillus plantarum
isoelectric focusing
-
3.5
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.1.1.2
5.05
-
oxalate
at pH 5.0 and 35C
Lactobacillus plantarum
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.1.1.2
5.05
-
oxalate
at pH 5.0 and 35C
Lactobacillus plantarum