BRENDA - Enzyme Database show

Crystallizing membrane proteins in the lipidic mesophase. Experience with human prostaglandin E2 synthase 1 and an evolving strategy

Li, D.; Howe, N.; Dukkipati, A.; Shah, S.T.; Bax, B.D.; Edge, C.; Bridges, A.; Hardwicke, P.; Singh, O.M.; Giblin, G.; Pautsch, A.; Pfau, R.; Schnapp, G.; Wang, M.; Olieric, V.; Caffrey, M.; Cryst. Growth Des. 14, 2034-2047 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.3
expressed in Spodoptera frugiperda Sf 9 cells
Homo sapiens
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.3.99.3
-
Homo sapiens
General Stability
EC Number
General Stability
Organism
5.3.99.3
reduced glutathione is essential to stabilize the enzyme
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Homo sapiens
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.3
126000
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.3
Prostaglandin H2
Homo sapiens
-
Prostaglandin E2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.3
Homo sapiens
O14684
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.3
Ni-NTA resin column chromatography and Superdex 200 gel filtration
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin H2
-
747613
Homo sapiens
Prostaglandin E2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
5.3.99.3
heptamer
7 * 18000, SDS-PAGE
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
expressed in Spodoptera frugiperda Sf 9 cells
Homo sapiens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.3.99.3
-
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
5.3.99.3
reduced glutathione is essential to stabilize the enzyme
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Homo sapiens
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.3
126000
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.3
Prostaglandin H2
Homo sapiens
-
Prostaglandin E2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
Ni-NTA resin column chromatography and Superdex 200 gel filtration
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin H2
-
747613
Homo sapiens
Prostaglandin E2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.99.3
heptamer
7 * 18000, SDS-PAGE
Homo sapiens