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Literature summary extracted from

  • Esbaugh, A.J.; Secor, S.M.; Grosell, M.
    Characterization of carbonic anhydrase XIII in the erythrocytes of the Burmese python, Python molurus bivittatus (2015), Comp. Biochem. Physiol. B, 187, 71-77 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 cloning of isozyme CA XIII, phylogenetic analysis, quantitative real-time PCR expression analysis Python bivittatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 azetazolamide
-
 Python bivittatus
4.2.1.1 CNO-
-
 Python bivittatus
4.2.1.1 Cu2+
-
 Python bivittatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 21.5
-
 H2CO3 pH not specified in the publication, 4°C, assay using the delta pH method Python bivittatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.1 cytoplasm
-
 Python bivittatus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Zn2+ required, metalloenzyme Python bivittatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 H2CO3 Python bivittatus
-
 CO2 + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Python bivittatus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.1 erythrocyte about 90% of the CA activity in erythrocytes is due to the high activity CA II-like isoform, 10% are due to a low activity CA XIII isozyme Python bivittatus
-
4.2.1.1 erythrocyte erythrocyte isoform CA XIII. About 90% of the CA activity in erythrocytes is due to a high activity CA II-like isoform, 10% are due to the low activity CA XIII isozyme Python bivittatus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 H2CO3
-
 Python bivittatus CO2 + H2O
-
 r
4.2.1.1 additional information assay using the delta pH method at 4°C Python bivittatus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 alpha-carbonic anhydrase
-
 Python bivittatus
4.2.1.1 carbonic anhydrase II
-
 Python bivittatus
4.2.1.1 carbonic anhydrase XIII
-
 Python bivittatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.1 13000
-
 H2CO3 pH not specified in the publication, 4°C, assay using the delta pH method Python bivittatus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.1 0.00000064
-
 azetazolamide pH not specified in the publication, 4°C, assay using the delta pH method Python bivittatus
4.2.1.1 0.0004
-
 Cu2+ pH not specified in the publication, 4°C, assay using the delta pH method Python bivittatus
4.2.1.1 0.0024
-
 CNO- pH not specified in the publication, 4°C, assay using the delta pH method Python bivittatus
4.2.1.1 0.0309
-
 Cu2+ pH not specified in the publication, 4°C, assay using the delta pH method Python bivittatus

Expression

EC Number Organism Comment Expression
4.2.1.1 Python bivittatus feeding stress is used to upregulate CO2 excretion pathways: significant upregulation of isozyme CA II is observed, while isozyme CA XIII is strongly downregulated down

General Information

EC Number General Information Comment Organism
4.2.1.1 physiological function carbonic anhydrase (CA) is a ubiquitously expressed metalloenzyme that catalyses the hydration and dehydration reactions of carbon dioxide and bicarbonate, respectively. This basic reaction has innumerable physiological and biochemical associations and consequently CA isoforms are found in virtually all living organisms Python bivittatus