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Literature summary extracted from

  • Hansen, K.; Varvas, K.; Jaerving, I.; Samel, N.
    Novel membrane-associated prostaglandin E synthase-2 from crustacean arthropods (2014), Comp. Biochem. Physiol. B, 174, 45-52 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.3.99.3 GSH the enzyme activity is increased in the presence of reduced glutathione Gammarus sp. KV-2010a
5.3.99.3 GSH the enzyme activity is increased in the presence of reduced glutathione Caprella sp. KV-2010a

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.3 expressed in Escherichia coli BL21(DE3)RP cells Gammarus sp. KV-2010a
5.3.99.3 expressed in Escherichia coli BL21(DE3)RP cells Caprella sp. KV-2010a

General Stability

EC Number General Stability Organism
5.3.99.3 the enzyme stability is increased in the presence of reduced glutathione Gammarus sp. KV-2010a
5.3.99.3 the enzyme stability is increased in the presence of reduced glutathione Caprella sp. KV-2010a

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.99.3 4-hydroxymercuribenzoate
-
Caprella sp. KV-2010a
5.3.99.3 4-hydroxymercuribenzoate
-
Gammarus sp. KV-2010a

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.99.3 membrane
-
Gammarus sp. KV-2010a 16020
-
5.3.99.3 membrane
-
Caprella sp. KV-2010a 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.99.3 Prostaglandin H2 Gammarus sp. KV-2010a
-
Prostaglandin E2
-
?
5.3.99.3 Prostaglandin H2 Caprella sp. KV-2010a
-
Prostaglandin E2
-
?

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.3 Caprella sp. KV-2010a S4VIU3
-
-
5.3.99.3 Gammarus sp. KV-2010a S4VLK7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.99.3 Ni-NTA column chromatography, and Superdex 200 gel filtration Gammarus sp. KV-2010a
5.3.99.3 Ni-NTA column chromatography, and Superdex 200 gel filtration Caprella sp. KV-2010a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.3 Prostaglandin H2
-
Gammarus sp. KV-2010a Prostaglandin E2
-
?
5.3.99.3 Prostaglandin H2
-
Caprella sp. KV-2010a Prostaglandin E2
-
?

Subunits

EC Number Subunits Comment Organism
5.3.99.3 ? x * 39000, N-terminally truncated His6-tagged enzyme, SDS-PAGE Gammarus sp. KV-2010a
5.3.99.3 ? x * 39000, N-terminally truncated His6-tagged enzyme, SDS-PAGE Caprella sp. KV-2010a

Synonyms

EC Number Synonyms Comment Organism
5.3.99.3 PGE synthase
-
Gammarus sp. KV-2010a
5.3.99.3 PGE synthase
-
Caprella sp. KV-2010a
5.3.99.3 PGES-2
-
Gammarus sp. KV-2010a
5.3.99.3 PGES-2
-
Caprella sp. KV-2010a
5.3.99.3 prostaglandin E synthase-2
-
Gammarus sp. KV-2010a
5.3.99.3 prostaglandin E synthase-2
-
Caprella sp. KV-2010a