Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Abidin, M.; Saravanan, T.; Zhang, J.; Tepper, P.; Strauss, E.; Poelarends, G.
    Modular enzymatic cascade synthesis of vitamin B5 and its derivatives (2018), Chemistry, 24, 17434-17438 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.3.1.2 synthesis starting from simple non-chiral dicarboxylic acids (either fumaric acid or mesaconic acid), vitamin B5 and both diastereoisomers of alpha-methyl-substituted vitamin B5, which are valuable precursors for promising antimicrobials against Plasmodium falciparum and multidrug-resistant Staphylococcus aureus, can be generated in good yields (up to 70%) and excellent enantiopurity (>99% ee). Access to vitamin B5 ((R)-pantothenic acid) and both diastereoisomers of alpha-methyl-substituted vitamin B5 ((R)- and (S)-3-((R)-2,4-dihydroxy-3,3-dimethylbutanamido)-2-methylpropanoic acid) is achieved using a modular three-step biocatalytic cascade involving 3-methylaspartate ammonia lyase (MAL), aspartate-a-decarboxylase (ADC), beta-methylaspartate-alpha-decarboxylase (CrpG) or glutamate decarboxylase (GAD), and pantothenate synthetase (PS) enzymes Clostridium tetanomorphum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.1.2 L-threo-3-methylaspartate Clostridium tetanomorphum the enzyme is part of a catabolic pathway for L-glutamate mesaconate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.2 Clostridium tetanomorphum Q05514
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.2 L-threo-3-methylaspartate
-
 Clostridium tetanomorphum mesaconate + NH3
-
 r
4.3.1.2 L-threo-3-methylaspartate the enzyme is part of a catabolic pathway for L-glutamate Clostridium tetanomorphum mesaconate + NH3
-
 ?
4.3.1.2 mesaconate + NH3
-
 Clostridium tetanomorphum L-threo-3-methylaspartate
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.3.1.2 3-methylaspartate ammonia lyase
-
 Clostridium tetanomorphum
4.3.1.2 MAL
-
 Clostridium tetanomorphum

General Information

EC Number General Information Comment Organism
4.3.1.2 metabolism the enzyme is part of a catabolic pathway for L-glutamate Clostridium tetanomorphum