Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kudo, F.; Kawamura, K.; Uchino, A.; Miyanaga, A.; Numakura, M.; Takayanagi, R.; Eguchi, T.
    Genome mining of the hitachimycin biosynthetic gene cluster involvement of a phenylalanine-2,3-aminomutase in biosynthesis (2015), ChemBioChem, 16, 909-914 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.3.11 gene hitA, cDNA library construction, genome mining of hitachimycin biosynthetic gene cluster, DNA and amino acid sequence determination and analysis. The identified gene cluster is responsible for the biosynthesis of hitachimycin. Recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Embleya scabrispora

Protein Variants

EC Number Protein Variants Comment Organism
5.4.3.11 additional information construction of a hitA knockout mutant strain Embleya scabrispora

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.3.11 L-phenylalanine Embleya scabrispora
-
 D-beta-phenylalanine
-
 ?
5.4.3.11 L-phenylalanine Embleya scabrispora JCM 11712 / DSM 41855
-
 D-beta-phenylalanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.3.11 Embleya scabrispora A0A0F7R1D9
-
-
5.4.3.11 Embleya scabrispora JCM 11712 / DSM 41855 A0A0F7R1D9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.3.11 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, desalting gel filtration, and ultrafiltration Embleya scabrispora

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.3.11 L-phenylalanine
-
 Embleya scabrispora D-beta-phenylalanine
-
 ?
5.4.3.11 L-phenylalanine
-
 Embleya scabrispora JCM 11712 / DSM 41855 D-beta-phenylalanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.3.11 HitA
-
 Embleya scabrispora
5.4.3.11 PAM
-
 Embleya scabrispora
5.4.3.11 phenylalanine-2,3-aminomutase
-
 Embleya scabrispora

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.3.11 28
-
 assay at Embleya scabrispora

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.3.11 8.5
-
 assay at Embleya scabrispora

General Information

EC Number General Information Comment Organism
5.4.3.11 malfunction a hitA knockout mutant shows no hitachimycin production, but antibiotic production is restored by feeding with (S)-beta-Phe Embleya scabrispora
5.4.3.11 metabolism the enzyme is important in the hitachimycin biosynthetic pathway. A plausible biosynthetic pathway for hitachimycin, including a unique polyketide skeletal transformation mechanism, is proposed Embleya scabrispora