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Literature summary extracted from

  • de Ruyck, J.; Janczak, M.W.; Neti, S.S.; Rothman, S.C.; Schubert, H.L.; Cornish, R.M.; Matagne, A.; Wouters, J.; Poulter, C.D.
    Determination of kinetics and the crystal structure of a novel type 2 isopentenyl diphosphate dimethylallyl diphosphate isomerase from Streptococcus pneumoniae (2014), ChemBioChem, 15, 1452-1458 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.2 expressed in Escherichia coli M15[pREP4] cells Streptococcus pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.3.2 sitting drop vapor diffusion method, using HEPES buffer (100 mM, pH 7.5 with 2 M (NH4)2SO4) Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.3.2 FMN
-
 Streptococcus pneumoniae
5.3.3.2 isopentenyl diphosphate
-
 Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.3.2 0.04
-
 isopentenyl diphosphate at pH 7.0 and 37°C Streptococcus pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.3.2 173600
-
 gel filtration Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.3.2 isopentenyl diphosphate Streptococcus pneumoniae
-
 dimethylallyl diphosphate
-
 r
5.3.3.2 isopentenyl diphosphate Streptococcus pneumoniae CGSP14
-
 dimethylallyl diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.2 Streptococcus pneumoniae B2ILS5
-
-
5.3.3.2 Streptococcus pneumoniae CGSP14 B2ILS5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.3.2 His-Trap column chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.2 isopentenyl diphosphate
-
 Streptococcus pneumoniae dimethylallyl diphosphate
-
 r
5.3.3.2 isopentenyl diphosphate
-
 Streptococcus pneumoniae CGSP14 dimethylallyl diphosphate
-
 r

Subunits

EC Number Subunits Comment Organism
5.3.3.2 homotetramer 4 * 39868, calculated from amino acid sequence Streptococcus pneumoniae
5.3.3.2 homotetramer 4 * 39874, purified His-tagged enzyme, electrospray ionization mass spectrometry Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
5.3.3.2 Idi-2
-
 Streptococcus pneumoniae
5.3.3.2 type 2 isopentenyl diphosphate: dimethylallyl diphosphate isomerase
-
 Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.3.2 0.141
-
 isopentenyl diphosphate at pH 7.0 and 37°C Streptococcus pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
5.3.3.2 FMNH2
-
 Streptococcus pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.3.3.2 0.0064
-
 FMN at pH 7.0 and 37°C Streptococcus pneumoniae
5.3.3.2 0.292
-
 isopentenyl diphosphate at pH 7.0 and 37°C Streptococcus pneumoniae