EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.3.2 | expressed in Escherichia coli M15[pREP4] cells | Streptococcus pneumoniae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.3.3.2 | sitting drop vapor diffusion method, using HEPES buffer (100 mM, pH 7.5 with 2 M (NH4)2SO4) | Streptococcus pneumoniae |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.3.3.2 | FMN | - |
Streptococcus pneumoniae | |
5.3.3.2 | isopentenyl diphosphate | - |
Streptococcus pneumoniae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.3.2 | 0.04 | - |
isopentenyl diphosphate | at pH 7.0 and 37°C | Streptococcus pneumoniae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.3.3.2 | 173600 | - |
gel filtration | Streptococcus pneumoniae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.3.2 | isopentenyl diphosphate | Streptococcus pneumoniae | - |
dimethylallyl diphosphate | - |
r | |
5.3.3.2 | isopentenyl diphosphate | Streptococcus pneumoniae CGSP14 | - |
dimethylallyl diphosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.3.2 | Streptococcus pneumoniae | B2ILS5 | - |
- |
5.3.3.2 | Streptococcus pneumoniae CGSP14 | B2ILS5 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.3.2 | His-Trap column chromatography | Streptococcus pneumoniae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.3.2 | isopentenyl diphosphate | - |
Streptococcus pneumoniae | dimethylallyl diphosphate | - |
r | |
5.3.3.2 | isopentenyl diphosphate | - |
Streptococcus pneumoniae CGSP14 | dimethylallyl diphosphate | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
5.3.3.2 | homotetramer | 4 * 39868, calculated from amino acid sequence | Streptococcus pneumoniae |
5.3.3.2 | homotetramer | 4 * 39874, purified His-tagged enzyme, electrospray ionization mass spectrometry | Streptococcus pneumoniae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.3.2 | Idi-2 | - |
Streptococcus pneumoniae |
5.3.3.2 | type 2 isopentenyl diphosphate: dimethylallyl diphosphate isomerase | - |
Streptococcus pneumoniae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.3.2 | 0.141 | - |
isopentenyl diphosphate | at pH 7.0 and 37°C | Streptococcus pneumoniae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.3.3.2 | FMNH2 | - |
Streptococcus pneumoniae |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.3.2 | 0.0064 | - |
FMN | at pH 7.0 and 37°C | Streptococcus pneumoniae | |
5.3.3.2 | 0.292 | - |
isopentenyl diphosphate | at pH 7.0 and 37°C | Streptococcus pneumoniae |