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Literature summary extracted from

  • Wang, S.; Laborda, P.; Lu, A.; Duan, X.; Ma, H.; Liu, L.; Voglmeir, J.
    N-acetylglucosamine 2-epimerase from Pedobacter heparinus First experimental evidence of a deprotonation/reprotonation mechanism (2016), Catalysts, 6, 212-228 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.1.3.8 ATP over 4fold activation at 2 mM Pedobacter heparinus
5.1.3.8 additional information no effects by nucleotides AMP, ADP, GMP, GDP, GTP, CMP, CTP, and UTP at 2 mM each Pedobacter heparinus

Application

EC Number Application Comment Organism
5.1.3.8 additional information the mammalian enzyme is a target for development inhibitors for sialic acid biosynthesis Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.3.8 2-mercaptoethanol 30% inhibition at 10 mM, 40% at 100 mM Pedobacter heparinus
5.1.3.8 SDS 40% inhibition at 1% Pedobacter heparinus
5.1.3.8 Triton X-100 30% inhibition at 0.1%, 20% at 1% Pedobacter heparinus
5.1.3.8 Urea 50% inhibition at 2 M, 10% at 1 M Pedobacter heparinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.3.8 N-acetyl-D-glucosamine Homo sapiens
-
 N-acetyl-D-mannosamine
-
 r
5.1.3.8 N-acetyl-D-glucosamine Pedobacter heparinus
-
 N-acetyl-D-mannosamine
-
 r
5.1.3.8 N-acetyl-D-glucosamine Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3
-
 N-acetyl-D-mannosamine
-
 r
5.1.3.8 N-Acyl-D-glucosamine Homo sapiens
-
 N-Acyl-D-mannosamine
-
 r
5.1.3.8 N-Acyl-D-glucosamine Pedobacter heparinus
-
 N-Acyl-D-mannosamine
-
 r
5.1.3.8 N-Acyl-D-glucosamine Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3
-
 N-Acyl-D-mannosamine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.8 Homo sapiens P51606
-
-
5.1.3.8 Pedobacter heparinus C6Y403
-
-
5.1.3.8 Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3 C6Y403
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
5.1.3.8 N-acyl-D-glucosamine = N-acyl-D-mannosamine various amino acid residues of the enzyme are involved in the reaction mechanism Homo sapiens
a
5.1.3.8 N-acyl-D-glucosamine = N-acyl-D-mannosamine various amino acid residues of the enzyme are involved in the reaction mechanism Pedobacter heparinus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.1.3.8 kidney
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.3.8 N-acetyl-D-glucosamine
-
 Homo sapiens N-acetyl-D-mannosamine
-
 r
5.1.3.8 N-acetyl-D-glucosamine
-
 Pedobacter heparinus N-acetyl-D-mannosamine
-
 r
5.1.3.8 N-acetyl-D-glucosamine
-
 Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3 N-acetyl-D-mannosamine
-
 r
5.1.3.8 N-Acyl-D-glucosamine
-
 Homo sapiens N-Acyl-D-mannosamine
-
 r
5.1.3.8 N-Acyl-D-glucosamine
-
 Pedobacter heparinus N-Acyl-D-mannosamine
-
 r
5.1.3.8 N-Acyl-D-glucosamine
-
 Pedobacter heparinus ATCC 13125 / DSM 2366 / CIP 104194 / JCM 7457 / NBRC 12017 / NCIMB 9290 / NRRL B-14731 / HIM 762-3 N-Acyl-D-mannosamine
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.1.3.8 GlcNAc 2-epimerase
-
 Homo sapiens
5.1.3.8 GlcNAc 2-epimerase
-
 Pedobacter heparinus
5.1.3.8 N-Acetylglucosamine 2-epimerase
-
 Homo sapiens
5.1.3.8 N-Acetylglucosamine 2-epimerase
-
 Pedobacter heparinus
5.1.3.8 PhGn2E
-
 Pedobacter heparinus

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.3.8 NTP
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
5.1.3.8 evolution mammalian enzyme requires the presence of nucleotide activators and cofactors, while the bacterial enzyme are activated by nucleotide binding, but do not require them as cofactors Homo sapiens
5.1.3.8 evolution mammalian enzyme requires the presence of nucleotide activators and cofactors, while the bacterial enzyme are activated by nucleotide binding, but do not require them as cofactors Pedobacter heparinus
5.1.3.8 metabolism the biosynthesis of N-acetyl-D-mannosamine can be regulated in amammals in two different ways. In the liver, UDP-GlcNAc is epimerized to UDP-ManNAc by the bifunctional UDP-GlcNAc 2-epimerase, while in the kidney (mostly), GlcNAc is directly epimerized to ManNac by GlyNAc 2-epimerase Homo sapiens