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Literature summary extracted from

  • Kumari, A.; Kishor, N.; Guptasarma, P.
    Characterization of a mildly alkalophilic and thermostable recombinant Thermus thermophilus laccase with applications in decolourization of dyes (2018), Biotechnol. Lett., 40, 285-295 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.10.3.2 industry the enzyme decoloures up to six different industrial dyes, with or without the use of redox mediators such as ABTS Thermus thermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 overexpression in Escherichia coli Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.0153
-
 syringaldazine 40°C, pH 6.0 Thermus thermophilus
1.10.3.2 0.167
-
 syringaldazine 35°C, pH 6.0 Thermus thermophilus
1.10.3.2 0.256
-
 syringaldazine 30°C, pH 6.0 Thermus thermophilus
1.10.3.2 2.4
-
 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) 60°C, pH 6.0 Thermus thermophilus
1.10.3.2 3.8
-
 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) 55°C, pH 6.0 Thermus thermophilus
1.10.3.2 4.4
-
 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) 50°C, pH 6.0 Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 53000
-
 SDS-PAGE Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Thermus thermophilus Q72HW2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) + O2
-
 Thermus thermophilus ?
-
 ?
1.10.3.2 4-fluoro-2-methylphenol + O2
-
 Thermus thermophilus ?
-
 ?
1.10.3.2 additional information the purified enzyme is able to decolourize Green Dye, Orange Dye and Acid Red Dye. The enzyme is unable to decolourize NBB, RBB, or Congo Red. However, with 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) (5 mM) acting as redox mediator, the enzyme decolourizes NBB, RBB, or Congo Red Thermus thermophilus ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Thermus thermophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 5300, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 TthLAC
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 60
-
 reaction time: 180 min Thermus thermophilus
1.10.3.2 90
-
 reactions time: less than 1 h Thermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 40 85 40°C: about 40% of maximal activity, 85°C: about 55% of maximal activity Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 65
-
 the enzyme undergoes slow partial unfolding and thermal inactivation above 65°C, making it unsuitable for long incubations above this temperature Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 1.58
-
 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) 50°C, pH 6.0 Thermus thermophilus
1.10.3.2 3.33
-
 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) 55°C, pH 6.0 Thermus thermophilus
1.10.3.2 3.66
-
 syringaldazine 30°C, pH 6.0 Thermus thermophilus
1.10.3.2 4.77
-
 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonate) 60°C, pH 6.0 Thermus thermophilus
1.10.3.2 5.33
-
 syringaldazine 35°C, pH 6.0 Thermus thermophilus
1.10.3.2 10.3
-
 syringaldazine 40°C, pH 6.0 Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 6 8
-
 Thermus thermophilus