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Literature summary extracted from

  • Yeom, S.; Kim, B.; Park, C.; Oh, D.
    Substrate specificity of ribose-5-phosphate isomerases from Clostridium difficile and Thermotoga maritima (2010), Biotechnol. Lett., 32, 829-835 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.6
-
Clostridioides difficile
5.3.1.6
-
Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.6 additional information not inhibited by EDTA Clostridioides difficile
5.3.1.6 additional information not inhibited by EDTA Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.6 5 20 L-talose pH 7.5 and 40°C Clostridioides difficile
5.3.1.6 30
-
D-ribose 5-phosphate pH 7.5 and 40°C Clostridioides difficile
5.3.1.6 37
-
D-ribose 5-phosphate pH 8.0 and 70°C Thermotoga maritima
5.3.1.6 110
-
D-ribose pH 8.0 and 70°C Thermotoga maritima
5.3.1.6 130
-
D-allose pH 8.0 and 70°C Thermotoga maritima
5.3.1.6 190
-
L-talose pH 8.0 and 70°C Thermotoga maritima
5.3.1.6 270
-
D-ribose pH 7.5 and 40°C Clostridioides difficile
5.3.1.6 460
-
D-allose pH 7.5 and 40°C Clostridioides difficile

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.6 additional information not affected by metal ions Clostridioides difficile
5.3.1.6 additional information not effected by metal ions Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.6 34400
-
gel filtration Clostridioides difficile
5.3.1.6 67000
-
gel filtration Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.6 Clostridioides difficile AM180355
-
-
5.3.1.6 Clostridioides difficile ATCC BAA-1382D-5 AM180355
-
-
5.3.1.6 Thermotoga maritima Q9X0G9
-
-
5.3.1.6 Thermotoga maritima DSM 3109 Q9X0G9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.6 D-allose the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose Clostridioides difficile D-psicose
-
?
5.3.1.6 D-allose the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose Thermotoga maritima D-psicose
-
?
5.3.1.6 D-allose the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose Clostridioides difficile ATCC BAA-1382D-5 D-psicose
-
?
5.3.1.6 D-allose the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose Thermotoga maritima DSM 3109 D-psicose
-
?
5.3.1.6 D-gulose the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose Clostridioides difficile D-sorbose
-
?
5.3.1.6 D-ribose the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose Clostridioides difficile D-ribulose
-
?
5.3.1.6 D-ribose the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose Thermotoga maritima D-ribulose
-
?
5.3.1.6 D-ribose the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose Clostridioides difficile ATCC BAA-1382D-5 D-ribulose
-
?
5.3.1.6 D-ribose the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose Thermotoga maritima DSM 3109 D-ribulose
-
?
5.3.1.6 D-ribose 5-phosphate
-
Clostridioides difficile D-ribulose 5-phosphate
-
?
5.3.1.6 D-ribose 5-phosphate
-
Thermotoga maritima D-ribulose 5-phosphate
-
?
5.3.1.6 D-ribose 5-phosphate
-
Thermotoga maritima DSM 3109 D-ribulose 5-phosphate
-
?
5.3.1.6 D-talose the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose Thermotoga maritima D-tagatose
-
?
5.3.1.6 L-allose the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose Thermotoga maritima L-psicose
-
?
5.3.1.6 L-lyxose the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose Clostridioides difficile L-xylulose
-
?
5.3.1.6 L-lyxose the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose Thermotoga maritima L-xylulose
-
?
5.3.1.6 L-lyxose the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose Clostridioides difficile ATCC BAA-1382D-5 L-xylulose
-
?
5.3.1.6 L-lyxose the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose Thermotoga maritima DSM 3109 L-xylulose
-
?
5.3.1.6 L-Mannose the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose Clostridioides difficile L-Fructose
-
?
5.3.1.6 L-Mannose the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose Clostridioides difficile ATCC BAA-1382D-5 L-Fructose
-
?
5.3.1.6 L-ribose the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose Thermotoga maritima L-ribulose
-
?
5.3.1.6 L-talose the specific activity follows the order D-ribose, D-allose, L-talose, L-lyxose, D-gulose, L-mannose Clostridioides difficile L-tagatose
-
?
5.3.1.6 L-talose the specific activity follows the order L-talose, D-ribose, D-allose, L-allose, L-lyxose, L-ribose, D-tallose Thermotoga maritima L-tagatose
-
?
5.3.1.6 additional information the enzyme displays activity only with aldose substrates having hydroxyl groups configured in the same direction at the C2, C3, and C4 positions Thermotoga maritima ?
-
?
5.3.1.6 additional information the enzyme displays activity only with aldose substrates possessing hydroxyl groups oriented in the right-handed configuration (Fischer projections) at the C2 and C3 positions, such as D-ribose, D-allose, L-talose, L-lyxose, D-gulose, and L-mannose Clostridioides difficile ?
-
?
5.3.1.6 additional information the enzyme displays activity only with aldose substrates possessing hydroxyl groups oriented in the right-handed configuration (Fischer projections) at the C2 and C3 positions, such as D-ribose, D-allose, L-talose, L-lyxose, D-gulose, and L-mannose Clostridioides difficile ATCC BAA-1382D-5 ?
-
?
5.3.1.6 additional information the enzyme displays activity only with aldose substrates having hydroxyl groups configured in the same direction at the C2, C3, and C4 positions Thermotoga maritima DSM 3109 ?
-
?

Subunits

EC Number Subunits Comment Organism
5.3.1.6 dimer 2 * 17200, SDS-PAGE Clostridioides difficile
5.3.1.6 tetramer 2 * 16700, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
5.3.1.6 ribose-5-phosphate isomerase
-
Clostridioides difficile
5.3.1.6 ribose-5-phosphate isomerase
-
Thermotoga maritima
5.3.1.6 RpiB
-
Clostridioides difficile
5.3.1.6 RpiB
-
Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.6 40
-
substrate: D-ribose Clostridioides difficile
5.3.1.6 70
-
substrate: L-talose Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.6 55
-
half-life: 53 min Clostridioides difficile
5.3.1.6 75
-
half-life: 191 min Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.6 140
-
L-talose pH 7.5 and 40°C Clostridioides difficile
5.3.1.6 140
-
D-allose pH 8.0 and 70°C Thermotoga maritima
5.3.1.6 160
-
D-ribose pH 7.5 and 40°C Clostridioides difficile
5.3.1.6 200
-
D-allose pH 7.5 and 40°C Clostridioides difficile
5.3.1.6 500
-
D-ribose pH 8.0 and 70°C Thermotoga maritima
5.3.1.6 1340
-
L-talose pH 8.0 and 70°C Thermotoga maritima
5.3.1.6 15100
-
D-ribose 5-phosphate pH 7.5 and 40°C Clostridioides difficile
5.3.1.6 20000
-
D-ribose 5-phosphate pH 8.0 and 70°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.6 7.5
-
substrate: D-ribose Clostridioides difficile
5.3.1.6 8
-
substrate: L-talose Thermotoga maritima

pI Value

EC Number Organism Comment pI Value Maximum pI Value
5.3.1.6 Clostridioides difficile
-
-
6.4
5.3.1.6 Thermotoga maritima
-
-
7.2

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.6 0.3
-
L-talose pH 7.5 and 40°C Clostridioides difficile
5.3.1.6 0.4
-
D-allose pH 7.5 and 40°C Clostridioides difficile
5.3.1.6 0.6
-
D-ribose pH 7.5 and 40°C Clostridioides difficile
5.3.1.6 1.1
-
D-allose pH 8.0 and 70°C Thermotoga maritima
5.3.1.6 4.7
-
D-ribose pH 8.0 and 70°C Thermotoga maritima
5.3.1.6 7
-
L-talose pH 8.0 and 70°C Thermotoga maritima
5.3.1.6 500
-
D-ribose 5-phosphate pH 7.5 and 40°C Clostridioides difficile
5.3.1.6 540
-
D-ribose 5-phosphate pH 8.0 and 70°C Thermotoga maritima