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Literature summary extracted from

  • De Luca, V.; Vullo, D.; Del Prete, S.; Carginale, V.; Scozzafava, A.; Osman, S.M.; AlOthman, Z.; Supuran, C.T.; Capasso, C.
    Cloning, characterization and anion inhibition studies of a new gamma-carbonic anhydrase from the Antarctic bacterium Pseudoalteromonas haloplanktis (2015), Bioorg. Med. Chem., 23, 4405-4409 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.1 gene PhaCAgamma, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)(ArcticExpress DE3 competent cells), subcloning in Escherichia coli strain DH5alpha Pseudoalteromonas haloplanktis ANT/505

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 acetazolamide sulfonamide CA inhibitor (CAI) Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 B4O72-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 Br-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 CN-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 CNO-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 CO32-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 CS32-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 Et2NCS2-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 FSO3-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 H2NSO2NH2
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 H2NSO3H
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 HCO3-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 HS-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 HSO3-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 additional information no inhibition by fluoride, iodide, chloride, azide, sulfate, P2O74-, V2O74-, ReO4-, chlorate, tetrafluoroborate, PF6-, and CF3SO3- Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 NH(SO3)22-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 NO2-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 NO3-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 Ph-AsO3H2
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 Ph-B(OH)2
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 RuO4-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 S2O82-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 SCN-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 SeCN-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 SeO42-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 SnO32-
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 TeO42-
-
 Pseudoalteromonas haloplanktis ANT/505

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 CO2 + H2O Pseudoalteromonas haloplanktis ANT/505
-
 H2CO3
-
 r
4.2.1.1 H2CO3 Pseudoalteromonas haloplanktis ANT/505
-
 CO2 + H2O
-
 r
4.2.1.1 additional information Pseudoalteromonas haloplanktis ANT/505 the enzyme has a medium-low catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Pseudoalteromonas haloplanktis ANT/505 F3BJI2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)/ArcticExpress DE3 cells by nickel affinity chromatography Pseudoalteromonas haloplanktis ANT/505

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 CO2 + H2O
-
 Pseudoalteromonas haloplanktis ANT/505 H2CO3
-
 r
4.2.1.1 H2CO3
-
 Pseudoalteromonas haloplanktis ANT/505 CO2 + H2O
-
 r
4.2.1.1 additional information the enzyme has a medium-low catalytic activity for the physiologic reaction of CO2 hydration to bicarbonate and protons Pseudoalteromonas haloplanktis ANT/505 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 gamma-carbonic anhydrase
-
 Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 PhaCAgamma
-
 Pseudoalteromonas haloplanktis ANT/505

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.1 20
-
 assay at Pseudoalteromonas haloplanktis ANT/505

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.1 140000
-
 CO2 pH 8.3, 20°C Pseudoalteromonas haloplanktis ANT/505

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.1 8.3
-
 assay at Pseudoalteromonas haloplanktis ANT/505

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.1 0.000403
-
 acetazolamide pH 8.3, 20°C Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 0.082
-
 H2NSO2NH2 pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 0.087
-
 Ph-AsO3H2 pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 0.088
-
 Ph-B(OH)2 pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 0.091
-
 H2NSO3H pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 0.96
-
 Et2NCS2- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 1.7
-
 CNO- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 2.5
-
 CO32- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 5.6
-
 NO3- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 5.9
-
 CN- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 6.1
-
 SeO42- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 6.2
-
 HCO3- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 6.5
-
 NH(SO3)22- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 6.7
-
 NO2- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 6.7
-
 SnO32- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 7.7
-
 FSO3- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 7.8
-
 SeCN- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 8
-
 B4O72- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 8.4
-
 HS- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 8.5
-
 TeO42- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 8.7
-
 Br- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 8.7
-
 RuO4- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 9.1
-
 S2O82- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 9.3
-
 SCN- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 24.1
-
 HSO3- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505
4.2.1.1 34.6
-
 CS32- pH 8.3, 20°C, inhibition of CO2 hydration activity Pseudoalteromonas haloplanktis ANT/505

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.1 1900
-
 CO2 pH 8.3, 20°C Pseudoalteromonas haloplanktis ANT/505