EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.3.1 | expressed in Escherichia coli C41(DE3) cells | Mycobacterium tuberculosis |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.3.1 | E423A | inactive | Mycobacterium tuberculosis |
4.1.3.1 | E423A/E424A | inactive | Mycobacterium tuberculosis |
4.1.3.1 | E423D | the mutant shows about 75% of wild type enzyme activity | Mycobacterium tuberculosis |
4.1.3.1 | E423D/E424D | the mutant shows about 60% of wild type enzyme activity | Mycobacterium tuberculosis |
4.1.3.1 | E423K | inactive | Mycobacterium tuberculosis |
4.1.3.1 | E423K/E424K | inactive | Mycobacterium tuberculosis |
4.1.3.1 | E423L | inactive | Mycobacterium tuberculosis |
4.1.3.1 | E424A | inactive | Mycobacterium tuberculosis |
4.1.3.1 | E424D | the mutant shows about 70% of wild type enzyme activity | Mycobacterium tuberculosis |
4.1.3.1 | E424K | inactive | Mycobacterium tuberculosis |
4.1.3.1 | E424L | inactive | Mycobacterium tuberculosis |
4.1.3.1 | H180A | the mutation leads to the destabilization and formation of inactive monomeric enzyme | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.1 | 1.142 | - |
threo-DL-Isocitrate | wild type enzyme, at pH 7.5 and 30°C | Mycobacterium tuberculosis | |
4.1.3.1 | 1.423 | - |
threo-DL-Isocitrate | mutant enzyme E423D, at pH 7.5 and 30°C | Mycobacterium tuberculosis | |
4.1.3.1 | 1.861 | - |
threo-DL-Isocitrate | mutant enzyme E424D, at pH 7.5 and 30°C | Mycobacterium tuberculosis | |
4.1.3.1 | 2.031 | - |
threo-DL-Isocitrate | mutant enzyme E423D/E424D, at pH 7.5 and 30°C | Mycobacterium tuberculosis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.3.1 | 192000 | - |
gel filtration | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.1 | isocitrate | Mycobacterium tuberculosis | - |
succinate + glyoxylate | - |
? | |
4.1.3.1 | isocitrate | Mycobacterium tuberculosis H37Rv | - |
succinate + glyoxylate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.3.1 | Mycobacterium tuberculosis | P9WKK7 | - |
- |
4.1.3.1 | Mycobacterium tuberculosis H37Rv | P9WKK7 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.3.1 | Ni-NTA column chromatography | Mycobacterium tuberculosis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.3.1 | isocitrate | - |
Mycobacterium tuberculosis | succinate + glyoxylate | - |
? | |
4.1.3.1 | isocitrate | - |
Mycobacterium tuberculosis H37Rv | succinate + glyoxylate | - |
? | |
4.1.3.1 | threo-DL-isocitrate | - |
Mycobacterium tuberculosis | succinate + glyoxylate | - |
? | |
4.1.3.1 | threo-DL-isocitrate | - |
Mycobacterium tuberculosis H37Rv | succinate + glyoxylate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.3.1 | homotetramer | 4 * 48000, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.3.1 | ICL | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.3.1 | 7.53 | - |
threo-DL-Isocitrate | mutant enzyme E423D/E424D, at pH 7.5 and 30°C | Mycobacterium tuberculosis | |
4.1.3.1 | 8.34 | - |
threo-DL-Isocitrate | mutant enzyme E423D, at pH 7.5 and 30°C | Mycobacterium tuberculosis | |
4.1.3.1 | 8.46 | - |
threo-DL-Isocitrate | mutant enzyme E424D, at pH 7.5 and 30°C | Mycobacterium tuberculosis | |
4.1.3.1 | 9.78 | - |
threo-DL-Isocitrate | wild type enzyme, at pH 7.5 and 30°C | Mycobacterium tuberculosis |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.3.1 | physiological function | isocitrate lyase is a key enzyme of the glyoxylate shunt crucial for the survival of Mycobacterium tuberculosis in macrophages during persistent infection | Mycobacterium tuberculosis |