Literature summary extracted from

Hilario, E.; Caulkins, B.; Huang, Y.; You, W.; Chang, C.; Mueller, L.; Dunn, M.; Fan, L.
Visualizing the tunnel in tryptophan synthase with crystallography Insights into a selective filter for accommodating indole and rejecting water (2016), Biochim. Biophys. Acta, 1864, 268-279 .

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
4.2.1.20	structures of tryptophan synthase crystallized with varying numbers of amphipathic N-(4'-trifluoromethoxybenzoyl)-2-aminoethyl phosphate. One of the ligands threads into the tunnel from the beta-site and reveals a distinct hydrophobic region. The N-(4'-trifluoromethoxybenzoyl)-2-aminoethyl phosphate phosphoryl group fits into a polar pocket of the beta-subunit active site. One portion of the tunnel binds clusters of water molecules, whereas waters are not observed in the nonpolar N-(4'-trifluoromethoxybenzoyl)-2-aminoethyl phosphate binding region of the tunnel. Hydrophobic molecules can freely diffuse between the alpha- and beta-sites via the tunnel, while water does not. Exclusion of water may serve to inhibit reaction of water with the alpha-aminoacrylate intermediate to form ammonium ion and pyruvate	Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
4.2.1.20	N-(4'-trifluoromethoxybenzoyl)-2-aminoethyl phosphate	-	Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.2.1.20	Salmonella enterica subsp. enterica serovar Typhimurium	P00929	-	-
4.2.1.20	Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720	P00929	-	-

Synonyms

EC Number	Synonyms	Comment	Organism
4.2.1.20	TrpA	alpha-subinit	Salmonella enterica subsp. enterica serovar Typhimurium

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Release 2023.1 (January 2023)