EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.92 | CYP74C13_MT gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain Rosetta-gami(DE3)pLysS B | Glycine max |
4.2.1.92 | CYP74C13_MT gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS | Cucumis sativus |
4.2.1.92 | CYP74C13_MT gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain Rosetta-gami(DE3)pLysS B | Medicago truncatula |
4.2.1.92 | CYP74C1_CS gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type enzyme in Escherichia coli strain BL21(DE3)pLysS | Cucumis sativus |
4.2.1.92 | CYP74C4_ST gene, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged wild-type enzyme in Escherichia coli strain BL21(DE3)pLysS | Solanum tuberosum |
4.2.1.92 | sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Cucumis melo |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.92 | L93F/G283A | site-directed mutagenesis, the mutant shows altered substrate specificity and increased activity compared to the wild-type enzyme | Solanum tuberosum |
4.2.1.92 | L93F/G283A | site-directed mutagenesis, the mutant shows altered substrate specificity and increased activity compared to the wild-type enzyme. The mutant form preferentially utilise (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | Cucumis sativus |
4.2.1.92 | L97F | site-directed mutagenesis, L97F/TCFNSF, multiple mutations of the hydroperoxide-binding domain leading to the TCFNSF motif virtually do not alter the specificity of catalysis compared to the wild-type enzyme. Conversely, the L97F variant offers good allene oxide synthase (AOS) activity with (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid (9-HPOD). The mutant form preferentially utilise (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | Medicago truncatula |
4.2.1.92 | L97F | site-directed mutagenesis, the mutant shows altered substrate specificity and decreased activity compared to the wild-type enzyme | Solanum tuberosum |
4.2.1.92 | L98F/A287G | site-directed mutagenesis, the mutant shows altered substrate specificity and increased activity compared to the wild-type enzyme | Solanum tuberosum |
4.2.1.92 | L98F/A287G | site-directed mutagenesis, the mutant shows altered substrate specificity and increased activity compared to the wild-type enzyme. The mutant form preferentially utilise (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | Cucumis sativus |
4.2.1.92 | additional information | reaction products are identified and quantified using HPLC chromatography and mass spectrometry | Cucumis sativus |
4.2.1.92 | additional information | reaction products are identified and quantified using HPLC chromatography and mass spectrometry | Medicago truncatula |
4.2.1.92 | additional information | reaction products are identified and quantified using HPLC chromatography and mass spectrometry. All three mutant forms preferentially utilise (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid. Multiple mutations of the hydroperoxide-binding domain leading to the TCFNSF motif virtually did not alter the specificity of catalysis compared to the wild-type enzyme. Conversely, the L97F variant offers good allene oxide synthase (AOS) activity with (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid (9-HPOD) | Solanum tuberosum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.92 | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | Cucumis sativus | - |
(9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | Glycine max | - |
(9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | Solanum tuberosum | - |
(9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | Medicago truncatula | - |
(9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | Cucumis sativus | - |
(9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + 9-hydroxy-10-oxo-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + H2O + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | Cucumis melo | - |
(9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + 9-hydroxy-10-oxo-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + H2O + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | Cucumis sativus | - |
9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | Glycine max | - |
9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | Medicago truncatula | - |
9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | Solanum tuberosum | - |
(9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | Cucumis sativus | - |
(9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | Cucumis melo | - |
(9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | Solanum tuberosum | - |
(9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | Cucumis sativus | - |
11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | Glycine max | - |
11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | Cucumis melo | - |
11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | Medicago truncatula | - |
11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | Cucumis sativus | - |
(9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | Glycine max | - |
(9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | Cucumis melo | - |
(9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | Medicago truncatula | - |
(9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | additional information | Cucumis sativus | reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specificities, overview | ? | - |
? | |
4.2.1.92 | additional information | Cucumis melo | reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specificities, overview | ? | - |
? | |
4.2.1.92 | additional information | Glycine max | reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview | ? | - |
? | |
4.2.1.92 | additional information | Solanum tuberosum | reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview | ? | - |
? | |
4.2.1.92 | additional information | Cucumis sativus | reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview | ? | - |
? | |
4.2.1.92 | additional information | Medicago truncatula | reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.92 | Cucumis melo | Q93XR3 | - |
- |
4.2.1.92 | Cucumis sativus | A0A0A0K2U8 | - |
- |
4.2.1.92 | Cucumis sativus | U5YTM6 | - |
- |
4.2.1.92 | Glycine max | M4WFN0 | - |
- |
4.2.1.92 | Medicago truncatula | Q7X9B3 | - |
- |
4.2.1.92 | Solanum tuberosum | C7ENW3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.92 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography | Cucumis melo |
4.2.1.92 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography | Cucumis sativus |
4.2.1.92 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta-gami(DE3)pLysS B by nickel affinity chromatography | Medicago truncatula |
4.2.1.92 | recombinant His-tagged wild-type enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography | Cucumis sativus |
4.2.1.92 | recombinant His-tagged wild-type enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography | Solanum tuberosum |
4.2.1.92 | recombinant His-tagged wild-type enzyme from Escherichia coli strain Rosetta-gami(DE3)pLysS B by nickel affinity chromatography | Glycine max |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.92 | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | - |
Cucumis sativus | (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | - |
Glycine max | (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | - |
Solanum tuberosum | (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | - |
Medicago truncatula | (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | - |
Cucumis sativus | (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + 9-hydroxy-10-oxo-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + H2O + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | - |
Cucumis melo | (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acid + 9-hydroxy-10-oxo-12-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + H2O + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | - |
Cucumis sativus | 9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | - |
Glycine max | 9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | - |
Medicago truncatula | 9-hydroxynonanoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | - |
Solanum tuberosum | (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | - |
Cucumis sativus | (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | - |
Cucumis melo | (9Z)-12-hydroxy-9-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | - |
Solanum tuberosum | (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | - |
Cucumis sativus | 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | - |
Glycine max | 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | - |
Cucumis melo | 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | - |
Medicago truncatula | 11-hydroxy-12,13-epoxy-9-octadecenoic acid + 9-hydroxy-12,13-epoxy-10-octadecenoic acid + (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | - |
Cucumis sativus | (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | - |
Glycine max | (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | - |
Cucumis melo | (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | - |
Medicago truncatula | (9Z)-12-hydroxy-9-dodecenoic acid + (10E)-12-hydroxy-10-dodecenoic acid + ? | - |
? | |
4.2.1.92 | additional information | reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specificities, overview | Cucumis sativus | ? | - |
? | |
4.2.1.92 | additional information | reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specificities, overview | Cucumis melo | ? | - |
? | |
4.2.1.92 | additional information | reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview | Cucumis sativus | ? | - |
? | |
4.2.1.92 | additional information | reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview | Glycine max | ? | - |
? | |
4.2.1.92 | additional information | reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview | Solanum tuberosum | ? | - |
? | |
4.2.1.92 | additional information | reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview | Medicago truncatula | ? | - |
? | |
4.2.1.92 | additional information | no activity with (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | Solanum tuberosum | ? | - |
? | |
4.2.1.92 | additional information | reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview. No activity with (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | Solanum tuberosum | ? | - |
? | |
4.2.1.92 | additional information | reaction products are identified and quantified using HPLC chromatography and mass spectrometry, product specifities, overview. No activity with (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | Cucumis melo | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.92 | More | three-dimensional modelling of CYP74s and structure comparisons | Cucumis sativus |
4.2.1.92 | More | three-dimensional modelling of CYP74s and structure comparisons | Glycine max |
4.2.1.92 | More | three-dimensional modelling of CYP74s and structure comparisons | Solanum tuberosum |
4.2.1.92 | More | three-dimensional modelling of CYP74s and structure comparisons | Cucumis melo |
4.2.1.92 | More | three-dimensional modelling of CYP74s and structure comparisons | Medicago truncatula |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.92 | 9/13 hydroperoxide lyase | - |
Medicago truncatula |
4.2.1.92 | CmHPL | - |
Cucumis melo |
4.2.1.92 | Csa_7G075590 | - |
Cucumis sativus |
4.2.1.92 | CYP74C13_GM | - |
Glycine max |
4.2.1.92 | CYP74C13_MT | - |
Medicago truncatula |
4.2.1.92 | CYP74C1_CS | - |
Cucumis sativus |
4.2.1.92 | CYP74C2 | - |
Cucumis melo |
4.2.1.92 | CYP74C31 | - |
Cucumis sativus |
4.2.1.92 | CYP74C4_ST | - |
Solanum tuberosum |
4.2.1.92 | fatty acid 9-hydroperoxide lyase | UniProt | Cucumis sativus |
4.2.1.92 | fatty acid 9-hydroperoxide lyase | UniProt | Cucumis melo |
4.2.1.92 | fatty acid hydroperoxide lyase | UniProt | Solanum tuberosum |
4.2.1.92 | fatty acid hydroperoxide lyase | UniProt | Medicago truncatula |
4.2.1.92 | HPL | - |
Cucumis sativus |
4.2.1.92 | HPL | - |
Glycine max |
4.2.1.92 | HPL | - |
Solanum tuberosum |
4.2.1.92 | HPL | - |
Cucumis melo |
4.2.1.92 | HPL | - |
Medicago truncatula |
4.2.1.92 | HPL1 | - |
Cucumis sativus |
4.2.1.92 | HPL1 | - |
Medicago truncatula |
4.2.1.92 | hydroperoxide lyase | - |
Cucumis sativus |
4.2.1.92 | hydroperoxide lyase | - |
Glycine max |
4.2.1.92 | hydroperoxide lyase | - |
Solanum tuberosum |
4.2.1.92 | hydroperoxide lyase | - |
Cucumis melo |
4.2.1.92 | hydroperoxide lyase | - |
Medicago truncatula |
4.2.1.92 | MtrunA17_Chr4g0034141 | - |
Medicago truncatula |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.92 | 23 | - |
assay at | Cucumis sativus |
4.2.1.92 | 23 | - |
assay at | Glycine max |
4.2.1.92 | 23 | - |
assay at | Solanum tuberosum |
4.2.1.92 | 23 | - |
assay at | Cucumis melo |
4.2.1.92 | 23 | - |
assay at | Medicago truncatula |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.92 | 173.8 | - |
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Glycine max | |
4.2.1.92 | 187.1 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.0, 23°C, recombinant mutant L97F/TCFNSF enzyme | Medicago truncatula | |
4.2.1.92 | 228.6 | - |
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Glycine max | |
4.2.1.92 | 290 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Glycine max | |
4.2.1.92 | 337.6 | - |
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Cucumis sativus | |
4.2.1.92 | 356.1 | - |
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | pH 7.0, 23°C, recombinant mutant L98F/A287G enzyme | Cucumis sativus | |
4.2.1.92 | 375.4 | - |
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Cucumis sativus | |
4.2.1.92 | 377.2 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Cucumis sativus | |
4.2.1.92 | 455.3 | - |
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Cucumis sativus | |
4.2.1.92 | 471.4 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.0, 23°C, recombinant mutant L97F/TCFNSF enzyme | Medicago truncatula | |
4.2.1.92 | 530.9 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Cucumis sativus | |
4.2.1.92 | 550 | - |
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | pH 7.0, 23°C, recombinant mutant L97F/TCFNSF enzyme | Medicago truncatula | |
4.2.1.92 | 571.4 | - |
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | pH 7.0, 23°C, recombinant mutant L97F/TCFNSF enzyme | Medicago truncatula | |
4.2.1.92 | 596.4 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Cucumis sativus | |
4.2.1.92 | 600 | - |
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Solanum tuberosum | |
4.2.1.92 | 619.8 | - |
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | pH 7.0, 23°C, recombinant mutant L93F/G283A enzyme | Cucumis sativus | |
4.2.1.92 | 676.4 | - |
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Cucumis sativus | |
4.2.1.92 | 712.7 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.0, 23°C, recombinant mutant L93F/G283A enzyme | Cucumis sativus | |
4.2.1.92 | 732.4 | - |
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | pH 7.0, 23°C, recombinant mutant L93F/G283A enzyme | Cucumis sativus | |
4.2.1.92 | 752.4 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Cucumis sativus | |
4.2.1.92 | 768.9 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Medicago truncatula | |
4.2.1.92 | 773.3 | - |
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Medicago truncatula | |
4.2.1.92 | 829.1 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.0, 23°C, recombinant mutant L93F/G283A enzyme | Cucumis sativus | |
4.2.1.92 | 867.7 | - |
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Medicago truncatula | |
4.2.1.92 | 913.3 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Medicago truncatula | |
4.2.1.92 | 949.6 | - |
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | pH 7.0, 23°C, recombinant mutant L98F/A287G enzyme | Cucumis sativus | |
4.2.1.92 | 997 | - |
(9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid | pH 7.0, 23°C, recombinant mutant L98F/A287G enzyme | Cucumis sativus | |
4.2.1.92 | 1011.6 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Solanum tuberosum | |
4.2.1.92 | 1115.9 | - |
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoic acid | pH 7.0, 23°C, recombinant wild-type enzyme | Solanum tuberosum | |
4.2.1.92 | 1587.5 | - |
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid | pH 7.0, 23°C, recombinant mutant L98F/A287G enzyme | Cucumis sativus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.92 | 7 | - |
assay at, the reaction mixture acidifies to pH 6.0 | Cucumis sativus |
4.2.1.92 | 7 | - |
assay at, the reaction mixture acidifies to pH 6.0 | Glycine max |
4.2.1.92 | 7 | - |
assay at, the reaction mixture acidifies to pH 6.0 | Solanum tuberosum |
4.2.1.92 | 7 | - |
assay at, the reaction mixture acidifies to pH 6.0 | Cucumis melo |
4.2.1.92 | 7 | - |
assay at, the reaction mixture acidifies to pH 6.0 | Medicago truncatula |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.1.92 | evolution | hydroxyperoxide lyases belong to the CYP74C subfamily of fatty acid hydroperoxide transforming enzymes, unrooted phylogenetic tree of CYP74 family with special emphasis to CYP74C subfamily, overview | Cucumis sativus |
4.2.1.92 | evolution | hydroxyperoxide lyases belong to the CYP74C subfamily of fatty acid hydroperoxide transforming enzymes, unrooted phylogenetic tree of CYP74 family with special emphasis to CYP74C subfamily, overview | Glycine max |
4.2.1.92 | evolution | hydroxyperoxide lyases belong to the CYP74C subfamily of fatty acid hydroperoxide transforming enzymes, unrooted phylogenetic tree of CYP74 family with special emphasis to CYP74C subfamily, overview | Solanum tuberosum |
4.2.1.92 | evolution | hydroxyperoxide lyases belong to the CYP74C subfamily of fatty acid hydroperoxide transforming enzymes, unrooted phylogenetic tree of CYP74 family with special emphasis to CYP74C subfamily, overview | Cucumis melo |
4.2.1.92 | evolution | hydroxyperoxide lyases belong to the CYP74C subfamily of fatty acid hydroperoxide transforming enzymes, unrooted phylogenetic tree of CYP74 family with special emphasis to CYP74C subfamily, overview | Medicago truncatula |
4.2.1.92 | malfunction | the mutant enzyme preferentially utilises 9-hydroperoxylinoleic acid | Cucumis sativus |
4.2.1.92 | malfunction | the mutant enzyme preferentially utilises 9-hydroperoxylinoleic acid. Multiple mutations of the hydroperoxide-binding domain leading to the TCFNSF motif virtually do not alter the specificity of catalysis compared to the wild-type enzyme. Conversely, the L97F variant offers good allene oxide synthase (AOS) activity with 9-hydroperoxylinoleic acid (9-HPOD) | Medicago truncatula |
4.2.1.92 | additional information | three-dimensional modelling of CYP74s and structure comparisons | Cucumis sativus |
4.2.1.92 | additional information | three-dimensional modelling of CYP74s and structure comparisons | Glycine max |
4.2.1.92 | additional information | three-dimensional modelling of CYP74s and structure comparisons | Solanum tuberosum |
4.2.1.92 | additional information | three-dimensional modelling of CYP74s and structure comparisons | Cucumis melo |