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Literature summary extracted from

  • Guimaraes, S.L.; Coitinho, J.B.; Costa, D.M.; Araujo, S.S.; Whitman, C.P.; Nagem, R.A.
    Crystal structures of apo and liganded 4-oxalocrotonate decarboxylase uncover a structural basis for the metal-assisted decarboxylation of a vinylogous beta-keto acid (2016), Biochemistry, 55, 2632-2645 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.1.1.77 synthesis expression and purification of a monomeric, stable and active enzyme in the absence of vinylpyruvate hydratase Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.77
-
 Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.77 crystal structures for NahK in apo form and bonded with substrate analogues. A lid domain may be implicated in substrate binding Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.77 0.0049
-
 (3E)-2-oxohex-3-enedioate 25°C, pH 8.0 Pseudomonas putida
4.1.1.77 0.0199
-
 (3E)-2-oxohex-3-enedioate 25°C, pH 7.4 Pseudomonas putida
4.1.1.77 0.0222
-
 (3E)-2-oxohex-3-enedioate 25°C, pH 7.0 Pseudomonas putida
4.1.1.77 0.0517
-
 (3E)-2-oxohex-3-enedioate 25°C, pH 6.0 Pseudomonas putida
4.1.1.77 0.0647
-
 (3E)-2-oxohex-3-enedioate 25°C, pH 6.5 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.77 Mg2+ assay in presence of 5 mM Mg2+ Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.77 25000 30000 gel filtration Pseudomonas putida
4.1.1.77 26000
-
 dynamic light scattering Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.77 Pseudomonas putida Q1XGK3
-
-
4.1.1.77 Pseudomonas putida G7 Q1XGK3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.77 expression and purification of a monomeric, stable and active 4-OD in the absence of vinylpyruvate hydratase Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.77 (3E)-2-oxohex-3-enedioate
-
 Pseudomonas putida 2-oxopent-4-enoate + CO2
-
 ?
4.1.1.77 (3E)-2-oxohex-3-enedioate
-
 Pseudomonas putida G7 2-oxopent-4-enoate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.77 monomer 1 * 28400, calculated from sequence Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
4.1.1.77 NahK
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.77 21.8
-
 (3E)-2-oxohex-3-enedioate 25°C, pH 8.0 Pseudomonas putida
4.1.1.77 22.5
-
 (3E)-2-oxohex-3-enedioate 25°C, pH 6.0 Pseudomonas putida
4.1.1.77 98.8
-
 (3E)-2-oxohex-3-enedioate 25°C, pH 7.4 Pseudomonas putida
4.1.1.77 104.2
-
 (3E)-2-oxohex-3-enedioate 25°C, pH 7.0 Pseudomonas putida
4.1.1.77 108.4
-
 (3E)-2-oxohex-3-enedioate 25°C, pH 6.5 Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.77 440
-
 (3E)-2-oxohex-3-enedioate 25°C, pH 6.0 Pseudomonas putida
4.1.1.77 1700
-
 (3E)-2-oxohex-3-enedioate 25°C, pH 6.5 Pseudomonas putida
4.1.1.77 4500
-
 (3E)-2-oxohex-3-enedioate 25°C, pH 8.0 Pseudomonas putida
4.1.1.77 4700
-
 (3E)-2-oxohex-3-enedioate 25°C, pH 7.0 Pseudomonas putida
4.1.1.77 5000
-
 (3E)-2-oxohex-3-enedioate 25°C, pH 7.4 Pseudomonas putida