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Literature summary extracted from

  • Deka, G.; Bharath, S.R.; Savithri, H.S.; Murthy, M.R.N.
    Structural and biochemical studies on the role of active site Thr166 and Asp236 in the catalytic function of D-Serine deaminase from Salmonella typhimurium (2018), Biochem. Biophys. Res. Commun., 504, 40-45 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.18 cloned in pET21b vector, expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.1.18 hanging drop method at 25°C, 2.7 A resolution crystal structure of mutant enzyme T166A with bound pyridoxal 5'-phosphate Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

EC Number Protein Variants Comment Organism
4.3.1.18 D236L compared to wild-type enzyme and to mutant enzyme T166A the D236L mutant has a lower affinity for pyridoxal 5'-phosphate Salmonella enterica subsp. enterica serovar Typhimurium
4.3.1.18 T166A in apo-wild-type enzyme and in holo-mutant enzyme T166A structures, which are in the open conformation, Thr166 is about 8-9 A away from a modelled pyridoxal 5'-phosphate D-Ser-external aldimine complex. This distance is too large to function as a proton abstracting residue. Thr166 is important for the proton abstraction step of catalysis Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.18 49000
-
 x * 49000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.18 Salmonella enterica subsp. enterica serovar Typhimurium Q8ZL08
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.18
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.18 D-serine
-
 Salmonella enterica subsp. enterica serovar Typhimurium pyruvate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.1.18 ? x * 49000, SDS-PAGE, mutant enzymes T166A and D236L Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
4.3.1.18 D-Serine deaminase
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.18 7.5
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
4.3.1.18 pyridoxal 5'-phosphate dependent on Salmonella enterica subsp. enterica serovar Typhimurium