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Literature summary extracted from

  • Li, S.; Lou, X.; Xu, Y.; Teng, X.; Che, S.; Liu, R.; Bartlam, M.
    Crystal structure of a glutamate-1-semialdehyde-aminomutase from Pseudomonas aeruginosa PAO1 (2018), Biochem. Biophys. Res. Commun., 500, 804-809 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.3.8 gene pa4088, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.3.8 purified recombinant His-tagged wild-type enzyme and enzyme mutant K286A in complex with pyridoxamine 5'-phosphate cofactor, mixing of 10 mg/ml protein in 20 mM Tris, 200 mM NaCl, pH 8.0, with an equal volume of reservoir solution consisting of 0.2M ammonium sulfate, 0.1 M Bis-Tris, pH 6.5, and 25% PEG 3350 for the wild-type enzyme, and of 0.1 M Tris, pH 8.5, and 25% PEG3350 for the mutant enzyme, 20°C, 1 week, X-ray diffraction structure determination and analysis at 1.44-1.57 A resolution Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
5.4.3.8 K286A site-directed mutagenesis Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.3.8 L-glutamate 1-semialdehyde Pseudomonas aeruginosa
-
 5-aminolevulinate
-
 ?
5.4.3.8 L-glutamate 1-semialdehyde Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1
-
 5-aminolevulinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.3.8 Pseudomonas aeruginosa Q9HWU0
-
-
5.4.3.8 Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 Q9HWU0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.3.8 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.3.8 L-glutamate 1-semialdehyde
-
 Pseudomonas aeruginosa 5-aminolevulinate
-
 ?
5.4.3.8 L-glutamate 1-semialdehyde
-
 Pseudomonas aeruginosa ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1 5-aminolevulinate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.3.8 dimer three-dimensional structure analysis of wild-type and mutant enzymes, overview Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
5.4.3.8 glutamate-1-semialdehyde-aminomutase
-
 Pseudomonas aeruginosa
5.4.3.8 PA4088
-
 Pseudomonas aeruginosa
5.4.3.8 protein PA4088
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.3.8 additional information in both the pyridoxamine 5'-phosphate- and pyridoxal 5'-phosphate-bound structures, the gating loops are well-defined in electron density and are in the ordered open conformation. The conformation of the gating loop is symmetrical Pseudomonas aeruginosa
5.4.3.8 pyridoxal 5'-phosphate a pyridoxamine 5'-phosphate (PMP)/pyridoxal 5'-phosphate (PLP)-dependent enzyme, binding structure with a Schiff base linkage between the cofactor and the epsilon-amino group of Lys286, overview Pseudomonas aeruginosa
5.4.3.8 pyridoxamine 5'-phosphate a pyridoxamine 5'-phosphate (PMP)/pyridoxal 5'-phosphate (PLP)-dependent enzyme, binding structure, overview Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
5.4.3.8 evolution the enzyme is a member of the GSAM family Pseudomonas aeruginosa
5.4.3.8 additional information three-dimensional structure analysis of wild-type and mutant enzymes, overview. In both the pyridoxamine 5'-phosphate- and pyridoxal 5'-phosphate-bound structures, the gating loops are well-defined in electron density and are in the ordered open conformation. The conformation of the gating loop is symmetrical Pseudomonas aeruginosa