BRENDA - Enzyme Database show

Molecular structure of a prostaglandin D synthase requiring glutathione from the brown planthopper, Nilaparvata lugens

Yamamoto, K.; Higashiura, A.; Suzuki, M.; Aritake, K.; Urade, Y.; Nakagawa, A.; Biochem. Biophys. Res. Commun. 492, 166-171 (2017)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.2
glutathione transferase
-
Nilaparvata lugens
Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.2
expressed in Escherichia coli Rosetta (DE3) pLysS cells
Nilaparvata lugens
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.3.99.2
in complex with glutathione, sitting drop vapor diffusion method, using 0.1 M Tris-HCl, pH 8.0, 28% (w/v) PEG4000
Nilaparvata lugens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.2
0.32
-
GSH
at pH 8.0, temperature not specified in the publication
Nilaparvata lugens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
Prostaglandin H2
Nilaparvata lugens
-
Prostaglandin D2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.2
Nilaparvata lugens
J9Q529
strain Izumo
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.2
DEAE-Sepharose column chromatography and Superdex 200 gel filtration
Nilaparvata lugens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
1-chloro-2,4-dinitrobenzene + GSH
-
746994
Nilaparvata lugens
?
-
-
-
?
5.3.99.2
additional information
the enzyme is inactive toward 1,2-epoxy-3-(4-nitrophenoxy)-propane, 4-nitrophenethyl bromide, 4-nitrobenzyl chloride, 4-hydroxynonenal, ethacrynic acid, and 4-nitrophenyl acetate
746994
Nilaparvata lugens
?
-
-
-
-
5.3.99.2
Prostaglandin H2
-
746994
Nilaparvata lugens
Prostaglandin D2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
5.3.99.2
homodimer
2 * 23000, SDS-PAGE
Nilaparvata lugens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.99.2
8
-
-
Nilaparvata lugens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.2
glutathione transferase
-
Nilaparvata lugens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
expressed in Escherichia coli Rosetta (DE3) pLysS cells
Nilaparvata lugens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.3.99.2
in complex with glutathione, sitting drop vapor diffusion method, using 0.1 M Tris-HCl, pH 8.0, 28% (w/v) PEG4000
Nilaparvata lugens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.2
0.32
-
GSH
at pH 8.0, temperature not specified in the publication
Nilaparvata lugens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.2
Prostaglandin H2
Nilaparvata lugens
-
Prostaglandin D2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.2
DEAE-Sepharose column chromatography and Superdex 200 gel filtration
Nilaparvata lugens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.2
1-chloro-2,4-dinitrobenzene + GSH
-
746994
Nilaparvata lugens
?
-
-
-
?
5.3.99.2
additional information
the enzyme is inactive toward 1,2-epoxy-3-(4-nitrophenoxy)-propane, 4-nitrophenethyl bromide, 4-nitrobenzyl chloride, 4-hydroxynonenal, ethacrynic acid, and 4-nitrophenyl acetate
746994
Nilaparvata lugens
?
-
-
-
-
5.3.99.2
Prostaglandin H2
-
746994
Nilaparvata lugens
Prostaglandin D2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.99.2
homodimer
2 * 23000, SDS-PAGE
Nilaparvata lugens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.3.99.2
8
-
-
Nilaparvata lugens
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
5.3.99.2
1.7
-
GSH
at pH 8.0, temperature not specified in the publication
Nilaparvata lugens
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
5.3.99.2
1.7
-
GSH
at pH 8.0, temperature not specified in the publication
Nilaparvata lugens