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Literature summary extracted from

  • Deka, G.; Bharath, S.R.; Savithri, H.S.; Murthy, M.R.N.
    Structural studies on the decameric S. typhimurium arginine decarboxylase (ADC) Pyridoxal 5-phosphate binding induces conformational changes (2017), Biochem. Biophys. Res. Commun., 490, 1362-1368 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.19 gene adiA, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.19 purified recombinant His6-tagged apoenzyme without cofactor, microbatch method, mixing of 0.002 ml of 10 mg/ml protein in 25 mM Tris, pH 8.0, and 50 mM NaCl, with 0.002 ml of crystallization solution containing 2.0 M NaCl, 10% PEG 8000, and 25 mM Tris, pH 8.0, and placing under a layer of paraffin and silicone oils (1:1), 20°C, X-ray diffraction structure determination and analysis at 3.1 A resolution, molecular replacement using a polyalanine chain of the Escherichia coli enzyme, PDB ID 2VYC, as search model Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.19 800000
-
 about, recombinant enzyme, crystal structure Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.19 L-arginine Salmonella enterica subsp. enterica serovar Typhimurium
-
 agmatine + CO2
-
 ?
4.1.1.19 L-arginine Salmonella enterica subsp. enterica serovar Typhimurium 4/74
-
 agmatine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.19 Salmonella enterica subsp. enterica serovar Typhimurium E8X9U7
-
-
4.1.1.19 Salmonella enterica subsp. enterica serovar Typhimurium 4/74 E8X9U7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.19 recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration, to homogeneity Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.19 L-arginine
-
 Salmonella enterica subsp. enterica serovar Typhimurium agmatine + CO2
-
 ?
4.1.1.19 L-arginine
-
 Salmonella enterica subsp. enterica serovar Typhimurium 4/74 agmatine + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.19 decamer (alphabeta)5, 10 * 80000, about, recombinant His6-tagged enzyme, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
4.1.1.19 More a pentamer of dimers, structure of dimer and decamer, overview. unlike the interactions between 5fold related protomers, interactions that stabilize the dimeric structure are not pH-dependent Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
4.1.1.19 ADC
-
 Salmonella enterica subsp. enterica serovar Typhimurium
4.1.1.19 AdiA
-
 Salmonella enterica subsp. enterica serovar Typhimurium
4.1.1.19 StADC
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.19 pyridoxal 5'-phosphate PLP, the cofactor binding at the active site induces conformational changes in the enzyme, pyridoxal 5'-phosphate binding is important for substrate binding, the substrate might bind to ADC only after PLP binding Salmonella enterica subsp. enterica serovar Typhimurium

General Information

EC Number General Information Comment Organism
4.1.1.19 additional information the cofactor binding at the active site induces conformational changes in the enzyme, pyridoxal 5'-phosphate binding is important for substrate binding, the substrate might bind to ADC only after pyridoxal 5'-phosphate binding Salmonella enterica subsp. enterica serovar Typhimurium