EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.119 | 8-hydroxyquinoline | 1 mM, 54% inhibition | Xanthobacter flavus | |
1.3.1.119 | HgCl2 | 0.1 mM, complete loss of activity, most potent inhibitor | Xanthobacter flavus | |
1.3.1.119 | KCN | 1 mM, 26% inhibition | Xanthobacter flavus | |
1.3.1.119 | additional information | no inhibition by EDTA, 2,2-dipyridyl, and 1,10-phenanthroline | Xanthobacter flavus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.119 | 0.005 | - |
NAD+ | pH 8.0, 30°C, cosubstrate: 3,6-dichlorobenzene cis-dihydrodiol | Xanthobacter flavus | |
1.3.1.119 | 0.02 | - |
NAD+ | pH 8.0, 30°C, cosubstrate: benzene cis-dihydrodiol | Xanthobacter flavus | |
1.3.1.119 | 0.21 | - |
3,6-dichlorobenzene cis-dihydrodiol | pH 8.0, 30°C | Xanthobacter flavus | |
1.3.1.119 | 0.78 | - |
benzene cis-dihydrodiol | pH 8.0, 30°C | Xanthobacter flavus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.1.119 | 110000 | - |
gel filtration | Xanthobacter flavus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.119 | Xanthobacter flavus | - |
- |
- |
1.3.1.119 | Xanthobacter flavus DSM 10330 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.119 | - |
Xanthobacter flavus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.1.119 | 32 | - |
substrate: 3,6-dichlorobenzene cis-dihydrodiol, pH 8.0, 30°C | Xanthobacter flavus |
1.3.1.119 | 63 | - |
substrate: benzene cis-dihydrodiol, pH 8.0, 30°C | Xanthobacter flavus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.119 | 3,4-dichlorobenzene cis-dihydrodiol + NAD+ | activity is 30% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus | 3,4-dichlorocatechol + NADH + H+ | - |
? | |
1.3.1.119 | 3,4-dichlorobenzene cis-dihydrodiol + NAD+ | activity is 30% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus DSM 10330 | 3,4-dichlorocatechol + NADH + H+ | - |
? | |
1.3.1.119 | 3,5-dichlorobenzene cis-dihydrodiol + NAD+ | activity is 195% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus | 3,5-dichlorocatechol + NADH + H+ | - |
? | |
1.3.1.119 | 3,5-dichlorobenzene cis-dihydrodiol + NAD+ | activity is 195% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus DSM 10330 | 3,5-dichlorocatechol + NADH + H+ | - |
? | |
1.3.1.119 | 3,6-dichlorobenzene cis-dihydrodiol + NAD+ | activity is 60% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus | 3,6-dichlorocatechol + NADH + H+ | - |
? | |
1.3.1.119 | 3,6-dichlorobenzene cis-dihydrodiol + NAD+ | activity is 60% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus DSM 10330 | 3,6-dichlorocatechol + NADH + H+ | - |
? | |
1.3.1.119 | 3,6-dimethylbenzene cis-dihydrodiol + NAD+ | activity is 105% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus | 3,6-dimethylcatechol + NADH + H+ | - |
? | |
1.3.1.119 | 3,6-dimethylbenzene cis-dihydrodiol + NAD+ | activity is 105% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus DSM 10330 | 3,6-dimethylcatechol + NADH + H+ | - |
? | |
1.3.1.119 | 3-chlorobenzene cis-dihydrodiol + NAD+ | activity is 170% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus | 3-chlorocatechol + NADH + H+ | - |
? | |
1.3.1.119 | 3-chlorobenzene cis-dihydrodiol + NAD+ | activity is 170% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus DSM 10330 | 3-chlorocatechol + NADH + H+ | - |
? | |
1.3.1.119 | 3-fluorobenzene cis-dihydrodiol + NAD+ | activity is 145% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus | 3-fluorocatechol + NADH + H+ | - |
? | |
1.3.1.119 | 3-methylbenzene cis-dihydrodiol + NAD+ | activity is 185% compared to the activity with benzene cis-dihydrodiol | Xanthobacter flavus | 3-methylcatechol + NADH + H+ | - |
? | |
1.3.1.119 | benzene cis-dihydrodiol + NAD+ | - |
Xanthobacter flavus | catechol + NADH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.119 | homotetramer | 4 * 26500, SDS-PAGE | Xanthobacter flavus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.119 | 3,6-dichlorobenzene DDH | - |
Xanthobacter flavus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.119 | 14.5 | - |
3,6-dichlorobenzene cis-dihydrodiol | pH 8.0, 30°C | Xanthobacter flavus | |
1.3.1.119 | 14.5 | - |
NAD+ | pH 8.0, 30°C, cosubstrate: 3,6-dichlorobenzene cis-dihydrodiol | Xanthobacter flavus | |
1.3.1.119 | 27.8 | - |
benzene cis-dihydrodiol | pH 8.0, 30°C | Xanthobacter flavus | |
1.3.1.119 | 27.8 | - |
NAD+ | pH 8.0, 30°C, cosubstrate: benzene cis-dihydrodiol | Xanthobacter flavus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.119 | 6 | - |
optimum stability at 4°C | Xanthobacter flavus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.119 | NAD+ | the enzyme accepts also NADP+ as cosubstrate. The activity with NADP+ is reduced to 14% of that with NAD+ | Xanthobacter flavus | |
1.3.1.119 | NADP+ | the activity with NADP+ is reduced to 14% of that with NAD+ | Xanthobacter flavus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
1.3.1.119 | Xanthobacter flavus | - |
- |
5.4 |