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Literature summary extracted from

  • Lu, J.; Brigham, C.J.; Plassmeier, J.K.; Sinskey, A.J.
    Characterization and modification of enzymes in the 2-ketoisovalerate biosynthesis pathway of Ralstonia eutropha H16 (2015), Appl. Microbiol. Biotechnol., 99, 761-774 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.9 expression in Escherichia coli Cupriavidus necator

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.9 L-leucine
-
 Cupriavidus necator
4.2.1.9 L-valine
-
 Cupriavidus necator

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.9 2.7
-
 2,3-dihydroxyisovalerate pH 7.0, 30°C Cupriavidus necator

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.9 Cupriavidus necator Q0K7F8
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.9 3.4
-
 pH 7.0, 30°C Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.9 2,3-dihydroxyisovalerate
-
 Cupriavidus necator 2-oxoisovalerate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.9 DHAD
-
 Cupriavidus necator
4.2.1.9 H16_A2987 locus name Cupriavidus necator
4.2.1.9 IlvD
-
 Cupriavidus necator

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.9 128
-
 pH 7.0, 30°C Cupriavidus necator L-leucine
4.2.1.9 131
-
 pH 7.0, 30°C Cupriavidus necator L-valine