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Literature summary extracted from

  • Petrareanu, G.; Balasu, M.; Vacaru, A.; Munteanu, C.; Ionescu, A.; Matei, I.; Szedlacsek, S.
    Phosphoketolases from Lactococcus lactis, Leuconostoc mesenteroides and Pseudomonas aeruginosa Dissimilar sequences, similar substrates but distinct enzymatic characteristics (2014), Appl. Microbiol. Biotechnol., 98, 7855-7867 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.9 cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.9 cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli Pseudomonas aeruginosa
4.1.2.9 cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli Lactococcus lactis subsp. lactis
4.1.2.22 cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.22 cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli Pseudomonas aeruginosa
4.1.2.22 cloned in a prokaryotic vector, and the encoded protein is expressed in Escherichia coli Lactococcus lactis subsp. lactis
4.1.2.22 expressed in Escherichia coli BL21(DE3)RIL cells Pseudomonas aeruginosa
4.1.2.22 expressed in Escherichia coli BL21(DE3)RIL cells Lactococcus lactis
4.1.2.22 expressed in Escherichia coli BL21(DE3)RIL cells Leuconostoc mesenteroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.9 0.035
-
D-xylulose 5-phosphate pH 6.5, 37°C Pseudomonas aeruginosa
4.1.2.9 1.76
-
D-fructose 6-phosphate pH 6.5, 37°C Pseudomonas aeruginosa
4.1.2.9 2.02
-
D-xylulose 5-phosphate pH 6.5, 37°C Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.9 3.04
-
D-xylulose 5-phosphate pH 6.5, 37°C Lactococcus lactis subsp. lactis
4.1.2.9 9.89
-
D-fructose 6-phosphate pH 6.5, 37°C Lactococcus lactis subsp. lactis
4.1.2.9 45.96
-
D-fructose 6-phosphate pH 6.5, 37°C Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.22 0.035
-
D-xylulose 5-phosphate pH 6.5, 37°C Pseudomonas aeruginosa
4.1.2.22 0.035
-
D-xylulose 5-phosphate at pH 6.5 and 37°C Pseudomonas aeruginosa
4.1.2.22 1.76
-
D-fructose 6-phosphate pH 6.5, 37°C Pseudomonas aeruginosa
4.1.2.22 1.76
-
D-fructose 6-phosphate at pH 6.5 and 37°C Pseudomonas aeruginosa
4.1.2.22 2.02
-
D-xylulose 5-phosphate pH 6.5, 37°C Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.22 2.02
-
D-xylulose 5-phosphate at pH 6.5 and 37°C Leuconostoc mesenteroides
4.1.2.22 3.04
-
D-xylulose 5-phosphate pH 6.5, 37°C Lactococcus lactis subsp. lactis
4.1.2.22 3.04
-
D-xylulose 5-phosphate at pH 6.5 and 37°C Lactococcus lactis
4.1.2.22 9.89
-
D-fructose 6-phosphate pH 6.5, 37°C Lactococcus lactis subsp. lactis
4.1.2.22 9.89
-
D-fructose 6-phosphate at pH 6.5 and 37°C Lactococcus lactis
4.1.2.22 45.96
-
D-fructose 6-phosphate pH 6.5, 37°C Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.22 45.96
-
D-fructose 6-phosphate at pH 6.5 and 37°C Leuconostoc mesenteroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.22 D-Fructose 6-phosphate + phosphate Pseudomonas aeruginosa
-
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
?
4.1.2.22 D-Fructose 6-phosphate + phosphate Lactococcus lactis
-
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
?
4.1.2.22 D-Fructose 6-phosphate + phosphate Leuconostoc mesenteroides
-
Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
?
4.1.2.22 D-Xylulose 5-phosphate + phosphate Pseudomonas aeruginosa preferred substrate Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?
4.1.2.22 D-Xylulose 5-phosphate + phosphate Lactococcus lactis preferred substrate Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?
4.1.2.22 D-Xylulose 5-phosphate + phosphate Leuconostoc mesenteroides preferred substrate Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.9 Lactococcus lactis subsp. lactis Q9CFH4
-
-
4.1.2.9 Lactococcus lactis subsp. lactis IL1403 Q9CFH4
-
-
4.1.2.9 Leuconostoc mesenteroides subsp. mesenteroides
-
-
-
4.1.2.9 Leuconostoc mesenteroides subsp. mesenteroides J18
-
-
-
4.1.2.9 Pseudomonas aeruginosa Q9HY13
-
-
4.1.2.22 Lactococcus lactis
-
-
-
4.1.2.22 Lactococcus lactis subsp. lactis Q9CFH4
-
-
4.1.2.22 Lactococcus lactis subsp. lactis IL1403 Q9CFH4
-
-
4.1.2.22 Leuconostoc mesenteroides
-
-
-
4.1.2.22 Leuconostoc mesenteroides subsp. mesenteroides
-
-
-
4.1.2.22 Leuconostoc mesenteroides subsp. mesenteroides J18
-
-
-
4.1.2.22 Pseudomonas aeruginosa
-
-
-
4.1.2.22 Pseudomonas aeruginosa Q9HY13
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.9
-
Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.9
-
Pseudomonas aeruginosa
4.1.2.9
-
Lactococcus lactis subsp. lactis
4.1.2.22
-
Pseudomonas aeruginosa
4.1.2.22
-
Lactococcus lactis
4.1.2.22
-
Leuconostoc mesenteroides
4.1.2.22
-
Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.22
-
Lactococcus lactis subsp. lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.9 D-Fructose 6-phosphate + phosphate the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate Pseudomonas aeruginosa Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
?
4.1.2.9 D-Fructose 6-phosphate + phosphate the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate Lactococcus lactis subsp. lactis Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
?
4.1.2.9 D-Fructose 6-phosphate + phosphate the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate Leuconostoc mesenteroides subsp. mesenteroides Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
?
4.1.2.9 D-Fructose 6-phosphate + phosphate the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate Lactococcus lactis subsp. lactis IL1403 Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
?
4.1.2.9 D-Fructose 6-phosphate + phosphate the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate Leuconostoc mesenteroides subsp. mesenteroides J18 Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
?
4.1.2.9 D-Xylulose 5-phosphate + phosphate the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate Pseudomonas aeruginosa Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?
4.1.2.9 D-Xylulose 5-phosphate + phosphate the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate Lactococcus lactis subsp. lactis Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?
4.1.2.9 D-Xylulose 5-phosphate + phosphate the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate Leuconostoc mesenteroides subsp. mesenteroides Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?
4.1.2.9 D-Xylulose 5-phosphate + phosphate the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate Lactococcus lactis subsp. lactis IL1403 Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?
4.1.2.9 D-Xylulose 5-phosphate + phosphate the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate Leuconostoc mesenteroides subsp. mesenteroides J18 Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?
4.1.2.22 D-Fructose 6-phosphate + phosphate
-
Pseudomonas aeruginosa Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
?
4.1.2.22 D-Fructose 6-phosphate + phosphate
-
Lactococcus lactis Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
?
4.1.2.22 D-Fructose 6-phosphate + phosphate
-
Leuconostoc mesenteroides Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
?
4.1.2.22 D-Fructose 6-phosphate + phosphate the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate Lactococcus lactis subsp. lactis Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
?
4.1.2.22 D-Fructose 6-phosphate + phosphate the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate Leuconostoc mesenteroides subsp. mesenteroides Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
?
4.1.2.22 D-Fructose 6-phosphate + phosphate the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate Pseudomonas aeruginosa Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
?
4.1.2.22 D-Fructose 6-phosphate + phosphate the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate Lactococcus lactis subsp. lactis IL1403 Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
?
4.1.2.22 D-Fructose 6-phosphate + phosphate the bifunctional enzyme shows activity with fructose-6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for fructose-6-phosphate Leuconostoc mesenteroides subsp. mesenteroides J18 Acetyl phosphate + D-erythrose 4-phosphate + H2O
-
?
4.1.2.22 D-Xylulose 5-phosphate + phosphate preferred substrate Pseudomonas aeruginosa Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?
4.1.2.22 D-Xylulose 5-phosphate + phosphate preferred substrate Lactococcus lactis Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?
4.1.2.22 D-Xylulose 5-phosphate + phosphate preferred substrate Leuconostoc mesenteroides Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?
4.1.2.22 D-Xylulose 5-phosphate + phosphate the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate Leuconostoc mesenteroides subsp. mesenteroides Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?
4.1.2.22 D-Xylulose 5-phosphate + phosphate the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate Lactococcus lactis subsp. lactis Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?
4.1.2.22 D-Xylulose 5-phosphate + phosphate the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose6-phosphate Pseudomonas aeruginosa Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?
4.1.2.22 D-Xylulose 5-phosphate + phosphate the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate Lactococcus lactis subsp. lactis IL1403 Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?
4.1.2.22 D-Xylulose 5-phosphate + phosphate the bifunctional enzyme shows activity with D-fructose 6-phosphate and D-xylulose 5-phosphate. It displays substantially higher preference for D-xylulose 5-phosphate than for D-fructose 6-phosphate Leuconostoc mesenteroides subsp. mesenteroides J18 Acetyl phosphate + D-glyceraldehyde 3-phosphate + H2O
-
?

Subunits

EC Number Subunits Comment Organism
4.1.2.22 ? x * 89200, SDS-PAGE Pseudomonas aeruginosa
4.1.2.22 ? x * 91858, calculated from amino acid sequence Leuconostoc mesenteroides
4.1.2.22 ? x * 94200, SDS-PAGE Leuconostoc mesenteroides
4.1.2.22 ? x * 95700, SDS-PAGE Lactococcus lactis

Synonyms

EC Number Synonyms Comment Organism
4.1.2.22 X5P/F6P PK
-
Pseudomonas aeruginosa
4.1.2.22 X5P/F6P PK
-
Lactococcus lactis
4.1.2.22 X5P/F6P PK
-
Leuconostoc mesenteroides
4.1.2.22 XFPK
-
Pseudomonas aeruginosa
4.1.2.22 XFPK
-
Lactococcus lactis
4.1.2.22 XFPK
-
Leuconostoc mesenteroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.22 37
-
-
Pseudomonas aeruginosa
4.1.2.22 37
-
-
Lactococcus lactis
4.1.2.22 37
-
-
Leuconostoc mesenteroides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.2.22 30 40 the activity sharply decreases at 30 and 40°C to approximately 60% of the maximum specific activity observed at 37°C Pseudomonas aeruginosa
4.1.2.22 30 40 the activity sharply decreases at 30 and 40°C to approximately 60% of the maximum specific activity observed at 37°C Lactococcus lactis
4.1.2.22 30 40 the activity sharply decreases at 30 and 40°C to approximately 60% of the maximum specific activity observed at 37°C Leuconostoc mesenteroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.9 13.1
-
D-fructose 6-phosphate pH 6.5, 37°C Pseudomonas aeruginosa
4.1.2.9 13.2
-
D-xylulose 5-phosphate pH 6.5, 37°C Pseudomonas aeruginosa
4.1.2.9 44.2
-
D-fructose 6-phosphate pH 6.5, 37°C Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.9 117.8
-
D-fructose 6-phosphate pH 6.5, 37°C Lactococcus lactis subsp. lactis
4.1.2.9 431.8
-
D-xylulose 5-phosphate pH 6.5, 37°C Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.9 587.8
-
D-xylulose 5-phosphate pH 6.5, 37°C Lactococcus lactis subsp. lactis
4.1.2.22 13.05
-
D-fructose 6-phosphate at pH 6.5 and 37°C Pseudomonas aeruginosa
4.1.2.22 13.1
-
D-fructose 6-phosphate pH 6.5, 37°C Pseudomonas aeruginosa
4.1.2.22 13.18
-
D-xylulose 5-phosphate at pH 6.5 and 37°C Pseudomonas aeruginosa
4.1.2.22 13.2
-
D-xylulose 5-phosphate pH 6.5, 37°C Pseudomonas aeruginosa
4.1.2.22 44.2
-
D-fructose 6-phosphate pH 6.5, 37°C Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.22 44.23
-
D-fructose 6-phosphate at pH 6.5 and 37°C Leuconostoc mesenteroides
4.1.2.22 117.76
-
D-fructose 6-phosphate at pH 6.5 and 37°C Lactococcus lactis
4.1.2.22 117.8
-
D-fructose 6-phosphate pH 6.5, 37°C Lactococcus lactis subsp. lactis
4.1.2.22 431.78
-
D-xylulose 5-phosphate at pH 6.5 and 37°C Leuconostoc mesenteroides
4.1.2.22 431.8
-
D-xylulose 5-phosphate pH 6.5, 37°C Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.22 587.76
-
D-xylulose 5-phosphate at pH 6.5 and 37°C Lactococcus lactis
4.1.2.22 587.8
-
D-xylulose 5-phosphate pH 6.5, 37°C Lactococcus lactis subsp. lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.22 6.5
-
-
Lactococcus lactis
4.1.2.22 7
-
-
Pseudomonas aeruginosa
4.1.2.22 7
-
-
Leuconostoc mesenteroides

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.9 thiamine diphosphate
-
Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.9 thiamine diphosphate
-
Pseudomonas aeruginosa
4.1.2.9 thiamine diphosphate
-
Lactococcus lactis subsp. lactis
4.1.2.22 thiamine diphosphate
-
Pseudomonas aeruginosa
4.1.2.22 thiamine diphosphate
-
Lactococcus lactis
4.1.2.22 thiamine diphosphate
-
Leuconostoc mesenteroides
4.1.2.22 thiamine diphosphate
-
Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.22 thiamine diphosphate
-
Lactococcus lactis subsp. lactis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.2.22 Leuconostoc mesenteroides calculated from amino acid sequence
-
5

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.9 0.96
-
D-fructose 6-phosphate pH 6.5, 37°C Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.9 7.43
-
D-fructose 6-phosphate pH 6.5, 37°C Pseudomonas aeruginosa
4.1.2.9 11.9
-
D-fructose 6-phosphate pH 6.5, 37°C Lactococcus lactis subsp. lactis
4.1.2.9 193.15
-
D-xylulose 5-phosphate pH 6.5, 37°C Lactococcus lactis subsp. lactis
4.1.2.9 213.43
-
D-xylulose 5-phosphate pH 6.5, 37°C Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.9 378.62
-
D-xylulose 5-phosphate pH 6.5, 37°C Pseudomonas aeruginosa
4.1.2.22 0.96
-
D-fructose 6-phosphate pH 6.5, 37°C Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.22 0.96
-
D-fructose 6-phosphate at pH 6.5 and 37°C Leuconostoc mesenteroides
4.1.2.22 7.43
-
D-fructose 6-phosphate pH 6.5, 37°C Pseudomonas aeruginosa
4.1.2.22 7.43
-
D-fructose 6-phosphate at pH 6.5 and 37°C Pseudomonas aeruginosa
4.1.2.22 11.9
-
D-fructose 6-phosphate pH 6.5, 37°C Lactococcus lactis subsp. lactis
4.1.2.22 11.9
-
D-fructose 6-phosphate at pH 6.5 and 37°C Lactococcus lactis
4.1.2.22 193.15
-
D-xylulose 5-phosphate pH 6.5, 37°C Lactococcus lactis subsp. lactis
4.1.2.22 193.15
-
D-xylulose 5-phosphate at pH 6.5 and 37°C Lactococcus lactis
4.1.2.22 213.43
-
D-xylulose 5-phosphate pH 6.5, 37°C Leuconostoc mesenteroides subsp. mesenteroides
4.1.2.22 213.43
-
D-xylulose 5-phosphate at pH 6.5 and 37°C Leuconostoc mesenteroides
4.1.2.22 378.62
-
D-xylulose 5-phosphate pH 6.5, 37°C Pseudomonas aeruginosa
4.1.2.22 378.62
-
D-xylulose 5-phosphate at pH 6.5 and 37°C Pseudomonas aeruginosa