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Literature summary extracted from
- Efficient production of thermostable Thermus thermophilus xylose isomerase in Escherichia coli and Bacillus brevis (1992), Appl. Microbiol. Biotechnol., 36, 727-732 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.1.5 | the production of the cloned gene in Escherichia coli and Bacillus brevis are compared expression in Escherichia coli and Bacillus brevis. Bacillus brevis is able to produce the isomerase efficiently (more than 1 g/l) | Thermus thermophilus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 5.3.1.5 | smaller stabilizing effect, while other metals have no effect | Thermus thermophilus |
| 5.3.1.5 | stability is also strongly influenced by the addition of divalent cations. The addition of Mn2+ gives the highest thermostability, Mg2+ has a | Thermus thermophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.1.5 | Thermus thermophilus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.1.5 | - |
Thermus thermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.5 | 95 | - |
- |
Thermus thermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.5 | additional information | - |
stability is also strongly influenced by the addition of divalent cations. The addition of Mn2+ gives the highest thermostability, Mg2+ has a smaller stabilizing effect, while other metals have no effect | Thermus thermophilus |
| 5.3.1.5 | 85 | - |
with Mg2+, half-life: 20 h | Thermus thermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.5 | 7 | - |
- |
Thermus thermophilus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.3.1.5 | 7 | - |
pH stability at 85°C is optimal around pH 7.0 | Thermus thermophilus |
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Release 2023.1 (January 2023)
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