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Literature summary extracted from

  • Cheng, Y.; Wang, D.; Gu, J.; Li, J.; Liu, H.; Li, F.; Han, W.
    Biochemical characteristics and variable alginate-degrading modes of a novel bifunctional endolytic alginate lyase (2017), Appl. Environ. Microbiol., 83, e01608 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.3 T185N NCR-truncated protein, mutant has lost its M-producing capability but retained the ability to yield L-guluronate units by almost completely degrading 2-aminobenzamide-labeled tetra(L-guluronate) chains. Mutant T185N can degrade 2-aminobenzamide-labeled penta(L-guluronate) and 2-aminobenzamide-labeled penta(D-mannuronate) fractions at greater proportions than wild-type Flammeovirga sp. MY04
4.2.2.11 T185N NCR-truncated protein, mutant has lost its M-producing capability but retained the ability to yield G units by almost completely degrading 2-aminobenzamide-labeled G4 chains. Mutant T185N can degrade 2-aminobenzamide-labeled G5 and 2-aminobenzamide-labeled M5 fractions at greater proportions than wild-type Flammeovirga sp. MY04

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.3 Flammeovirga sp. MY04 A0A1B1FUW4 bifunctional alginate lyase, catalyzes the reactions of EC 4.2.2.3 and EC 4.2.2.11
-
4.2.2.11 Flammeovirga sp. MY04 A0A1B1FUW4 bifunctional alginate lyase, catalyzes the reactions of EC 4.2.2.3 and EC 4.2.2.11
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.3 alginate
-
 Flammeovirga sp. MY04 unsaturated algino-oligosaccharides
-
 ?
4.2.2.3 additional information Aly1 is a bifunctional alginate lyase and prefers L-guluronate to D-mannuronate. Tetrasaccharide-size fractions are the smallest substrates, and D-mannuronate, L-guluronate, and UDP2 fractions are the minimal product types. Products are a series of small size-defined saturated oligosaccharide products from the nonreducing ends of single or different saturated sugar chains and yielding unsaturated products in distinct but restricted pattern. No substrates: chondroitin, chondroitin sulfate, dermantan sulfate B, hyaluronan, heparin, or heparin sulfate Flammeovirga sp. MY04 ?
-
 ?
4.2.2.3 poly(alpha-L-(1,4)-guluronate) reaction of EC 4.2.2.11 Flammeovirga sp. MY04 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
4.2.2.3 poly(beta-D-mannuronate)
-
 Flammeovirga sp. MY04 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?
4.2.2.11 alginate
-
 Flammeovirga sp. MY04 unsaturated algino-oligosaccharides
-
 ?
4.2.2.11 additional information Aly1 is a bifunctional alginate lyase and prefers G to M. Tetrasaccharide-size fractions are the smallest substrates, and D-mannuronate, L-guluronate, and UDP2 fractions are the minimal product types. Products are a series of small size-defined saturated oligosaccharide products from the nonreducing ends of single or different saturated sugar chains and yielding unsaturated products in distinct but restricted pattern. No substrates: chondroitin, chondroitin sulfate, dermantan sulfate B, hyaluronan, heparin, or heparin sulfate Flammeovirga sp. MY04 ?
-
 ?
4.2.2.11 poly(alpha-L-1,4-guluronate)
-
 Flammeovirga sp. MY04 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate)
-
 ?
4.2.2.11 poly(beta-D-mannuronate)
-
 Flammeovirga sp. MY04 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate)
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.3 ? x * 50650, calculated from sequence Flammeovirga sp. MY04
4.2.2.11 ? x * 50650, calculated from sequence Flammeovirga sp. MY04

Synonyms

EC Number Synonyms Comment Organism
4.2.2.3 Aly1
-
 Flammeovirga sp. MY04
4.2.2.11 Aly1
-
 Flammeovirga sp. MY04

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.3 50
-
-
 Flammeovirga sp. MY04
4.2.2.11 50
-
-
 Flammeovirga sp. MY04

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.3
-
 40 24 h, at least 60% residual activity Flammeovirga sp. MY04
4.2.2.11
-
 40 24 h, at least 60% residual activity Flammeovirga sp. MY04

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.3 6
-
-
 Flammeovirga sp. MY04
4.2.2.11 6
-
-
 Flammeovirga sp. MY04

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.3 5 8 2 h, at least 80% residual activity Flammeovirga sp. MY04
4.2.2.11 5 8 2 h, at least 80% residual activity Flammeovirga sp. MY04

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.3 Flammeovirga sp. MY04 calculated from sequence
-
 4.2
4.2.2.11 Flammeovirga sp. MY04 calculated from sequence
-
 4.2

General Information

EC Number General Information Comment Organism
4.2.2.3 physiological function deletion of the noncatalytic region of Aly1 causes weak changes of biochemical characteristics but increases the degradation proportions of small size-defined saturated D-mannuronate-enriched oligosaccharide substrates and unsaturated tetrasaccharide fractions without any size changes of degradable oligosaccharides Flammeovirga sp. MY04
4.2.2.11 physiological function deletion of the noncatalytic region of Aly1 causes weak changes of biochemical characteristics but increases the degradation proportions of small size-defined saturated M-enriched oligosaccharide substrates and unsaturated tetrasaccharide fractions without any size changes of degradable oligosaccharides Flammeovirga sp. MY04