EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.99.31 | overexpression in Saccharomyces cerevisiae. Yeast cells expressing CrtI, CrtYB, and CrtS from Xanthophyllomyces dendrorhous accumulate beta-carotene but not astaxanthin | Phaffia rhodozyma |
1.14.99.63 | Saccharomyces cerevisiae cells expressing CrtI, CrtYB, and CrtS from Xanthophyllomyces dendrorhous accumulate beta-carotene but not astaxanthin. Coexpression of CrtR with CrtS results in astaxanthin production in Saccharomyces cerevisiae | Phaffia rhodozyma |
2.5.1.29 | overexpression of geranylgeranyl pyrophosphate synthase from Saccharomyces cerevisiae (the BTS1 gene product) increases the intracellular beta-carotene levels due to the accelerated conversion of farnesyl pyrophosphate to geranylgeranyl diphosphate. Expression of BTS1 in Sacharomyces cerevisiae under the control of a strong and constitutive ADH1 promoter, leads to a 22fold increase in beta-carotene production | Saccharomyces cerevisiae |
2.5.1.32 | overexpression in Saccharomyces cerevisiae. Yeast cells expressing CrtI, CrtYB, and CrtS from Xanthophyllomyces dendrorhous accumulate beta-carotene but not astaxanthin | Phaffia rhodozyma |
5.5.1.19 | overexpression in Saccharomyces cerevisiae. Yeast cells expressing CrtI, CrtYB, and CrtS from Xanthophyllomyces dendrorhous accumulate beta-carotene but not astaxanthin | Phaffia rhodozyma |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.99.63 | cytosol | - |
Phaffia rhodozyma | 5829 | - |
1.14.99.63 | endoplasmic reticulum | - |
Phaffia rhodozyma | 5783 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.99.31 | Phaffia rhodozyma | O13506 | - |
- |
1.3.99.31 | Phaffia rhodozyma ATCC 24202 | O13506 | - |
- |
1.14.99.63 | Phaffia rhodozyma | - |
- |
- |
1.14.99.63 | Phaffia rhodozyma ATCC 24202 | - |
- |
- |
2.5.1.29 | Saccharomyces cerevisiae | - |
- |
- |
2.5.1.29 | Saccharomyces cerevisiae INVSc1 | - |
- |
- |
2.5.1.32 | Phaffia rhodozyma | Q7Z859 | - |
- |
2.5.1.32 | Phaffia rhodozyma ATCC 24202 | Q7Z859 | - |
- |
5.5.1.19 | Phaffia rhodozyma | Q7Z859 | - |
- |
5.5.1.19 | Phaffia rhodozyma ATCC 24202 | Q7Z859 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.99.63 | beta-carotene + 2 reduced acceptor + 2 O2 | the enzyme from the yeast Xanthophyllomyces dendrorhous is bifunctional and also catalyses the activity of beta-carotene 3-hydroxylase | Phaffia rhodozyma | echinenone + 2 acceptor + 3 H2O | - |
? | |
1.14.99.63 | beta-carotene + 2 reduced acceptor + 2 O2 | the enzyme from the yeast Xanthophyllomyces dendrorhous is bifunctional and also catalyses the activity of beta-carotene 3-hydroxylase | Phaffia rhodozyma ATCC 24202 | echinenone + 2 acceptor + 3 H2O | - |
? | |
1.14.99.63 | echinenone + 2 reduced acceptor + 2 O2 | the enzyme from the yeast Xanthophyllomyces dendrorhous is bifunctional and also catalyses the activity of beta-carotene 3-hydroxylase | Phaffia rhodozyma | canthaxanthin + 2 acceptor + 3 H2O | - |
? | |
1.14.99.63 | echinenone + 2 reduced acceptor + 2 O2 | the enzyme from the yeast Xanthophyllomyces dendrorhous is bifunctional and also catalyses the activity of beta-carotene 3-hydroxylase | Phaffia rhodozyma ATCC 24202 | canthaxanthin + 2 acceptor + 3 H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.99.31 | CrtI | - |
Phaffia rhodozyma |
1.3.99.31 | lycopene synthase | - |
Phaffia rhodozyma |
1.3.99.31 | phytoene desaturase | - |
Phaffia rhodozyma |
1.14.99.63 | crtS | - |
Phaffia rhodozyma |
2.5.1.29 | BTS1 | - |
Saccharomyces cerevisiae |
2.5.1.29 | geranylgeranyl pyrophosphate synthase | - |
Saccharomyces cerevisiae |
2.5.1.29 | GGPP synthase | - |
Saccharomyces cerevisiae |
2.5.1.32 | crtYB | - |
Phaffia rhodozyma |
2.5.1.32 | phytoene synthase/lycopene cyclase | - |
Phaffia rhodozyma |
5.5.1.19 | crtYB | - |
Phaffia rhodozyma |
5.5.1.19 | phytoene synthase/lycopene cyclase | - |
Phaffia rhodozyma |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.99.63 | cytochrome P450 | cytochrome P450 protein | Phaffia rhodozyma |