Literature summary extracted from

Hidalgo, A.; Betancor, L.; Moreno, R.; Zafra, O.; Cava, F.; Fernandez-Lafuente, R.; Guisan, J.; Berenguer, J.
Thermus thermophilus as a cell factory for the production of a thermophilic Mn-dependent catalase which fails to be synthesized in an active form in Escherichia coli (2004), Appl. Environ. Microbiol., 70, 3839-3844 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.11.1.6	when the wild-type and mutant (M129V/E293G) genes are overexpressed in Escherichia coli, unmodified or six-His-tagged proteins of the expected size are overproduced as inactive proteins. To overcome this problem, the Mn2+-dependent catalase genes are overexpressed directly in Thermus thermophilus under the control of the Pnar promoter. This promoter belongs to a respiratory nitrate reductase from of Thermus thermophilus HB8, whose transcription is activated by the combined action of nitrate and anoxia. Upon induction in Thermus thermophilus HB8, a 20fold to 30fold increase in catalase specific activity is observed, whereas a 90fold to 110fold increase is detected when the laboratory strain Thermus thermophilus HB27::nar	Thermus thermophilus

Protein Variants

EC Number	Protein Variants	Comment	Organism
1.11.1.6	M129V/E293G	less thermostable mutant enzyme	Thermus thermophilus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.11.1.6	Thermus thermophilus	Q84DB4	-	-

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Release 2023.1 (January 2023)