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Literature summary extracted from

  • Liu, Y.; Song, J.; Tan, T.; Liu, L.
    Production of fumaric acid from L-malic acid by solvent engineering using a recombinant thermostable fumarase from Thermus thermophilus HB8 (2015), Appl. Biochem. Biotechnol., 175, 2823-2831 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.1.2 synthesis synthesis of fumarate in 50% ethylene glycol to shift the reaction equilibrium to fumaric acid. 54.7% conversion is observed using fumarase for transforming 1 mmol L-malic acid. 27% total yield is obtained with 99% purity Thermus thermophilus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.2.1.2 Ethylene glycol in presence of 70 % ethylene glycol, about 47 % L-malic acid is converted to fumaric acid, at 50 % ethylene glycol 38 % conversion occurs and at 30 % ethylene glycol 32 % conversion, respectively. After 2 days, no significant loss of activity in 50 % ethylene glycol is observed, in 70 % ethylene glycol, 80 % activity of the fumarase remain Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Thermus thermophilus Q5SKT5
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.2 385
-
 pH 7.8, 70°C Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 (S)-malate
-
 Thermus thermophilus fumarate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.2 ? x * 50000, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
4.2.1.2 FumC
-
 Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.2 70
-
 30 min, no significant loss of activity Thermus thermophilus