EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.20 | - |
Pyrococcus furiosus |
4.2.1.20 | - |
Escherichia coli |
4.2.1.20 | - |
Thermotoga maritima |
4.2.1.20 | - |
Archaeoglobus fulgidus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.20 | M144T/N166D | 5fold increase in catalytic efficiency | Pyrococcus furiosus |
4.2.1.20 | M145T/N167D | mutant accepts various substrates with substitutions in position 5 of indole | Thermotoga maritima |
4.2.1.20 | M149T/N171D | 2fold increase in catalytic efficiency | Escherichia coli |
4.2.1.20 | M156T/N178D | 5fold increase in catalytic efficiency | Archaeoglobus fulgidus |
4.2.1.20 | P12L/E17G/I68V/F274S/T292S/T321A | 9fold increase in kcat with an equivalent decrease in KM | Pyrococcus furiosus |
4.2.1.20 | P14L/P19G/I69V/L274S/T292S | 2fold decrease in catalytic efficiency | Thermotoga maritima |
4.2.1.20 | P19G/I69V/T292S | 10fold increase in catalytic efficiency | Thermotoga maritima |
4.2.1.20 | P25L/P30G/I80V/L285S/T303S/T321A | 18fold increase in catalytic efficiency | Archaeoglobus fulgidus |
4.2.1.20 | T292S | 7fold increase in catalytic efficiency | Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.20 | 0.009 | - |
indole | mutant P12L/E17G/I68V/F274S/T292S/T321A, pH 8.0, 75°C | Pyrococcus furiosus | |
4.2.1.20 | 0.011 | - |
indole | mutant M156T/N178D, pH 8.0, 60°C | Archaeoglobus fulgidus | |
4.2.1.20 | 0.012 | - |
indole | wild-type, pH 8.0, 60°C | Archaeoglobus fulgidus | |
4.2.1.20 | 0.018 | - |
indole | mutant M149T/N171D, pH 8.0, 37°C | Escherichia coli | |
4.2.1.20 | 0.019 | - |
indole | wild-type, pH 8.0, 37°C | Escherichia coli | |
4.2.1.20 | 0.025 | - |
indole | mutant T292S, pH 8.0, 75°C | Thermotoga maritima | |
4.2.1.20 | 0.026 | - |
indole | mutant P19G/I69V/T292S, pH 8.0, 75°C | Thermotoga maritima | |
4.2.1.20 | 0.032 | - |
indole | mutant M145T/N167D, pH 8.0, 75°C | Thermotoga maritima | |
4.2.1.20 | 0.033 | - |
indole | wild-type, pH 8.0, 75°C | Thermotoga maritima | |
4.2.1.20 | 0.042 | - |
indole | mutant M144T/N166D, pH 8.0, 75°C | Pyrococcus furiosus | |
4.2.1.20 | 0.048 | - |
indole | mutant P25L/P30G/I80V/L285S/T303S/T321A, pH 8.0, 60°C | Archaeoglobus fulgidus | |
4.2.1.20 | 0.072 | - |
indole | mutant P14L/P19G/I69V/L274S/T292S, pH 8.0, 75°C | Thermotoga maritima | |
4.2.1.20 | 0.077 | - |
indole | wild-type, pH 8.0, 75°C | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.20 | Archaeoglobus fulgidus | O28672 | - |
- |
4.2.1.20 | Escherichia coli | P0A879 | - |
- |
4.2.1.20 | Pyrococcus furiosus | Q8U093 | - |
- |
4.2.1.20 | Thermotoga maritima | P50909 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.20 | L-serine + 1H-indole-5-carbaldehyde | substrate of mutant M145T/N167D, 32% yield | Thermotoga maritima | 5-formyl-L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + 1H-indole-5-carbaldehyde | substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A | Pyrococcus furiosus | 5-formyl-L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + 5-bromoindole | - |
Pyrococcus furiosus | 5-bromo-L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + 5-bromoindole | substrate of mutant M145T/N167D, 25% yield | Thermotoga maritima | 5-bromo-L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + 5-bromoindole | substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A | Pyrococcus furiosus | 5-bromo-L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + 5-chloroindole | substrate of mutant M145T/N167D, 93% yield | Thermotoga maritima | 5-chloro-L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + 5-chloroindole | substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A | Pyrococcus furiosus | 5-chloro-L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + 5-cyanoindole | substrate of mutant M145T/N167D, 49% yield | Thermotoga maritima | 5-cyano-L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + 5-hydroxyindole | substrate of mutant M145T/N167D, 93% yield | Thermotoga maritima | 5-hydroxy-L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + 5-hydroxyindole | substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A | Pyrococcus furiosus | 5-hydroxy-L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + 5-methoxyindole | substrate of mutant M145T/N167D, 76% yield | Thermotoga maritima | 5-methoxy-L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + 5-methoxyindole | substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A | Pyrococcus furiosus | 5-methoxy-L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + 5-methylindole | substrate of mutant M145T/N167D, 91% yield | Thermotoga maritima | 5-methyl-L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + 5-methylindole | substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A | Pyrococcus furiosus | 5-methyl-L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + 5-nitroindole | substrate of mutant M145T/N167D, 88% yield | Thermotoga maritima | 5-nitro-L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + 5-nitroindole | substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A | Pyrococcus furiosus | 5-nitro-L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + indole | - |
Pyrococcus furiosus | L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + indole | - |
Escherichia coli | L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + indole | - |
Thermotoga maritima | L-tryptophan + H2O | - |
? | |
4.2.1.20 | L-serine + indole | - |
Archaeoglobus fulgidus | L-tryptophan + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.20 | trpB | - |
Escherichia coli |
4.2.1.20 | trpB | - |
Thermotoga maritima |
4.2.1.20 | trpB | - |
Archaeoglobus fulgidus |
4.2.1.20 | TrpB1 | - |
Pyrococcus furiosus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.20 | 0.074 | - |
indole | wild-type, pH 8.0, 60°C | Archaeoglobus fulgidus | |
4.2.1.20 | 0.11 | - |
indole | mutant P14L/P19G/I69V/L274S/T292S, pH 8.0, 75°C | Thermotoga maritima | |
4.2.1.20 | 0.16 | - |
indole | wild-type, pH 8.0, 37°C | Escherichia coli | |
4.2.1.20 | 0.31 | - |
indole | wild-type, pH 8.0, 75°C | Pyrococcus furiosus | |
4.2.1.20 | 0.34 | - |
indole | mutant M149T/N171D, pH 8.0, 37°C | Escherichia coli | |
4.2.1.20 | 0.34 | - |
indole | mutant M156T/N178D, pH 8.0, 60°C | Archaeoglobus fulgidus | |
4.2.1.20 | 0.51 | - |
indole | mutant P25L/P30G/I80V/L285S/T303S/T321A, pH 8.0, 60°C | Archaeoglobus fulgidus | |
4.2.1.20 | 0.83 | - |
indole | mutant M144T/N166D, pH 8.0, 75°C | Pyrococcus furiosus | |
4.2.1.20 | 1.28 | - |
indole | wild-type, pH 8.0, 75°C | Thermotoga maritima | |
4.2.1.20 | 2.9 | - |
indole | mutant P12L/E17G/I68V/F274S/T292S/T321A, pH 8.0, 75°C | Pyrococcus furiosus | |
4.2.1.20 | 3.3 | - |
indole | mutant M145T/N167D, pH 8.0, 75°C | Thermotoga maritima | |
4.2.1.20 | 5.8 | - |
indole | mutant T292S, pH 8.0, 75°C | Thermotoga maritima | |
4.2.1.20 | 9.8 | - |
indole | mutant P19G/I69V/T292S, pH 8.0, 75°C | Thermotoga maritima |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.20 | 4 | - |
indole | wild-type, pH 8.0, 75°C | Pyrococcus furiosus | |
4.2.1.20 | 6 | - |
indole | wild-type, pH 8.0, 60°C | Archaeoglobus fulgidus | |
4.2.1.20 | 8 | - |
indole | wild-type, pH 8.0, 37°C | Escherichia coli | |
4.2.1.20 | 19 | - |
indole | mutant M149T/N171D, pH 8.0, 37°C | Escherichia coli | |
4.2.1.20 | 20 | - |
indole | mutant M144T/N166D, pH 8.0, 75°C | Pyrococcus furiosus | |
4.2.1.20 | 20 | - |
indole | mutant P14L/P19G/I69V/L274S/T292S, pH 8.0, 75°C | Thermotoga maritima | |
4.2.1.20 | 30 | - |
indole | mutant P12L/E17G/I68V/F274S/T292S/T321A, pH 8.0, 75°C | Pyrococcus furiosus | |
4.2.1.20 | 31 | - |
indole | mutant M156T/N178D, pH 8.0, 60°C | Archaeoglobus fulgidus | |
4.2.1.20 | 39 | - |
indole | wild-type, pH 8.0, 75°C | Thermotoga maritima | |
4.2.1.20 | 100 | - |
indole | mutant M145T/N167D, pH 8.0, 75°C | Thermotoga maritima | |
4.2.1.20 | 110 | - |
indole | mutant P25L/P30G/I80V/L285S/T303S/T321A, pH 8.0, 60°C | Archaeoglobus fulgidus | |
4.2.1.20 | 230 | - |
indole | mutant T292S, pH 8.0, 75°C | Thermotoga maritima | |
4.2.1.20 | 380 | - |
indole | mutant P19G/I69V/T292S, pH 8.0, 75°C | Thermotoga maritima |