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Literature summary extracted from

  • Murciano-Calles, J.; Romney, D.; Brinkmann-Chen, S.; Buller, A.; Arnold, F.
    A Panel of TrpB biocatalysts derived from tryptophan synthase through the transfer of mutations that mimic allosteric activation (2016), Angew. Chem. Int. Ed. Engl., 55, 11577-11581 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.20
-
Pyrococcus furiosus
4.2.1.20
-
Escherichia coli
4.2.1.20
-
Thermotoga maritima
4.2.1.20
-
Archaeoglobus fulgidus

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.20 M144T/N166D 5fold increase in catalytic efficiency Pyrococcus furiosus
4.2.1.20 M145T/N167D mutant accepts various substrates with substitutions in position 5 of indole Thermotoga maritima
4.2.1.20 M149T/N171D 2fold increase in catalytic efficiency Escherichia coli
4.2.1.20 M156T/N178D 5fold increase in catalytic efficiency Archaeoglobus fulgidus
4.2.1.20 P12L/E17G/I68V/F274S/T292S/T321A 9fold increase in kcat with an equivalent decrease in KM Pyrococcus furiosus
4.2.1.20 P14L/P19G/I69V/L274S/T292S 2fold decrease in catalytic efficiency Thermotoga maritima
4.2.1.20 P19G/I69V/T292S 10fold increase in catalytic efficiency Thermotoga maritima
4.2.1.20 P25L/P30G/I80V/L285S/T303S/T321A 18fold increase in catalytic efficiency Archaeoglobus fulgidus
4.2.1.20 T292S 7fold increase in catalytic efficiency Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.20 0.009
-
indole mutant P12L/E17G/I68V/F274S/T292S/T321A, pH 8.0, 75°C Pyrococcus furiosus
4.2.1.20 0.011
-
indole mutant M156T/N178D, pH 8.0, 60°C Archaeoglobus fulgidus
4.2.1.20 0.012
-
indole wild-type, pH 8.0, 60°C Archaeoglobus fulgidus
4.2.1.20 0.018
-
indole mutant M149T/N171D, pH 8.0, 37°C Escherichia coli
4.2.1.20 0.019
-
indole wild-type, pH 8.0, 37°C Escherichia coli
4.2.1.20 0.025
-
indole mutant T292S, pH 8.0, 75°C Thermotoga maritima
4.2.1.20 0.026
-
indole mutant P19G/I69V/T292S, pH 8.0, 75°C Thermotoga maritima
4.2.1.20 0.032
-
indole mutant M145T/N167D, pH 8.0, 75°C Thermotoga maritima
4.2.1.20 0.033
-
indole wild-type, pH 8.0, 75°C Thermotoga maritima
4.2.1.20 0.042
-
indole mutant M144T/N166D, pH 8.0, 75°C Pyrococcus furiosus
4.2.1.20 0.048
-
indole mutant P25L/P30G/I80V/L285S/T303S/T321A, pH 8.0, 60°C Archaeoglobus fulgidus
4.2.1.20 0.072
-
indole mutant P14L/P19G/I69V/L274S/T292S, pH 8.0, 75°C Thermotoga maritima
4.2.1.20 0.077
-
indole wild-type, pH 8.0, 75°C Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.20 Archaeoglobus fulgidus O28672
-
-
4.2.1.20 Escherichia coli P0A879
-
-
4.2.1.20 Pyrococcus furiosus Q8U093
-
-
4.2.1.20 Thermotoga maritima P50909
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.20 L-serine + 1H-indole-5-carbaldehyde substrate of mutant M145T/N167D, 32% yield Thermotoga maritima 5-formyl-L-tryptophan + H2O
-
?
4.2.1.20 L-serine + 1H-indole-5-carbaldehyde substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A Pyrococcus furiosus 5-formyl-L-tryptophan + H2O
-
?
4.2.1.20 L-serine + 5-bromoindole
-
Pyrococcus furiosus 5-bromo-L-tryptophan + H2O
-
?
4.2.1.20 L-serine + 5-bromoindole substrate of mutant M145T/N167D, 25% yield Thermotoga maritima 5-bromo-L-tryptophan + H2O
-
?
4.2.1.20 L-serine + 5-bromoindole substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A Pyrococcus furiosus 5-bromo-L-tryptophan + H2O
-
?
4.2.1.20 L-serine + 5-chloroindole substrate of mutant M145T/N167D, 93% yield Thermotoga maritima 5-chloro-L-tryptophan + H2O
-
?
4.2.1.20 L-serine + 5-chloroindole substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A Pyrococcus furiosus 5-chloro-L-tryptophan + H2O
-
?
4.2.1.20 L-serine + 5-cyanoindole substrate of mutant M145T/N167D, 49% yield Thermotoga maritima 5-cyano-L-tryptophan + H2O
-
?
4.2.1.20 L-serine + 5-hydroxyindole substrate of mutant M145T/N167D, 93% yield Thermotoga maritima 5-hydroxy-L-tryptophan + H2O
-
?
4.2.1.20 L-serine + 5-hydroxyindole substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A Pyrococcus furiosus 5-hydroxy-L-tryptophan + H2O
-
?
4.2.1.20 L-serine + 5-methoxyindole substrate of mutant M145T/N167D, 76% yield Thermotoga maritima 5-methoxy-L-tryptophan + H2O
-
?
4.2.1.20 L-serine + 5-methoxyindole substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A Pyrococcus furiosus 5-methoxy-L-tryptophan + H2O
-
?
4.2.1.20 L-serine + 5-methylindole substrate of mutant M145T/N167D, 91% yield Thermotoga maritima 5-methyl-L-tryptophan + H2O
-
?
4.2.1.20 L-serine + 5-methylindole substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A Pyrococcus furiosus 5-methyl-L-tryptophan + H2O
-
?
4.2.1.20 L-serine + 5-nitroindole substrate of mutant M145T/N167D, 88% yield Thermotoga maritima 5-nitro-L-tryptophan + H2O
-
?
4.2.1.20 L-serine + 5-nitroindole substrate of mutant P12L/E17G/I68V/F274S/T292S/T321A Pyrococcus furiosus 5-nitro-L-tryptophan + H2O
-
?
4.2.1.20 L-serine + indole
-
Pyrococcus furiosus L-tryptophan + H2O
-
?
4.2.1.20 L-serine + indole
-
Escherichia coli L-tryptophan + H2O
-
?
4.2.1.20 L-serine + indole
-
Thermotoga maritima L-tryptophan + H2O
-
?
4.2.1.20 L-serine + indole
-
Archaeoglobus fulgidus L-tryptophan + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.20 trpB
-
Escherichia coli
4.2.1.20 trpB
-
Thermotoga maritima
4.2.1.20 trpB
-
Archaeoglobus fulgidus
4.2.1.20 TrpB1
-
Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.20 0.074
-
indole wild-type, pH 8.0, 60°C Archaeoglobus fulgidus
4.2.1.20 0.11
-
indole mutant P14L/P19G/I69V/L274S/T292S, pH 8.0, 75°C Thermotoga maritima
4.2.1.20 0.16
-
indole wild-type, pH 8.0, 37°C Escherichia coli
4.2.1.20 0.31
-
indole wild-type, pH 8.0, 75°C Pyrococcus furiosus
4.2.1.20 0.34
-
indole mutant M149T/N171D, pH 8.0, 37°C Escherichia coli
4.2.1.20 0.34
-
indole mutant M156T/N178D, pH 8.0, 60°C Archaeoglobus fulgidus
4.2.1.20 0.51
-
indole mutant P25L/P30G/I80V/L285S/T303S/T321A, pH 8.0, 60°C Archaeoglobus fulgidus
4.2.1.20 0.83
-
indole mutant M144T/N166D, pH 8.0, 75°C Pyrococcus furiosus
4.2.1.20 1.28
-
indole wild-type, pH 8.0, 75°C Thermotoga maritima
4.2.1.20 2.9
-
indole mutant P12L/E17G/I68V/F274S/T292S/T321A, pH 8.0, 75°C Pyrococcus furiosus
4.2.1.20 3.3
-
indole mutant M145T/N167D, pH 8.0, 75°C Thermotoga maritima
4.2.1.20 5.8
-
indole mutant T292S, pH 8.0, 75°C Thermotoga maritima
4.2.1.20 9.8
-
indole mutant P19G/I69V/T292S, pH 8.0, 75°C Thermotoga maritima

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.1.20 4
-
indole wild-type, pH 8.0, 75°C Pyrococcus furiosus
4.2.1.20 6
-
indole wild-type, pH 8.0, 60°C Archaeoglobus fulgidus
4.2.1.20 8
-
indole wild-type, pH 8.0, 37°C Escherichia coli
4.2.1.20 19
-
indole mutant M149T/N171D, pH 8.0, 37°C Escherichia coli
4.2.1.20 20
-
indole mutant M144T/N166D, pH 8.0, 75°C Pyrococcus furiosus
4.2.1.20 20
-
indole mutant P14L/P19G/I69V/L274S/T292S, pH 8.0, 75°C Thermotoga maritima
4.2.1.20 30
-
indole mutant P12L/E17G/I68V/F274S/T292S/T321A, pH 8.0, 75°C Pyrococcus furiosus
4.2.1.20 31
-
indole mutant M156T/N178D, pH 8.0, 60°C Archaeoglobus fulgidus
4.2.1.20 39
-
indole wild-type, pH 8.0, 75°C Thermotoga maritima
4.2.1.20 100
-
indole mutant M145T/N167D, pH 8.0, 75°C Thermotoga maritima
4.2.1.20 110
-
indole mutant P25L/P30G/I80V/L285S/T303S/T321A, pH 8.0, 60°C Archaeoglobus fulgidus
4.2.1.20 230
-
indole mutant T292S, pH 8.0, 75°C Thermotoga maritima
4.2.1.20 380
-
indole mutant P19G/I69V/T292S, pH 8.0, 75°C Thermotoga maritima