EC Number | Application | Comment | Organism |
---|---|---|---|
4.2.1.42 | analysis | a continuous assay for L-talarate/galactarate dehydratase is develped using circular dichroism. The advantages that this assay method offers are that initial rate measurements may be obtained much more rapidly than is possible using fixed-time assays (e.g., semicarbazide derivatization or NMR methods) and it is much less complex than a coupled assay, which is often limited by the availability and specific properties of the coupling enzymes | Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.42 | analysis | a convenient, direct continuous circular dichroism-based assay is developed for following the L-talarate/galactarate dehydratase-catalyzed conversion of meso-galactarate to 5-dehydro-4-deoxy-D-glucarate. The advantages that this assay method offers are that initial rate measurements may be obtained much more rapidly than is possible using fixed-time assays (e.g., semicarbazide derivatization or NMR methods) and it is much less complex than a coupled assay, which is often limited by the availability and specific properties of the coupling enzymes | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.42 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.42 | expression in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.156 | gene STM3697, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.42 | Tartronate | competitive inhibitor | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.2.1.156 | additional information | tartronate is a much weaker inhibitor of enzyme StTGD than of mandelate racemase | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.2.1.156 | Tartronate | competitive inhibition | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.42 | 0.38 | - |
meso-galactarate | pH 8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.2.1.42 | 0.38 | - |
galactarate | pH 8.0, 25°C, His6-tagged recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.2.1.156 | additional information | - |
additional information | Michaelis-Menten kinetics | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.156 | L-talarate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
4.2.1.156 | L-talarate | Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | - |
5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
4.2.1.156 | additional information | Salmonella enterica subsp. enterica serovar Typhimurium | the enzyme catalyzes the dehydration of either meso-galactarate or L-talarate via a common intermediate to form 5-keto-4-deoxy-D-glucarate (5-KDG) | ? | - |
? | |
4.2.1.156 | additional information | Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | the enzyme catalyzes the dehydration of either meso-galactarate or L-talarate via a common intermediate to form 5-keto-4-deoxy-D-glucarate (5-KDG) | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.42 | Salmonella enterica subsp. enterica serovar Typhimurium | Q8ZL58 | - |
- |
4.2.1.156 | Salmonella enterica subsp. enterica serovar Typhimurium | Q8ZL58 | - |
- |
4.2.1.156 | Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | Q8ZL58 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.42 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.156 | recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 DE3 by ultracentrifugation, nickel affinity chromatography, and dialysis | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.42 | galactarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | (2R,3S)-2,3-dihydroxy-5-oxohexanedioate + H2O | - |
? | |
4.2.1.42 | galactarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | (2R,3S)-2,3-dihydroxy-5-oxohexanedioate + H2O | - |
? | |
4.2.1.42 | L-talarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
4.2.1.42 | L-talarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
4.2.1.42 | meso-galactarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
4.2.1.42 | meso-galactarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
4.2.1.156 | L-talarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
4.2.1.156 | L-talarate | - |
Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
4.2.1.156 | additional information | the enzyme catalyzes the dehydration of either meso-galactarate or L-talarate via a common intermediate to form 5-keto-4-deoxy-D-glucarate (5-KDG) | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
4.2.1.156 | additional information | the enzyme catalyzes the dehydration of either meso-galactarate or L-talarate via a common intermediate to form 5-keto-4-deoxy-D-glucarate (5-KDG) | Salmonella enterica subsp. enterica serovar Typhimurium LT2 / SGSC1412 / ATCC 700720 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.42 | L-talarate/galactarate dehydratase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.156 | L-talarate/galactarate dehydratase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.156 | More | cf. EC 4.2.1.42 | Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.156 | STM3697 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.156 | StTGD | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.156 | TGD | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.42 | 25 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.156 | 25 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.42 | 4.8 | - |
meso-galactarate | pH 8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.2.1.42 | 4.8 | - |
galactarate | pH 8.0, 25°C, His6-tagged recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.42 | 8 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
4.2.1.156 | 8 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.42 | 10.7 | - |
Tartronate | pH 8.0, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.2.1.42 | 10.7 | - |
Tartronate | pH 8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.1.156 | evolution | L-talarate/galactarate dehydratase (TGD) is a member of the mandelate racemase (MR) subgroup of the enolase superfamily of enzymes. Members of the MR subgroup share with other members of the enolase superfamily a common bidomain structure (i.e., (beta/alpha)-7beta-barrel domain and an alpha+beta capping domain) and a common partial reaction (i.e., the metal-assisted, Broensted base-catalyzed abstraction of the alpha-proton from a carboxylate substrate to form an enol(ate) intermediate) | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.42 | 13 | - |
meso-galactarate | pH 8, 25°C | Salmonella enterica subsp. enterica serovar Typhimurium | |
4.2.1.42 | 13 | - |
galactarate | pH 8.0, 25°C, His6-tagged recombinant enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |