BRENDA - Enzyme Database

Structure of Methylobacterium extorquens malyl-CoA lyase CoA-substrate binding correlates with domain shift

Gonzalez, J.M.; Marti-Arbona, R.; Chen, J.C.; Unkefer, C.J.; Acta Crystallogr. Sect. F 73, 79-85 (2017)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.3.24
expressed in Escherichia coli Arctic Express (DE3) cells
Methylorubrum extorquens
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.1.3.24
sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 30%(w/v) PEG 400, 0.2 M MgCl2
Methylorubrum extorquens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.24
(2R)-4-malyl-CoA
competitive inhibitor
Methylorubrum extorquens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.24
Mg2+
dependent on
Methylorubrum extorquens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.24
(2S)-4-malyl-CoA
Methylorubrum extorquens
-
acetyl-CoA + glyoxylate
-
-
r
4.1.3.24
acetyl-CoA + glyoxylate
Methylorubrum extorquens
-
(2S)-4-malyl-CoA
-
-
r
4.1.3.24
propionyl-CoA + glyoxylate
Methylorubrum extorquens
-
(2S)-4-(beta-methyl)-malyl-CoA
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.24
Methylorubrum extorquens
C5B113
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.24
HisTrap column chromatography and Superdex 200 gel filtration
Methylorubrum extorquens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.24
(2S)-4-malyl-CoA
-
746671
Methylorubrum extorquens
acetyl-CoA + glyoxylate
-
-
-
r
4.1.3.24
acetyl-CoA + glyoxylate
-
746671
Methylorubrum extorquens
(2S)-4-malyl-CoA
-
-
-
r
4.1.3.24
propionyl-CoA + glyoxylate
-
746671
Methylorubrum extorquens
(2S)-4-(beta-methyl)-malyl-CoA
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.24
homohexamer
6 * 34000, calculated from amino acid sequence
Methylorubrum extorquens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.24
expressed in Escherichia coli Arctic Express (DE3) cells
Methylorubrum extorquens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.1.3.24
sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 30%(w/v) PEG 400, 0.2 M MgCl2
Methylorubrum extorquens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.24
(2R)-4-malyl-CoA
competitive inhibitor
Methylorubrum extorquens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.24
Mg2+
dependent on
Methylorubrum extorquens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.24
(2S)-4-malyl-CoA
Methylorubrum extorquens
-
acetyl-CoA + glyoxylate
-
-
r
4.1.3.24
acetyl-CoA + glyoxylate
Methylorubrum extorquens
-
(2S)-4-malyl-CoA
-
-
r
4.1.3.24
propionyl-CoA + glyoxylate
Methylorubrum extorquens
-
(2S)-4-(beta-methyl)-malyl-CoA
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.24
HisTrap column chromatography and Superdex 200 gel filtration
Methylorubrum extorquens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.24
(2S)-4-malyl-CoA
-
746671
Methylorubrum extorquens
acetyl-CoA + glyoxylate
-
-
-
r
4.1.3.24
acetyl-CoA + glyoxylate
-
746671
Methylorubrum extorquens
(2S)-4-malyl-CoA
-
-
-
r
4.1.3.24
propionyl-CoA + glyoxylate
-
746671
Methylorubrum extorquens
(2S)-4-(beta-methyl)-malyl-CoA
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.24
homohexamer
6 * 34000, calculated from amino acid sequence
Methylorubrum extorquens