BRENDA - Enzyme Database show

Cloning, expression, purification, crystallization and X-ray diffraction analysis of dihydrodipicolinate synthase from the human pathogenic bacterium Bartonella henselae strain Houston-1 at 2.1 A resolution

Naqvi, K.F.; Staker, B.L.; Dobson, R.C.; Serbzhinskiy, D.; Sankaran, B.; Myler, P.J.; Hudson, A.O.; Acta Crystallogr. Sect. F 72, 2-9 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.3.3.7
-
Bartonella henselae
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.3.3.7
sitting-drop vapor-diffusion; sitting-drop vapor-diffusion, protein crystals are grown in conditions consisting of 20%(w/v) PEG 4000, 100 mM sodium citrate tribasic pH 5.5 and diffract to 2.10 A resolution. They belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 79.96, b = 106.33, c = 136.25 A
Bartonella henselae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
Bartonella henselae
the enzyme catalyzes the committed step in the synthesis of diaminopimelate and lysine to facilitate peptidoglycan and protein synthesis
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
Bartonella henselae ATCC 49882
the enzyme catalyzes the committed step in the synthesis of diaminopimelate and lysine to facilitate peptidoglycan and protein synthesis
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
Bartonella henselae Houston 1
the enzyme catalyzes the committed step in the synthesis of diaminopimelate and lysine to facilitate peptidoglycan and protein synthesis
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.3.3.7
Bartonella henselae
Q6G468
-
-
4.3.3.7
Bartonella henselae ATCC 49882
Q6G468
-
-
4.3.3.7
Bartonella henselae Houston 1
Q6G468
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.3.3.7
-
Bartonella henselae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
-
746659
Bartonella henselae
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
the enzyme catalyzes the committed step in the synthesis of diaminopimelate and lysine to facilitate peptidoglycan and protein synthesis
746659
Bartonella henselae
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
-
746659
Bartonella henselae ATCC 49882
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
the enzyme catalyzes the committed step in the synthesis of diaminopimelate and lysine to facilitate peptidoglycan and protein synthesis
746659
Bartonella henselae ATCC 49882
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
-
746659
Bartonella henselae Houston 1
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
the enzyme catalyzes the committed step in the synthesis of diaminopimelate and lysine to facilitate peptidoglycan and protein synthesis
746659
Bartonella henselae Houston 1
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.3.3.7
tetramer
-
Bartonella henselae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
-
Bartonella henselae
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.3.3.7
sitting-drop vapor-diffusion; sitting-drop vapor-diffusion, protein crystals are grown in conditions consisting of 20%(w/v) PEG 4000, 100 mM sodium citrate tribasic pH 5.5 and diffract to 2.10 A resolution. They belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 79.96, b = 106.33, c = 136.25 A
Bartonella henselae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
Bartonella henselae
the enzyme catalyzes the committed step in the synthesis of diaminopimelate and lysine to facilitate peptidoglycan and protein synthesis
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
Bartonella henselae ATCC 49882
the enzyme catalyzes the committed step in the synthesis of diaminopimelate and lysine to facilitate peptidoglycan and protein synthesis
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
Bartonella henselae Houston 1
the enzyme catalyzes the committed step in the synthesis of diaminopimelate and lysine to facilitate peptidoglycan and protein synthesis
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.3.3.7
-
Bartonella henselae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
-
746659
Bartonella henselae
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
the enzyme catalyzes the committed step in the synthesis of diaminopimelate and lysine to facilitate peptidoglycan and protein synthesis
746659
Bartonella henselae
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
-
746659
Bartonella henselae ATCC 49882
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
the enzyme catalyzes the committed step in the synthesis of diaminopimelate and lysine to facilitate peptidoglycan and protein synthesis
746659
Bartonella henselae ATCC 49882
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
-
746659
Bartonella henselae Houston 1
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
4.3.3.7
pyruvate + L-aspartate-4-semialdehyde
the enzyme catalyzes the committed step in the synthesis of diaminopimelate and lysine to facilitate peptidoglycan and protein synthesis
746659
Bartonella henselae Houston 1
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.3.3.7
tetramer
-
Bartonella henselae
General Information
EC Number
General Information
Commentary
Organism
4.3.3.7
metabolism
the enzyme catalyzes the committed step in the synthesis of diaminopimelate and lysine to facilitate peptidoglycan and protein synthesis
Bartonella henselae
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.3.3.7
metabolism
the enzyme catalyzes the committed step in the synthesis of diaminopimelate and lysine to facilitate peptidoglycan and protein synthesis
Bartonella henselae